PMID- 29750879
OWN - NLM
STAT- MEDLINE
DCOM- 20190121
LR  - 20190712
IS  - 1802-9973 (Electronic)
IS  - 0862-8408 (Linking)
VI  - 67
IP  - 4
DP  - 2018 Aug 16
TI  - DHA upregulates FADS2 expression in primary cortical astrocytes exposed to 
      vitamin A.
PG  - 663-668
AB  - The fads2 gene encoding delta6-desaturase, the rate-limiting enzyme of the LCPUFA 
      biosynthesis is expressed in astrocytes. Dietary fatty acids, which cross the 
      blood-brain barrier, may regulate the transcription of lipogenic enzymes through 
      activation of transcription factors such as peroxisome proliferator-activated 
      receptors (PPARs). The PPARs form the transcription complex with retinoid X 
      receptors (RXRs) that are activated by 9-cis retinoic acid, a metabolite of 
      vitamin A (VA). The study examines whether challenge of astrocytes with VA, prior 
      24-h treatment with palmitic acid (PA), alpha-linolenic acid (ALA) or 
      docosahexaenoic acid (DHA) has the effect on the FADS2 expression. RT-qPCR showed 
      that in astrocytes not challenged with VA, PA increased fads2 gene expression and 
      DHA decreased it. However, in VA-primed astrocytes, PA doubled the FADS2 mRNA 
      levels, while DHA increased fads2 gene expression, oppositely to non-primed 
      cells. Furthermore, similar changes were seen in VA-primed astrocytes with regard 
      to delta6-desaturase protein levels following PA and DHA treatment. ALA did not 
      have any effect on the FADS2 mRNA and protein levels in either VA-primed or 
      non-primed astrocytes. These findings indicate that in the presence of vitamin A, 
      DHA upregulates fads2 gene expression in astrocytes.
FAU - Dziedzic, B
AU  - Dziedzic B
AD  - Department of Cell-to-Cell Communication, Medical University of Lodz, Lodz, 
      Poland. barbara.dziedzic@umed.lodz.pl.
FAU - Bewicz-Binkowska, D
AU  - Bewicz-Binkowska D
FAU - Zgorzynska, E
AU  - Zgorzynska E
FAU - Stulczewski, D
AU  - Stulczewski D
FAU - Wieteska, L
AU  - Wieteska L
FAU - Kaza, B
AU  - Kaza B
FAU - Walczewska, A
AU  - Walczewska A
LA  - eng
PT  - Journal Article
DEP - 20180510
PL  - Czech Republic
TA  - Physiol Res
JT  - Physiological research
JID - 9112413
RN  - 11103-57-4 (Vitamin A)
RN  - 25167-62-8 (Docosahexaenoic Acids)
RN  - EC 1.14.19.1 (Stearoyl-CoA Desaturase)
RN  - EC 1.14.99.- (delta 6-desaturase, rat)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Astrocytes/drug effects/*metabolism
MH  - Cells, Cultured
MH  - Cerebral Cortex/drug effects/*metabolism
MH  - Docosahexaenoic Acids/*pharmacology
MH  - Gene Expression
MH  - Rats
MH  - Rats, Wistar
MH  - Stearoyl-CoA Desaturase/*biosynthesis/genetics
MH  - Up-Regulation/drug effects/*physiology
MH  - Vitamin A/*pharmacology
EDAT- 2018/05/12 06:00
MHDA- 2019/01/22 06:00
CRDT- 2018/05/12 06:00
PHST- 2018/05/12 06:00 [pubmed]
PHST- 2019/01/22 06:00 [medline]
PHST- 2018/05/12 06:00 [entrez]
AID - 933708 [pii]
AID - 10.33549/physiolres.933708 [doi]
PST - ppublish
SO  - Physiol Res. 2018 Aug 16;67(4):663-668. doi: 10.33549/physiolres.933708. Epub 
      2018 May 10.