PMID- 29743604
OWN - NLM
STAT- MEDLINE
DCOM- 20181217
LR  - 20220129
IS  - 2041-1723 (Electronic)
IS  - 2041-1723 (Linking)
VI  - 9
IP  - 1
DP  - 2018 May 9
TI  - Small GTPases and BAR domain proteins regulate branched actin polymerisation for 
      clathrin and dynamin-independent endocytosis.
PG  - 1835
LID - 10.1038/s41467-018-03955-w [doi]
LID - 1835
AB  - Using real-time TIRF microscopy imaging, we identify sites of clathrin and 
      dynamin-independent CLIC/GEEC (CG) endocytic vesicle formation. This allows 
      spatio-temporal localisation of known molecules affecting CG endocytosis; GBF1 (a 
      GEF for ARF1), ARF1 and CDC42 which appear sequentially over 60 s, preceding 
      scission. In an RNAi screen for BAR domain proteins affecting CG endocytosis, 
      IRSp53 and PICK1, known interactors of CDC42 and ARF1, respectively, were 
      selected. Removal of IRSp53, a negative curvature sensing protein, abolishes CG 
      endocytosis. Furthermore, the identification of ARP2/3 complex at CG endocytic 
      sites, maintained in an inactive state reveals a function for PICK1, an ARP2/3 
      inhibitor. The spatio-temporal sequence of the arrival and disappearance of the 
      molecules suggest a mechanism for a clathrin and dynamin-independent endocytic 
      process. Coincident with the loss of PICK1 by GBF1-activated ARF1, CDC42 
      recruitment leads to the activation of IRSp53 and the ARP2/3 complex, resulting 
      in a burst of F-actin polymerisation potentially powering scission.
FAU - Sathe, Mugdha
AU  - Sathe M
AD  - National Centre for Biological Science (TIFR), Bellary Road, Bangalore, 560065, 
      India.
FAU - Muthukrishnan, Gayatri
AU  - Muthukrishnan G
AD  - National Centre for Biological Science (TIFR), Bellary Road, Bangalore, 560065, 
      India.
FAU - Rae, James
AU  - Rae J
AD  - Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, 
      4072, Australia.
AD  - Centre for Microscopy and Microanalysis, University of Queensland, Brisbane, QLD, 
      4072, Australia.
FAU - Disanza, Andrea
AU  - Disanza A
AD  - IFOM, Fondazione Istituto FIRC di Oncologia Molecolare, Milan, 20139, Italy.
AD  - Department of Oncology and Hemato-Oncology, University of Milan, Milan, 20122, 
      Italy.
FAU - Thattai, Mukund
AU  - Thattai M
AUID- ORCID: 0000-0002-2558-6517
AD  - National Centre for Biological Science (TIFR), Bellary Road, Bangalore, 560065, 
      India.
AD  - Simons Centre for the Study of Living Machines, National Centre for Biological 
      Sciences (TIFR), Bellary Road, Bangalore, 560065, India.
FAU - Scita, Giorgio
AU  - Scita G
AUID- ORCID: 0000-0001-7984-1889
AD  - IFOM, Fondazione Istituto FIRC di Oncologia Molecolare, Milan, 20139, Italy.
AD  - Department of Oncology and Hemato-Oncology, University of Milan, Milan, 20122, 
      Italy.
FAU - Parton, Robert G
AU  - Parton RG
AUID- ORCID: 0000-0002-7494-5248
AD  - Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, 
      4072, Australia.
AD  - Centre for Microscopy and Microanalysis, University of Queensland, Brisbane, QLD, 
      4072, Australia.
FAU - Mayor, Satyajit
AU  - Mayor S
AD  - National Centre for Biological Science (TIFR), Bellary Road, Bangalore, 560065, 
      India. mayor@ncbs.res.in.
AD  - Institute for Stem Cell Biology and Regenerative Medicine, Bellary Road, 
      Bangalore, 560065, India. mayor@ncbs.res.in.
LA  - eng
GR  - Wellcome Trust/United Kingdom
GR  - 268836/ERC_/European Research Council/International
GR  - 500056-Z-09-Z/WTDBT_/DBT-Wellcome Trust India Alliance/India
GR  - IA/M/15/1/502018/WTDBT_/DBT-Wellcome Trust India Alliance/India
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20180509
PL  - England
TA  - Nat Commun
JT  - Nature communications
JID - 101528555
RN  - 0 (Actins)
RN  - 0 (BAIAP2 protein, human)
RN  - 0 (Carrier Proteins)
RN  - 0 (Clathrin)
RN  - 0 (GBF1 protein, human)
RN  - 0 (Guanine Nucleotide Exchange Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (PICk1 protein, human)
RN  - EC 3.6.5.2 (ADP-Ribosylation Factor 1)
RN  - EC 3.6.5.2 (cdc42 GTP-Binding Protein)
RN  - EC 3.6.5.5 (Dynamins)
SB  - IM
EIN - Nat Commun. 2021 Aug 23;12(1):5158. PMID: 34426583
MH  - ADP-Ribosylation Factor 1/genetics/*metabolism
MH  - Actins/*chemistry/metabolism
MH  - Animals
MH  - Carrier Proteins/genetics/*metabolism
MH  - Clathrin/chemistry/*metabolism
MH  - Dynamins/chemistry/*metabolism
MH  - *Endocytosis
MH  - Fibroblasts/enzymology/metabolism
MH  - Guanine Nucleotide Exchange Factors/genetics/metabolism
MH  - Humans
MH  - Mice
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Polymerization
MH  - Protein Binding
MH  - Protein Domains
MH  - cdc42 GTP-Binding Protein/genetics/*metabolism
PMC - PMC5943408
COIS- The authors declare no competing interests.
EDAT- 2018/05/11 06:00
MHDA- 2018/12/18 06:00
PMCR- 2018/05/09
CRDT- 2018/05/11 06:00
PHST- 2017/08/23 00:00 [received]
PHST- 2018/03/22 00:00 [accepted]
PHST- 2018/05/11 06:00 [entrez]
PHST- 2018/05/11 06:00 [pubmed]
PHST- 2018/12/18 06:00 [medline]
PHST- 2018/05/09 00:00 [pmc-release]
AID - 10.1038/s41467-018-03955-w [pii]
AID - 3955 [pii]
AID - 10.1038/s41467-018-03955-w [doi]
PST - epublish
SO  - Nat Commun. 2018 May 9;9(1):1835. doi: 10.1038/s41467-018-03955-w.