PMID- 29749273
OWN - NLM
STAT- MEDLINE
DCOM- 20190605
LR  - 20190605
IS  - 2169-141X (Electronic)
IS  - 2169-1401 (Linking)
VI  - 46
IP  - sup2
DP  - 2018
TI  - Enhanced osteogenic activity with boron-doped nanohydroxyapatite-loaded 
      poly(butylene adipate-co-terephthalate) fibrous 3D matrix.
PG  - 790-799
LID - 10.1080/21691401.2018.1470522 [doi]
AB  - In this study, three dimensional (3D) poly(butylene adipate-co-terephthalate) 
      (PBAT) fibrous scaffolds with more than 90% porosity were fabricated via wet 
      electrospinning method. Amorphous hydroxyapatite (HAp) and boron (B) doped 
      hydroxyapatite (B-HAp) nanoparticles were produced by microwave-assisted 
      biomimetic precipitation and encapsulated into PBAT fibres with the ratio of 5% 
      (w/w) in order to enhance osteogenic activity of the scaffolds. Cell culture 
      studies were carried out with human bone marrow derived stem cells (hBMSCs) and 
      they showed that alkaline phosphatase (ALP) activity and the amounts of collagen 
      and calcium were higher on B containing PBAT (B-HAp-PBAT) scaffolds during the 
      28-day culture period than that of the PBAT scaffolds. Moreover, hBMSCs 
      cultivated on B-HAp-PBAT scaffolds showed significantly higher expression levels 
      of both early and late stage osteogenic genes e.g. ALP, collagen I (COL-I), 
      osteocalcin (OCN) and osteopontin (OPN) at day 28 than that of the PBAT 
      scaffolds. Scanning electron microscope (SEM) photographs and energy dispersive 
      X-ray (EDX) analysis indicated that hBMSCs produced high amounts of mineralized 
      extracellular matrix (ECM) mainly on the surface of the 3 D matrices. This study 
      demonstrates that boron-containing 3 D nanofibrous PBAT scaffolds with their 
      osteoinductive and osteoconductive properties can be used as alternative 
      constructs for bone tissue engineering.
FAU - Arslan, Aysu
AU  - Arslan A
AD  - a Department of Chemical Engineering , Hacettepe University , Ankara , Turkey.
FAU - Cakmak, Soner
AU  - Cakmak S
AD  - b Department of Environmental Engineering , Hacettepe University , Ankara , 
      Turkey.
FAU - Gumusderelioglu, Menemse
AU  - Gumusderelioglu M
AD  - a Department of Chemical Engineering , Hacettepe University , Ankara , Turkey.
LA  - eng
PT  - Journal Article
DEP - 20180511
PL  - England
TA  - Artif Cells Nanomed Biotechnol
JT  - Artificial cells, nanomedicine, and biotechnology
JID - 101594777
RN  - 0 (Polyesters)
RN  - 0 (poly(butylene adipate-co-butylene terephthalate))
RN  - 059QF0KO0R (Water)
RN  - 91D9GV0Z28 (Durapatite)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - N9E3X5056Q (Boron)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Boron/*chemistry
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Durapatite/*chemistry/*pharmacology
MH  - Electricity
MH  - Extracellular Matrix/drug effects/metabolism
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/drug effects
MH  - Nanoparticles/*chemistry
MH  - Osteogenesis/*drug effects
MH  - Polyesters/*chemistry
MH  - Porosity
MH  - Water/chemistry
OTO - NOTNLM
OT  - Poly(butylene adipate-co-terephthalate)
OT  - bone scaffold
OT  - boron
OT  - hydroxyapatite
OT  - wet electrospinning
EDAT- 2018/05/12 06:00
MHDA- 2019/06/06 06:00
CRDT- 2018/05/12 06:00
PHST- 2018/05/12 06:00 [pubmed]
PHST- 2019/06/06 06:00 [medline]
PHST- 2018/05/12 06:00 [entrez]
AID - 10.1080/21691401.2018.1470522 [doi]
PST - ppublish
SO  - Artif Cells Nanomed Biotechnol. 2018;46(sup2):790-799. doi: 
      10.1080/21691401.2018.1470522. Epub 2018 May 11.