PMID- 29748648
OWN - NLM
STAT- MEDLINE
DCOM- 20190415
LR  - 20190415
IS  - 1750-2799 (Electronic)
IS  - 1750-2799 (Linking)
VI  - 13
IP  - 6
DP  - 2018 Jun
TI  - High-yield in vitro recordings from neurons functionally characterized in vivo.
PG  - 1275-1293
LID - 10.1038/nprot.2018.026 [doi]
AB  - In vivo two-photon calcium imaging provides detailed information about the 
      activity and response properties of individual neurons. However, in vitro methods 
      are often required to study the underlying neuronal connectivity and physiology 
      at the cellular and synaptic levels at high resolution. This protocol provides a 
      fast and reliable workflow for combining the two approaches by characterizing the 
      response properties of individual neurons in mice in vivo using genetically 
      encoded calcium indicators (GECIs), followed by retrieval of the same neurons in 
      brain slices for further analysis in vitro (e.g., circuit mapping). In this 
      approach, a reference frame is provided by fluorescent-bead tracks and sparsely 
      transduced neurons expressing a structural marker in order to re-identify the 
      same neurons. The use of GECIs provides a substantial advancement over previous 
      approaches by allowing for repeated in vivo imaging. This opens the possibility 
      of directly correlating experience-dependent changes in neuronal activity and 
      feature selectivity with changes in neuronal connectivity and physiology. This 
      protocol requires expertise both in in vivo two-photon calcium imaging and in 
      vitro electrophysiology. It takes 3 weeks or more to complete, depending on the 
      time allotted for repeated in vivo imaging of neuronal activity.
FAU - Weiler, Simon
AU  - Weiler S
AD  - Max Planck Institute of Neurobiology, Munchen-Martinsried, Germany.
FAU - Bauer, Joel
AU  - Bauer J
AD  - Max Planck Institute of Neurobiology, Munchen-Martinsried, Germany.
FAU - Hubener, Mark
AU  - Hubener M
AUID- ORCID: 0000-0001-8367-9132
AD  - Max Planck Institute of Neurobiology, Munchen-Martinsried, Germany.
FAU - Bonhoeffer, Tobias
AU  - Bonhoeffer T
AUID- ORCID: 0000-0001-7897-6634
AD  - Max Planck Institute of Neurobiology, Munchen-Martinsried, Germany.
FAU - Rose, Tobias
AU  - Rose T
AUID- ORCID: 0000-0002-7156-4714
AD  - Max Planck Institute of Neurobiology, Munchen-Martinsried, Germany.
FAU - Scheuss, Volker
AU  - Scheuss V
AUID- ORCID: 0000-0002-0659-3228
AD  - Max Planck Institute of Neurobiology, Munchen-Martinsried, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180510
PL  - England
TA  - Nat Protoc
JT  - Nature protocols
JID - 101284307
SB  - IM
MH  - Animals
MH  - *Calcium Signaling
MH  - Cell Separation/*methods
MH  - Intravital Microscopy/*methods
MH  - Mice
MH  - Molecular Biology/methods
MH  - Neurons/*physiology
MH  - Optical Imaging/*methods
MH  - Staining and Labeling/methods
EDAT- 2018/05/12 06:00
MHDA- 2019/04/16 06:00
CRDT- 2018/05/12 06:00
PHST- 2018/05/12 06:00 [entrez]
PHST- 2018/05/12 06:00 [pubmed]
PHST- 2019/04/16 06:00 [medline]
AID - nprot.2018.026 [pii]
AID - 10.1038/nprot.2018.026 [doi]
PST - ppublish
SO  - Nat Protoc. 2018 Jun;13(6):1275-1293. doi: 10.1038/nprot.2018.026. Epub 2018 May 
      10.