PMID- 29753743
OWN - NLM
STAT- MEDLINE
DCOM- 20190513
LR  - 20190513
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 501
IP  - 3
DP  - 2018 Jun 27
TI  - JLP-JNK signaling protects cancer cells from reactive oxygen species-induced cell 
      death.
PG  - 724-730
LID - S0006-291X(18)31104-5 [pii]
LID - 10.1016/j.bbrc.2018.05.055 [doi]
AB  - Oxidative stress, which can be caused by an overproduction of reactive oxygen 
      species (ROS), often leads to cell death. In recent years, c-Jun NH(2)-terminal 
      kinase (JNK)-associated leucine zipper protein (JLP, also known as SPAG9 or 
      JIP4), a scaffold protein for JNK mitogen-activated protein kinase (MAPK) 
      signaling pathways, was found to serve as a novel biomarker for cancer. However, 
      although JNK MAPK pathways are reported to be activated in response to various 
      stimuli, including oxidative stress, whether JLP is involved in ROS signaling 
      remains unknown. In this study, we examined the role of JLP in hydrogen peroxide 
      (H(2)O(2))-induced cancer cell death, and found that JLP knockdown (KD) cells 
      exhibit a substantially enhanced cell death response, along with increased 
      intracellular ROS levels. This is the first demonstration of a protective role 
      for JLP in response to cell-death stimulation. We also found that the 
      H(2)O(2)-induced JNK activation was attenuated in JLP KD cancer cells. The 
      decreases in cell viability and JNK activation in the JLP KD cells were almost 
      completely reversed by expressing wild-type JLP, but not a mutant JLP lacking the 
      JNK-binding domain. These data collectively suggest that the JLP-JNK signaling 
      pathway counteracts ROS-induced cancer cell death.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Li, Rong
AU  - Li R
AD  - Division of Molecular Cell Signaling, Cancer Research Institute, Kanazawa 
      University, Kanazawa, Japan.
FAU - Gunarta, I Ketut
AU  - Gunarta IK
AD  - Division of Molecular Cell Signaling, Cancer Research Institute, Kanazawa 
      University, Kanazawa, Japan.
FAU - Suzuki, Ryusuke
AU  - Suzuki R
AD  - Division of Molecular Cell Signaling, Cancer Research Institute, Kanazawa 
      University, Kanazawa, Japan.
FAU - Boldbaatar, Jambaldorj
AU  - Boldbaatar J
AD  - Division of Molecular Cell Signaling, Cancer Research Institute, Kanazawa 
      University, Kanazawa, Japan.
FAU - Nakazato, Ryota
AU  - Nakazato R
AD  - Division of Molecular Cell Signaling, Cancer Research Institute, Kanazawa 
      University, Kanazawa, Japan.
FAU - Yuliana, Dewi
AU  - Yuliana D
AD  - Division of Molecular Cell Signaling, Cancer Research Institute, Kanazawa 
      University, Kanazawa, Japan.
FAU - Davaakhuu, Gantulga
AU  - Davaakhuu G
AD  - Laboratory of Molecular Biology, Institute of General and Experimental Biology, 
      Mongolian Academy of Sciences, Ulaanbaatar, Mongolia.
FAU - Oyunsuren, Tsendsuren
AU  - Oyunsuren T
AD  - Laboratory of Molecular Biology, Institute of General and Experimental Biology, 
      Mongolian Academy of Sciences, Ulaanbaatar, Mongolia.
FAU - Takamatsu, Nobuhiko
AU  - Takamatsu N
AD  - Laboratory of Molecular Biology, Department of Biosciences, School of Science, 
      Kitasato University, Sagamihara, Japan.
FAU - Kobayashi, Masahiko
AU  - Kobayashi M
AD  - Division of Molecular Genetics, Cancer Research Institute, Kanazawa University, 
      Kanazawa, Japan.
FAU - Hirao, Atsushi
AU  - Hirao A
AD  - Division of Molecular Genetics, Cancer Research Institute, Kanazawa University, 
      Kanazawa, Japan; WPI Nano Life Science Institute (WPI-Nano LSI), Kanazawa 
      University, Kanazawa, Japan.
FAU - Yoshioka, Katsuji
AU  - Yoshioka K
AD  - Division of Molecular Cell Signaling, Cancer Research Institute, Kanazawa 
      University, Kanazawa, Japan. Electronic address: katsuji@staff.kanazawa-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (SPAG9 protein, human)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/*metabolism
MH  - Cell Death
MH  - Cell Line, Tumor
MH  - Humans
MH  - Hydrogen Peroxide/metabolism
MH  - *MAP Kinase Signaling System
MH  - Neoplasms/*metabolism/pathology
MH  - *Oxidative Stress
MH  - Reactive Oxygen Species/*metabolism
OTO - NOTNLM
OT  - Cell death
OT  - JNK
OT  - Oxidative stress
OT  - ROS
OT  - Scaffold protein
EDAT- 2018/05/14 06:00
MHDA- 2019/05/14 06:00
CRDT- 2018/05/14 06:00
PHST- 2018/05/05 00:00 [received]
PHST- 2018/05/09 00:00 [accepted]
PHST- 2018/05/14 06:00 [pubmed]
PHST- 2019/05/14 06:00 [medline]
PHST- 2018/05/14 06:00 [entrez]
AID - S0006-291X(18)31104-5 [pii]
AID - 10.1016/j.bbrc.2018.05.055 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2018 Jun 27;501(3):724-730. doi: 
      10.1016/j.bbrc.2018.05.055.