PMID- 29751078
OWN - NLM
STAT- MEDLINE
DCOM- 20181105
LR  - 20181126
IS  - 1873-6351 (Electronic)
IS  - 0278-6915 (Linking)
VI  - 119
DP  - 2018 Sep
TI  - Gynosaponin TN-1 producing from the enzymatic conversion of gypenoside XLVI by 
      naringinase and its cytotoxicity on hepatoma cell lines.
PG  - 161-168
LID - S0278-6915(18)30297-7 [pii]
LID - 10.1016/j.fct.2018.05.007 [doi]
AB  - Gypenoside XLVI (gyp XLVI) is one of the major dammarane-type triterpenoid 
      saponins from Gynostamma pentaphallum with glucosyls at C-3 and C-20 positions, 
      which may constrain its bioactivities. The enzymatic conversion of gyp XLVI by 
      naringinase, and the cytotoxicity of enzymolysis product on SMMC7721 and Bel7402 
      hepatoma cells were investigated. The results showed that gynosaponin TN-1 (gyp 
      TN-1) was produced from the enzymatic conversion of gyp XLVI by naringinase. The 
      optimum enzymolysis conditions were pH 4.2, 47.3  degrees C, and 16 h, with a yield of 
      73.44 +/- 6.52% for gyp TN-1. In addition, gyp TN-1 exhibited higher inhibitory 
      activities on SMMC7721 and Bel7402 hepatoma cells than gyp XLVI. Results from 
      methyl thiazolyl tetrazolium (MTT) assay and acridine orange (AO)/ethidium 
      bromide (EB) double staining were highly consistent. These results demonstrated 
      that enzymatic conversion could be a promising method for producing gyp TN-1 from 
      the biotransformation of gyp XLVI and the preparation of gyp TN-1 might provide a 
      reference for the acquisition of novel anticancer drugs.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Zheng, Yi
AU  - Zheng Y
AD  - College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, 
      Fujian, 350002, China; Key Laboratory of Xiamen City for Plant Introduction & 
      Quarantine and Plant Derived Product, Xiamen Overseas Chinese Subtropical Plant 
      Introduction Garden, Xiamen, Fujian 361002, China.
FAU - Zheng, Zhizhong
AU  - Zheng Z
AD  - Key Laboratory of Xiamen City for Plant Introduction & Quarantine and Plant 
      Derived Product, Xiamen Overseas Chinese Subtropical Plant Introduction Garden, 
      Xiamen, Fujian 361002, China; Key Laboratory of Fujian Province for Physiology 
      and Biochemistry of Subtropical Plant, Fujian Institute of Subtropical Botany, 
      Xiamen, Fujian, 361006, China.
FAU - Ming, Yanlin
AU  - Ming Y
AD  - College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, 
      Fujian, 350002, China; Key Laboratory of Xiamen City for Plant Introduction & 
      Quarantine and Plant Derived Product, Xiamen Overseas Chinese Subtropical Plant 
      Introduction Garden, Xiamen, Fujian 361002, China; Key Laboratory of Fujian 
      Province for Physiology and Biochemistry of Subtropical Plant, Fujian Institute 
      of Subtropical Botany, Xiamen, Fujian, 361006, China. Electronic address: 
      xmyanlin@gmail.com.
FAU - Lin, Mengshi
AU  - Lin M
AD  - Food Science Program, Division of Food System & Bioengineering, University of 
      Missouri, Columbia, MO 65211-5160, USA.
FAU - Chen, Lianghua
AU  - Chen L
AD  - College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, 
      Fujian, 350002, China; Key Laboratory of Fujian Province for Physiology and 
      Biochemistry of Subtropical Plant, Fujian Institute of Subtropical Botany, 
      Xiamen, Fujian, 361006, China.
FAU - Huang, Wen
AU  - Huang W
AD  - Key Laboratory of Fujian Province for Physiology and Biochemistry of Subtropical 
      Plant, Fujian Institute of Subtropical Botany, Xiamen, Fujian, 361006, China.
FAU - Xiao, Jianbo
AU  - Xiao J
AD  - College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, 
      Fujian, 350002, China. Electronic address: jianboxiao@yahoo.com.
FAU - Lin, Hetong
AU  - Lin H
AD  - College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, 
      Fujian, 350002, China. Electronic address: hetonglin@163.com.
LA  - eng
PT  - Journal Article
DEP - 20180508
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (Saponins)
RN  - 0 (Triterpenes)
RN  - 0 (gynosaponin TN-1)
RN  - 0 (gypenoside XLVI)
RN  - EC 3.2.1.- (naringinase)
RN  - EC 3.2.1.21 (beta-Glucosidase)
SB  - IM
MH  - Antineoplastic Agents, Phytogenic/chemistry/*pharmacology
MH  - Carcinoma, Hepatocellular
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Hepatocytes/drug effects
MH  - Humans
MH  - Liver Neoplasms
MH  - Multienzyme Complexes/*metabolism
MH  - Saponins/biosynthesis/*chemistry/metabolism
MH  - Triterpenes/chemistry/*metabolism
MH  - beta-Glucosidase/*metabolism
OTO - NOTNLM
OT  - Biotransformation
OT  - Enzymatic conversion
OT  - Gynosaponin TN-1
OT  - Gypenoside XLVI
OT  - Hepatoma cell lines
OT  - Naringinase
EDAT- 2018/05/12 06:00
MHDA- 2018/11/06 06:00
CRDT- 2018/05/12 06:00
PHST- 2017/12/21 00:00 [received]
PHST- 2018/05/03 00:00 [revised]
PHST- 2018/05/04 00:00 [accepted]
PHST- 2018/05/12 06:00 [pubmed]
PHST- 2018/11/06 06:00 [medline]
PHST- 2018/05/12 06:00 [entrez]
AID - S0278-6915(18)30297-7 [pii]
AID - 10.1016/j.fct.2018.05.007 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 2018 Sep;119:161-168. doi: 10.1016/j.fct.2018.05.007. Epub 
      2018 May 8.