PMID- 29737563
OWN - NLM
STAT- MEDLINE
DCOM- 20190515
LR  - 20220408
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 119
IP  - 8
DP  - 2018 Aug
TI  - Long non-coding RNA LOC554202 promotes laryngeal squamous cell carcinoma 
      progression through regulating miR-31.
PG  - 6953-6960
LID - 10.1002/jcb.26902 [doi]
AB  - Laryngeal squamous cell carcinoma (LSCC) is one aggressive malignancy and 
      accounts for 20% of all head and neck cancer. However, the role of LOC554202 in 
      human LSCC remains unknown. The expression level of LOC554202 and miR-31 was 
      detected in the LSCC tiussues by using qRT-PCR. Cell growth was measured by CCK-8 
      assay. Flow cytometry and matrigel-coated membrane was used to detect for cell 
      cycle and invasion respectively. We indicated that lncRNA LOC554202 expression 
      was overexpressed in LSCC tissues compared with the paired adjacent samples and 
      higher LOC554202 expression was associated with the advanced stage. In addition, 
      we demonstrated that the expression level of miR-31 was downregulated in LSCC 
      tissues compared to the paired adjacent samples and lower miR-31 expression was 
      correlated with the advanced stage. Moreover, the expression of miR-31 was 
      negatively correlated with the expression of LOC554202 in LSCC tissues. Ectopic 
      expression of LOC554202 promoted LSCC cell growth, cell cyle and cell invasion 
      and overexpression of miR-31 inhibited LSCC cell growth, cell cyle and cell 
      invasion. Elevated expression of LOC554202 suppressed miR-31 expression and 
      promoted RhoA expression in LSCC cell, which was a direct target gene of miR-31. 
      Furthermore, LOC554202 increased LSCC cell growth, cell cyle and cell invasion 
      through suppressing miR-31 expression. These results suggested that LOC554202 
      acted as an oncogene in the development of LSCC.
CI  - (c) 2018 Wiley Periodicals, Inc.
FAU - Yang, Shujuan
AU  - Yang S
AD  - Department of Otolaryngology, Liaocheng People's Hospital, Liaocheng, Shandong, 
      China.
FAU - Wang, Jing
AU  - Wang J
AUID- ORCID: 0000-0001-8348-2137
AD  - Department of Otolaryngology, Liaocheng People's Hospital, Liaocheng, Shandong, 
      China.
FAU - Ge, Wensheng
AU  - Ge W
AD  - Department of Otolaryngology, Liaocheng People's Hospital, Liaocheng, Shandong, 
      China.
FAU - Jiang, Yanfang
AU  - Jiang Y
AD  - Genetic Diagnosis Center, The First Hospital of Jilin University, Changchun, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180508
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (MIRN31 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Neoplasm)
RN  - 124671-05-2 (RHOA protein, human)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
SB  - IM
MH  - Carcinoma, Squamous Cell/genetics/*metabolism/pathology
MH  - *Cell Cycle
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/*metabolism/pathology
MH  - MicroRNAs/*biosynthesis/genetics
MH  - Neoplasm Invasiveness
MH  - Neoplasm Proteins/biosynthesis/genetics
MH  - RNA, Long Noncoding/*biosynthesis/genetics
MH  - RNA, Neoplasm/*biosynthesis/genetics
MH  - rhoA GTP-Binding Protein/biosynthesis/genetics
OTO - NOTNLM
OT  - LOC554202
OT  - RhoA
OT  - laryngeal squamous cell carcinoma
OT  - miR-31
EDAT- 2018/05/09 06:00
MHDA- 2019/05/16 06:00
CRDT- 2018/05/09 06:00
PHST- 2018/02/05 00:00 [received]
PHST- 2018/03/28 00:00 [accepted]
PHST- 2018/05/09 06:00 [pubmed]
PHST- 2019/05/16 06:00 [medline]
PHST- 2018/05/09 06:00 [entrez]
AID - 10.1002/jcb.26902 [doi]
PST - ppublish
SO  - J Cell Biochem. 2018 Aug;119(8):6953-6960. doi: 10.1002/jcb.26902. Epub 2018 May 
      8.