PMID- 29752487
OWN - NLM
STAT- MEDLINE
DCOM- 20190614
LR  - 20190614
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Print)
IS  - 0175-7598 (Linking)
VI  - 102
IP  - 14
DP  - 2018 Jul
TI  - Fatty acid synthesis pathway provides lipid precursors for rhamnolipid 
      biosynthesis in Burkholderia thailandensis E264.
PG  - 6163-6174
LID - 10.1007/s00253-018-9059-5 [doi]
AB  - Rhamnolipid production was monitored for a period of 216 h using different 
      substrates in Pseudomonas aeruginosa PAO1 and Burkholderia thailandensis E264 
      which showed comparable crude yields attained by both after 216 h. The crude 
      yield for P. aeruginosa, however, was significantly higher at the early stages of 
      fermentation (72 or 144 h). Additionally, P. aeruginosa produced rhamnolipid with 
      odd and even carbon chain lipid moieties using odd carbon chain fatty acid 
      substrates (up to 45.97 and 67.57%, respectively). In contrast, B. thailandensis 
      produced rhamnolipid with predominantly even carbon chain lipid moieties (up to 
      99.26). These results indicate the use of the fatty acid synthesis (FAS II) 
      pathway as the main source of lipid precursors in rhamnolipid biosynthesis by B. 
      thailandensis. Isotope tracing using 0.25% stearic acid - d (35) + 1% glycerol as 
      carbon substrate showed a single pattern of deuterium incorporation: with 
      predominantly less than 15 deuterium atoms incorporated into a single 
      Di-C(14)-C(14) rhamnolipid molecule. This further indicates that the FAS II 
      pathway is the main source of the lipid precursor in rhamnolipid biosynthesis by 
      B. thailandensis. The pathogenicity of these strains was also assessed, and 
      results showed that B. thailandensis is significantly less pathogenic than P. 
      aeruginosa with an LC50 at 24 h > 2500, approximately three logs higher than P. 
      aeruginosa using the Galleria mellonella larva model.
FAU - Irorere, Victor U
AU  - Irorere VU
AD  - School of Biomedical Sciences, Faculty of Life and Health Sciences, Ulster 
      University, Coleraine, Northern Ireland, BT52 1SA, UK.
FAU - Smyth, Thomas J
AU  - Smyth TJ
AD  - Department of Life Sciences, Institute of Technology Sligo, County Sligo, 
      Ireland.
FAU - Cobice, Diego
AU  - Cobice D
AD  - School of Biomedical Sciences, Faculty of Life and Health Sciences, Ulster 
      University, Coleraine, Northern Ireland, BT52 1SA, UK.
FAU - McClean, Stephen
AU  - McClean S
AD  - School of Biomedical Sciences, Faculty of Life and Health Sciences, Ulster 
      University, Coleraine, Northern Ireland, BT52 1SA, UK.
FAU - Marchant, Roger
AU  - Marchant R
AD  - School of Biomedical Sciences, Faculty of Life and Health Sciences, Ulster 
      University, Coleraine, Northern Ireland, BT52 1SA, UK.
FAU - Banat, Ibrahim M
AU  - Banat IM
AD  - School of Biomedical Sciences, Faculty of Life and Health Sciences, Ulster 
      University, Coleraine, Northern Ireland, BT52 1SA, UK. im.banat@ulster.ac.uk.
LA  - eng
PT  - Journal Article
DEP - 20180512
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Bacterial Proteins)
RN  - 0 (Fatty Acids)
RN  - 0 (Glycolipids)
RN  - 0 (Isotopes)
RN  - 0 (Lipids)
RN  - 0 (rhamnolipid)
SB  - IM
MH  - Bacterial Proteins/metabolism
MH  - *Biosynthetic Pathways
MH  - Burkholderia/*metabolism
MH  - Fatty Acids/*biosynthesis
MH  - Fermentation
MH  - Glycolipids/*biosynthesis
MH  - Isotopes
MH  - Lipid Metabolism
MH  - Lipids/chemistry
MH  - Pseudomonas aeruginosa/metabolism
PMC - PMC6013509
OTO - NOTNLM
OT  - B. thailandensis
OT  - Deuterium labelling
OT  - FAS II
OT  - P. aeruginosa
OT  - Rhamnolipid
OT  - beta-oxidation
COIS- ETHICAL STATEMENT: This article does not contain any studies with human 
      participants or animals performed by any of the authors. CONFLICT OF INTEREST: 
      The authors declare that they have no conflict of interest.
EDAT- 2018/05/13 06:00
MHDA- 2019/06/15 06:00
PMCR- 2018/05/12
CRDT- 2018/05/13 06:00
PHST- 2018/03/22 00:00 [received]
PHST- 2018/04/27 00:00 [accepted]
PHST- 2018/04/25 00:00 [revised]
PHST- 2018/05/13 06:00 [pubmed]
PHST- 2019/06/15 06:00 [medline]
PHST- 2018/05/13 06:00 [entrez]
PHST- 2018/05/12 00:00 [pmc-release]
AID - 10.1007/s00253-018-9059-5 [pii]
AID - 9059 [pii]
AID - 10.1007/s00253-018-9059-5 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2018 Jul;102(14):6163-6174. doi: 
      10.1007/s00253-018-9059-5. Epub 2018 May 12.