PMID- 29750888
OWN - NLM
STAT- MEDLINE
DCOM- 20190121
LR  - 20211204
IS  - 1802-9973 (Electronic)
IS  - 0862-8408 (Linking)
VI  - 67
IP  - 4
DP  - 2018 Aug 16
TI  - Adiponectin enhances biological functions of vascular endothelial progenitor 
      cells through the mTOR-STAT3 signaling pathway.
PG  - 563-570
AB  - Adiponectin (APN), an adipose tissue-excreted adipokine, plays protective roles 
      in metabolic and cardiovascular diseases. In this study, the effects and 
      mechanisms of APN on biological functions of rat vascular endothelial progenitor 
      cells (VEPCs) were investigated in vitro. After administrating APN in rat VEPCs, 
      the proliferation was measured by methyl thiazolyl tetrazolium (MTT) method, the 
      apoptotic rate was test by Flow cytometry assay, mRNA expression of B-cell 
      lymphoma-2 (Bcl-2) and vascular endothelial growth factor (VEGF) was determined 
      by real-time reverse transcriptase polymerase chain reaction (RT-PCR), and 
      protein expression of mechanistic target of rapamycin (mTOR), signal transducer 
      and activator of transcription 3 (STAT3) and phospho-STAT3 (pSTAT3) was analyzed 
      by Western blot. It was suggested that APN promoted the optical density (OD) 
      value of VEPCs, enhanced mRNA expression of Bcl-2 and VEGF, and inhibited cell 
      apoptotic rate. Furthermore, protein expression of pSTAT3 was also increased in 
      the presence of APN. Moreover, APN changed-proliferation, apoptosis and VEGF 
      expression of VEPCs were partially suppressed after blocking the mTOR-STAT3 
      signaling pathway by the mTOR inhibitor XL388. It was indicated that APN promoted 
      biological functions of VEPCs through targeting the mTOR-STAT3 signaling pathway.
FAU - Dong, Xiaoying
AU  - Dong X
AD  - College of Yingdong Agricultural Science and Engineering, Shaoguan University, 
      Shaoguan, China. willertang@163.com.
FAU - Yan, Xia
AU  - Yan X
FAU - Zhang, Wei
AU  - Zhang W
FAU - Tang, Shengqiu
AU  - Tang S
LA  - eng
PT  - Journal Article
DEP - 20180510
PL  - Czech Republic
TA  - Physiol Res
JT  - Physiological research
JID - 9112413
RN  - 0 (Adiponectin)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (Stat3 protein, rat)
RN  - 0 (Sulfones)
RN  - 0 (XL388)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Adiponectin/*pharmacology
MH  - Animals
MH  - Cell Line
MH  - Cell Proliferation/drug effects/physiology
MH  - Endothelial Progenitor Cells/drug effects/*physiology
MH  - Endothelium, Vascular/drug effects/*metabolism
MH  - Rats
MH  - STAT3 Transcription Factor/antagonists & inhibitors/*biosynthesis
MH  - Signal Transduction/drug effects/*physiology
MH  - Sulfones/pharmacology
MH  - TOR Serine-Threonine Kinases/antagonists & inhibitors/*biosynthesis
EDAT- 2018/05/12 06:00
MHDA- 2019/01/22 06:00
CRDT- 2018/05/12 06:00
PHST- 2018/05/12 06:00 [pubmed]
PHST- 2019/01/22 06:00 [medline]
PHST- 2018/05/12 06:00 [entrez]
AID - 933738 [pii]
AID - 10.33549/physiolres.933738 [doi]
PST - ppublish
SO  - Physiol Res. 2018 Aug 16;67(4):563-570. doi: 10.33549/physiolres.933738. Epub 
      2018 May 10.