PMID- 29738111
OWN - NLM
STAT- MEDLINE
DCOM- 20190408
LR  - 20190408
IS  - 1755-148X (Electronic)
IS  - 1755-1471 (Linking)
VI  - 31
IP  - 6
DP  - 2018 Nov
TI  - Raman spectroscopy quantification of eumelanin subunits in natural unaltered 
      pigments.
PG  - 673-682
LID - 10.1111/pcmr.12707 [doi]
AB  - Eumelanins are large polymers composed of 5,6-dihydroxyindole (DHI) and 
      5,6-dihydroxyindole-2-carboxylic acid (DHICA). Reactive oxygen species generation 
      is higher during the synthesis of DHI than during the synthesis of DHICA; thus, 
      the production of eumelanins with high relative DHI contents may compromise the 
      viability of cells and organisms. Here, we show that dispersive Raman 
      spectroscopy allows a noninvasive direct detection and quantification of DHI and 
      DHICA in unaltered eumelanins that can be applied to the analysis of natural 
      pigments in biological tissues. Using synthetic eumelanins purely composed of DHI 
      or DHICA, we develop a model to determine the differential contribution of these 
      subunits to the Raman spectra of mixed eumelanins, and exemplify the predictive 
      capacity of the model with natural eumelanins deposited in black and gray 
      feathers of fowl. We found that the wavenumber ranges of 550-1,200 and 
      1,650-2,300 cm(-1) are significant to predict the DHI:DHICA ratio in unknown 
      samples.
CI  - (c) 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Galvan, Ismael
AU  - Galvan I
AUID- ORCID: 0000-0002-6523-8592
AD  - Departamento de Ecologia Evolutiva, Estacion Biologica de Donana, CSIC, Sevilla, 
      Spain.
FAU - Araujo-Andrade, Cuauhtemoc
AU  - Araujo-Andrade C
AD  - ICFO - Institut de Ciencies Fotoniques, The Barcelona Institute of Science and 
      Technology, Castelldefels, Spain.
AD  - Unidad Academica de Fisica, Universidad Autonoma de Zacatecas, Zacatecas, Mexico.
FAU - Marro, Monica
AU  - Marro M
AD  - ICFO - Institut de Ciencies Fotoniques, The Barcelona Institute of Science and 
      Technology, Castelldefels, Spain.
FAU - Loza-Alvarez, Pablo
AU  - Loza-Alvarez P
AD  - ICFO - Institut de Ciencies Fotoniques, The Barcelona Institute of Science and 
      Technology, Castelldefels, Spain.
FAU - Wakamatsu, Kazumasa
AU  - Wakamatsu K
AUID- ORCID: 0000-0003-1748-9001
AD  - Department of Chemistry, Fujita Health University School of Health Sciences, 
      Toyoake, Japan.
LA  - eng
GR  - RYC-2012-10237/Spanish Ministry of Economy, Industry and Competitiveness 
      (MINECO)/International
GR  - SEV-2015-0522/Spanish Ministry of Economy, Industry and Competitiveness 
      (MINECO)/International
GR  - FIS2016-80455-R/Spanish Ministry of Economy, Industry and Competitiveness 
      (MINECO)/International
GR  - EU-H2020 654148/H2020 Framework/International
GR  - 424/C/2014/Fundacio la Marato de TV3/International
GR  - Fundacio Cellex Barcelona/International
GR  - Generalitat de Catalunya/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180524
PL  - England
TA  - Pigment Cell Melanoma Res
JT  - Pigment cell & melanoma research
JID - 101318927
RN  - 0 (Indoles)
RN  - 0 (Melanins)
RN  - 12627-86-0 (eumelanin)
RN  - 4790-08-3 (5,6-dihydroxy-2-indolylcarboxylic acid)
RN  - Z3OC8499KG (5,6-dihydroxyindole)
SB  - IM
MH  - Animals
MH  - Birds
MH  - Calibration
MH  - Feathers/chemistry
MH  - Indoles/analysis
MH  - Melanins/chemistry/*metabolism
MH  - Pigmentation
MH  - Reproducibility of Results
MH  - Spectrum Analysis, Raman/*methods
OTO - NOTNLM
OT  - DHI
OT  - DHICA
OT  - ROS
OT  - Raman spectroscopy
OT  - eumelanins
OT  - melanogenesis
EDAT- 2018/05/09 06:00
MHDA- 2019/04/09 06:00
CRDT- 2018/05/09 06:00
PHST- 2018/03/02 00:00 [received]
PHST- 2018/04/19 00:00 [revised]
PHST- 2018/04/20 00:00 [accepted]
PHST- 2018/05/09 06:00 [pubmed]
PHST- 2019/04/09 06:00 [medline]
PHST- 2018/05/09 06:00 [entrez]
AID - 10.1111/pcmr.12707 [doi]
PST - ppublish
SO  - Pigment Cell Melanoma Res. 2018 Nov;31(6):673-682. doi: 10.1111/pcmr.12707. Epub 
      2018 May 24.