PMID- 29752599
OWN - NLM
STAT- MEDLINE
DCOM- 20190522
LR  - 20190522
IS  - 1879-1123 (Electronic)
IS  - 1044-0305 (Print)
IS  - 1044-0305 (Linking)
VI  - 29
IP  - 6
DP  - 2018 Jun
TI  - Assignment by Negative-Ion Electrospray Tandem Mass Spectrometry of the 
      Tetrasaccharide Backbones of Monosialylated Glycans Released from Bovine Brain 
      Gangliosides.
PG  - 1308-1318
LID - 10.1007/s13361-018-1944-8 [doi]
AB  - Gangliosides, as plasma membrane-associated sialylated glycolipids, are antigenic 
      structures and they serve as ligands for adhesion proteins of pathogens, for 
      toxins of bacteria, and for endogenous proteins of the host. The detectability by 
      carbohydrate-binding proteins of glycan antigens and ligands on glycolipids can 
      be influenced by the differing lipid moieties. To investigate glycan sequences of 
      gangliosides as recognition structures, we have underway a program of work to 
      develop a "gangliome" microarray consisting of isolated natural gangliosides and 
      neoglycolipids (NGLs) derived from glycans released from them, and each linked to 
      the same lipid molecule for arraying and comparative microarray binding analyses. 
      Here, in the first phase of our studies, we describe a strategy for 
      high-sensitivity assignment of the tetrasaccharide backbones and application to 
      identification of eight of monosialylated glycans released from bovine brain 
      gangliosides. This approach is based on negative-ion electrospray mass 
      spectrometry with collision-induced dissociation (ESI-CID-MS/MS) of the 
      desialylated glycans. Using this strategy, we have the data on backbone regions 
      of four minor components among the monosialo-ganglioside-derived glycans; these 
      are of the ganglio-, lacto-, and neolacto-series. Graphical abstract.
FAU - Chai, Wengang
AU  - Chai W
AUID- ORCID: 0000-0003-2977-5347
AD  - Glycosciences Laboratory, Department of Medicine, Imperial College London, 
      Hammersmith Campus, London, W12 0NN, UK. w.chai@imperial.ac.uk.
FAU - Zhang, Yibing
AU  - Zhang Y
AD  - Glycosciences Laboratory, Department of Medicine, Imperial College London, 
      Hammersmith Campus, London, W12 0NN, UK.
FAU - Mauri, Laura
AU  - Mauri L
AD  - Department of Medical Chemistry, Biochemistry and Biotechnology, Center of 
      Excellence on Neurodegenerative Diseases, Graduate School of Biochemical, 
      Nutritional and Metabolic Sciences, University of Milan, 20090, Segrate, Italy.
FAU - Ciampa, Maria G
AU  - Ciampa MG
AD  - Department of Medical Chemistry, Biochemistry and Biotechnology, Center of 
      Excellence on Neurodegenerative Diseases, Graduate School of Biochemical, 
      Nutritional and Metabolic Sciences, University of Milan, 20090, Segrate, Italy.
FAU - Mulloy, Barbara
AU  - Mulloy B
AD  - Glycosciences Laboratory, Department of Medicine, Imperial College London, 
      Hammersmith Campus, London, W12 0NN, UK.
FAU - Sonnino, Sandro
AU  - Sonnino S
AD  - Department of Medical Chemistry, Biochemistry and Biotechnology, Center of 
      Excellence on Neurodegenerative Diseases, Graduate School of Biochemical, 
      Nutritional and Metabolic Sciences, University of Milan, 20090, Segrate, Italy.
FAU - Feizi, Ten
AU  - Feizi T
AD  - Glycosciences Laboratory, Department of Medicine, Imperial College London, 
      Hammersmith Campus, London, W12 0NN, UK. t.feizi@imperial.ac.uk.
LA  - eng
GR  - 108430/Z/15/Z/Wellcome Trust/United Kingdom
PT  - Journal Article
DEP - 20180511
PL  - United States
TA  - J Am Soc Mass Spectrom
JT  - Journal of the American Society for Mass Spectrometry
JID - 9010412
RN  - 0 (Gangliosides)
RN  - 0 (Oligosaccharides)
RN  - 0 (Polysaccharides)
RN  - GZP2782OP0 (N-Acetylneuraminic Acid)
SB  - IM
MH  - Animals
MH  - *Brain Chemistry
MH  - Carbohydrate Sequence
MH  - Cattle
MH  - Gangliosides/analysis/*chemistry
MH  - N-Acetylneuraminic Acid/analysis/*chemistry
MH  - Oligosaccharides/analysis/*chemistry
MH  - Polysaccharides/analysis/chemistry
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
PMC - PMC6003998
OTO - NOTNLM
OT  - Backbone structures
OT  - Bovine brain
OT  - Gangliosides
OT  - Glycans
OT  - Negative-ion ESI-CID-MA/MS
OT  - Oligosaccharides
OT  - Sequence assignment
EDAT- 2018/05/13 06:00
MHDA- 2019/05/23 06:00
PMCR- 2018/05/11
CRDT- 2018/05/13 06:00
PHST- 2018/03/05 00:00 [received]
PHST- 2018/03/10 00:00 [accepted]
PHST- 2018/03/10 00:00 [revised]
PHST- 2018/05/13 06:00 [pubmed]
PHST- 2019/05/23 06:00 [medline]
PHST- 2018/05/13 06:00 [entrez]
PHST- 2018/05/11 00:00 [pmc-release]
AID - 10.1007/s13361-018-1944-8 [pii]
AID - 1944 [pii]
AID - 10.1007/s13361-018-1944-8 [doi]
PST - ppublish
SO  - J Am Soc Mass Spectrom. 2018 Jun;29(6):1308-1318. doi: 10.1007/s13361-018-1944-8. 
      Epub 2018 May 11.