PMID- 29738283
OWN - NLM
STAT- MEDLINE
DCOM- 20181022
LR  - 20181022
IS  - 1480-3321 (Electronic)
IS  - 0831-2796 (Linking)
VI  - 61
IP  - 7
DP  - 2018 Jul
TI  - Identification of chromosomes in Thinopyrum intermedium and wheat Th. intermedium 
      amphiploids based on multiplex oligonucleotide probes.
PG  - 515-521
LID - 10.1139/gen-2018-0019 [doi]
AB  - Synthesized oligonucleotides (oligos) can be used as effective probes similar to 
      plasmid clones for chromosome identification in fluorescence in situ 
      hybridization (FISH) analysis, making oligo FISH a simpler and more efficient 
      molecular cytogenetic technique for studying plants. In this study, multiplex 
      oligonucleotide probes, including pSc119.2-1, pAs1-4, (GAA)(10), (AAC)(6), and 
      pTa71, were combined and used in FISH to identify chromosomes in common wheat, 
      Thinopyrum intermedium, and a wheat - Th. intermedium amphiploid TE256-1. In 
      comparison with general FISH probes, signals generated by the multiplex probes 
      were more abundant, colorful, and characteristic. Combining the results of 
      genomic in situ hybridization (GISH) with FISH, Th. intermedium chromosomes and 
      alien chromosomes in TE256-1 could be classified and identified more precisely, 
      especially the J- and J(s)-genome chromosomes. Moreover, based on the FISH 
      results using multiplex probes, more structural variations in wheat chromosomes 
      of TE256-1 were detected. The results indicated that multiplex oligo probes would 
      have a wide range of application prospects in the creation and identification of 
      wheat - Th. intermedium germplasms.
FAU - Cui, Yu
AU  - Cui Y
AD  - a State Key Laboratory of Crop Biology, Shandong Agriculture University, Tai'an, 
      Shandong 271018, China.
FAU - Zhang, Yanping
AU  - Zhang Y
AD  - b College of Agronomy, Shandong Agriculture University, Tai'an, Shandong 271018, 
      China.
FAU - Qi, Juan
AU  - Qi J
AD  - a State Key Laboratory of Crop Biology, Shandong Agriculture University, Tai'an, 
      Shandong 271018, China.
FAU - Wang, Honggang
AU  - Wang H
AD  - a State Key Laboratory of Crop Biology, Shandong Agriculture University, Tai'an, 
      Shandong 271018, China.
AD  - b College of Agronomy, Shandong Agriculture University, Tai'an, Shandong 271018, 
      China.
FAU - Wang, Richard R-C
AU  - Wang RR
AD  - c United States Department of Agriculture - Agricultural Research Services, 
      Forage and Range Research Laboratory, Logan, UT 84322-6300, USA.
FAU - Bao, Yinguang
AU  - Bao Y
AD  - b College of Agronomy, Shandong Agriculture University, Tai'an, Shandong 271018, 
      China.
FAU - Li, Xingfeng
AU  - Li X
AD  - a State Key Laboratory of Crop Biology, Shandong Agriculture University, Tai'an, 
      Shandong 271018, China.
AD  - b College of Agronomy, Shandong Agriculture University, Tai'an, Shandong 271018, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20180508
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Chromosomes, Plant/*genetics
MH  - Cytogenetic Analysis/methods
MH  - Genome, Plant/genetics
MH  - Hybridization, Genetic
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Oligonucleotide Probes/*genetics
MH  - *Ploidies
MH  - Poaceae/*genetics
MH  - Reproducibility of Results
MH  - Triticum/*genetics
OTO - NOTNLM
OT  - Thinopyrum intermedium
OT  - chromosome variation
OT  - hybridation in situ
OT  - in situ hybridization
OT  - multiplex probes
OT  - sondes en multiplexe
OT  - variation chromosomique
EDAT- 2018/05/09 06:00
MHDA- 2018/10/23 06:00
CRDT- 2018/05/09 06:00
PHST- 2018/05/09 06:00 [pubmed]
PHST- 2018/10/23 06:00 [medline]
PHST- 2018/05/09 06:00 [entrez]
AID - 10.1139/gen-2018-0019 [doi]
PST - ppublish
SO  - Genome. 2018 Jul;61(7):515-521. doi: 10.1139/gen-2018-0019. Epub 2018 May 8.