PMID- 29751397
OWN - NLM
STAT- MEDLINE
DCOM- 20180726
LR  - 20181202
IS  - 1873-6424 (Electronic)
IS  - 0269-7491 (Linking)
VI  - 235
DP  - 2018 Apr
TI  - Microplastic accumulation patterns and transfer of benzo[a]pyrene to adult 
      zebrafish (Danio rerio) gills and zebrafish embryos.
PG  - 918-930
LID - S0269-7491(17)33680-1 [pii]
LID - 10.1016/j.envpol.2018.01.028 [doi]
AB  - Since only a few studies have investigated effects of microplastics (MPs) by 
      routes other than ingestion, this study was designed to analyze the accumulation 
      patterns and transfer of toxic substances associated with microplastic exposure 
      by simple attachment to (1) adult zebrafish (Danio rerio) gills and (2) zebrafish 
      embryos. Two sizes of fluorescently labelled polymers (1-5 and 10-20 mum) loaded 
      with the model polycyclic aromatic hydrocarbon (PAH) benzo[a]pyrene (BaP) were 
      used to analyze fate, accumulation and transfer of microplastic-associated 
      persistent organic pollutants (POPs) on gills and embryos. Results indicate that 
      microplastics did not permanently accumulate at high amounts in adult zebrafish 
      gills after 6 nor 24 h of incubation: Most particles only superficially adhered 
      to the mucus layer on the filaments, which is constantly being excreted. In 
      contrast, the smaller and heavier MPs (1-5 mum) accumulated in high numbers on the 
      surface of zebrafish egg chorions. In both exposure scenarios, transfer of BaP 
      could be visualized with fluorescence microscopy: A prominent BaP signal was 
      visible both in gill filaments and arches after 6 and 24 h incubation and in 
      zebrafish embryos after exposure to BaP-spiked microplastics. Furthermore, the 
      gill EROD (Ethoxyresorufin-O-deethylase) assay showed a clear trend to CYP 1A 
      (Cytochrom P450 1 A) induction via exposure to BaP-spiked microplastics. However, 
      BaP from spiked microplastics did not reach sufficiently high concentrations to 
      be able to induce morphological effects in the fish embryo toxicity test (FET). 
      In contrast, control exposure to waterborne BaP did induce effects in the FET. As 
      a conclusion, microplastics can also transfer POPs not only via ingestion, but 
      also by simple attachment to epithelia or via the water column. However, further 
      studies are needed to clarify if these interactions are of environmental concern 
      relative to waterborne exposure to toxic substances.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Batel, Annika
AU  - Batel A
AD  - Aquatic Ecology and Toxicology, University of Heidelberg, Centre for Organismal 
      Studies (COS), Im Neuenheimer Feld 504, D-69120 Heidelberg, Germany. Electronic 
      address: annika.batel@cos.uni-heidelberg.de.
FAU - Borchert, Flora
AU  - Borchert F
AD  - Aquatic Ecology and Toxicology, University of Heidelberg, Centre for Organismal 
      Studies (COS), Im Neuenheimer Feld 504, D-69120 Heidelberg, Germany.
FAU - Reinwald, Hannes
AU  - Reinwald H
AD  - Aquatic Ecology and Toxicology, University of Heidelberg, Centre for Organismal 
      Studies (COS), Im Neuenheimer Feld 504, D-69120 Heidelberg, Germany.
FAU - Erdinger, Lothar
AU  - Erdinger L
AD  - Department of Hygiene and Medical Microbiology, University of Heidelberg, Im 
      Neuenheimer Feld 324, D-69120 Heidelberg, Germany.
FAU - Braunbeck, Thomas
AU  - Braunbeck T
AD  - Aquatic Ecology and Toxicology, University of Heidelberg, Centre for Organismal 
      Studies (COS), Im Neuenheimer Feld 504, D-69120 Heidelberg, Germany.
LA  - eng
PT  - Journal Article
DEP - 20180221
PL  - England
TA  - Environ Pollut
JT  - Environmental pollution (Barking, Essex : 1987)
JID - 8804476
RN  - 0 (Plastics)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (Water Pollutants, Chemical)
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Animals
MH  - Benzo(a)pyrene/*metabolism/toxicity
MH  - Biological Assay
MH  - Cytochrome P-450 CYP1A1/metabolism
MH  - Embryo, Nonmammalian/metabolism
MH  - Gills/drug effects/metabolism
MH  - Liver/drug effects
MH  - Plastics/*metabolism
MH  - Polycyclic Aromatic Hydrocarbons/metabolism/toxicity
MH  - Water Pollutants, Chemical/*metabolism/toxicity
MH  - Zebrafish/embryology/metabolism
OTO - NOTNLM
OT  - Microplastics
OT  - POP transfer
OT  - Zebrafish embryos
OT  - Zebrafish gills
OT  - benzo[a]pyrene
EDAT- 2018/05/13 06:00
MHDA- 2018/07/27 06:00
CRDT- 2018/05/13 06:00
PHST- 2017/09/01 00:00 [received]
PHST- 2018/01/11 00:00 [revised]
PHST- 2018/01/11 00:00 [accepted]
PHST- 2018/05/13 06:00 [entrez]
PHST- 2018/05/13 06:00 [pubmed]
PHST- 2018/07/27 06:00 [medline]
AID - S0269-7491(17)33680-1 [pii]
AID - 10.1016/j.envpol.2018.01.028 [doi]
PST - ppublish
SO  - Environ Pollut. 2018 Apr;235:918-930. doi: 10.1016/j.envpol.2018.01.028. Epub 
      2018 Feb 21.