PMID- 29746864
OWN - NLM
STAT- MEDLINE
DCOM- 20190513
LR  - 20190513
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 501
IP  - 3
DP  - 2018 Jun 27
TI  - Drug delivery using polyhistidine peptide-modified liposomes that target 
      endogenous lysosome.
PG  - 648-653
LID - S0006-291X(18)31086-6 [pii]
LID - 10.1016/j.bbrc.2018.05.037 [doi]
AB  - Cell-penetrating peptides (CPPs) can deliver payloads into cells by forming 
      complexes with bioactive molecules via covalent or non-covalent bonds. Various 
      CPPs have been applied in CPP-modified liposomes, and their effectiveness is 
      highly regarded in liposomal drug delivery systems (DDSs). Previously, we have 
      reported on the polyhistidine peptide (H16 peptide: HHHHHHHHHHHHHHHH-NH(2)) as a 
      new CPP. The H16 peptide has a higher cell-penetrating capacity than well-known 
      CPPs and delivers small molecules such as fluorescent dyes, bioactive peptides, 
      and proteins into mammalian cells. However, it is not known whether the H16 
      peptide can deliver large cargos such as liposomes into cells. To assess the 
      potential of the H16 peptide, in this study, we developed H16 peptide-modified 
      liposomes (H16-Lipo) and evaluated their effectiveness in a liposomal DDS. The 
      H16-Lipo was prepared by inserting a stearyl-H16 peptide into the hydrophobic 
      region of a liposome. The H16-Lipo was internalized into human fibrosarcoma cells 
      via multiple endocytosis pathways and localized to intracellular lysosomes. Based 
      on this result, we used the H16-Lipo as a lysosome-targeting DDS. The H16-Lipo 
      delivered alpha-galactosidase A (GLA), one of the lysosomal enzymes, to 
      intracellular lysosomes and improved the proliferation of GLA-knockdown cells. 
      These results suggest that the H16-Lipo is an effective drug carrier for 
      lysosomal enzymes in a lysosome-targeting DDS. The loss of lysosomal enzymes has 
      been known to induce metabolic disorders, called lysosomal storage diseases 
      (LSDs). Our findings indicate that this combination of the H16 peptide and a 
      liposome is a promising candidate as a DDS for the treatment of LSDs.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Hayashi, Taiki
AU  - Hayashi T
AD  - Department of Agriculture, Graduate School of Sustainability Science, Tottori 
      University, Tottori 680-8553, Japan.
FAU - Shinagawa, Matsumi
AU  - Shinagawa M
AD  - Department of Bioresource Sciences, Faculty of Agriculture, Tottori University, 
      Tottori 680-8553, Japan.
FAU - Kawano, Tsuyoshi
AU  - Kawano T
AD  - Department of Agriculture, Graduate School of Sustainability Science, Tottori 
      University, Tottori 680-8553, Japan; Department of Bioresource Sciences, Faculty 
      of Agriculture, Tottori University, Tottori 680-8553, Japan.
FAU - Iwasaki, Takashi
AU  - Iwasaki T
AD  - Department of Agriculture, Graduate School of Sustainability Science, Tottori 
      University, Tottori 680-8553, Japan; Department of Bioresource Sciences, Faculty 
      of Agriculture, Tottori University, Tottori 680-8553, Japan. Electronic address: 
      itaka@tottori-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Liposomes)
RN  - 26062-48-6 (polyhistidine)
RN  - 4QD397987E (Histidine)
RN  - EC 3.2.1.22 (alpha-Galactosidase)
SB  - IM
MH  - Cell Line, Tumor
MH  - Drug Delivery Systems
MH  - Histidine/chemistry/*metabolism
MH  - Humans
MH  - Liposomes/chemistry/*metabolism
MH  - Lysosomes/*metabolism
MH  - alpha-Galactosidase/*administration & dosage/pharmacokinetics
OTO - NOTNLM
OT  - Cell-penetrating peptide
OT  - Drug delivery system
OT  - Histidine
OT  - Liposome
OT  - Lysosome
EDAT- 2018/05/11 06:00
MHDA- 2019/05/14 06:00
CRDT- 2018/05/11 06:00
PHST- 2018/04/26 00:00 [received]
PHST- 2018/05/06 00:00 [accepted]
PHST- 2018/05/11 06:00 [pubmed]
PHST- 2019/05/14 06:00 [medline]
PHST- 2018/05/11 06:00 [entrez]
AID - S0006-291X(18)31086-6 [pii]
AID - 10.1016/j.bbrc.2018.05.037 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2018 Jun 27;501(3):648-653. doi: 
      10.1016/j.bbrc.2018.05.037.