PMID- 29755453
OWN - NLM
STAT- MEDLINE
DCOM- 20190613
LR  - 20190613
IS  - 1664-3224 (Print)
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 9
DP  - 2018
TI  - CLEC10A Is a Specific Marker for Human CD1c(+) Dendritic Cells and Enhances Their 
      Toll-Like Receptor 7/8-Induced Cytokine Secretion.
PG  - 744
LID - 10.3389/fimmu.2018.00744 [doi]
LID - 744
AB  - Dendritic cells (DCs) are major players for the induction of immune responses. 
      Apart from plasmacytoid DCs (pDCs), human DCs can be categorized into two types 
      of conventional DCs: CD141(+) DCs (cDC1) and CD1c(+) DCs (cDC2). Defining 
      uniquely expressed surface markers on human immune cells is not only important 
      for the identification of DC subpopulations but also a prerequisite for 
      harnessing the DC subset-specific potential in immunomodulatory approaches, such 
      as antibody-mediated antigen targeting. Although others identified CLEC9A as a 
      specific endocytic receptor for CD141(+) DCs, such a receptor for CD1c(+) DCs has 
      not been discovered, yet. By performing transcriptomic and flow cytometric 
      analyses on human DC subpopulations from different lymphohematopoietic tissues, 
      we identified CLEC10A (CD301, macrophage galactose-type C-type lectin) as a 
      specific marker for human CD1c(+) DCs. We further demonstrate that CLEC10A 
      rapidly internalizes into human CD1c(+) DCs upon binding of a monoclonal antibody 
      directed against CLEC10A. The binding of a CLEC10A-specific bivalent ligand (the 
      MUC-1 peptide glycosylated with N-acetylgalactosamine) is limited to CD1c(+) DCs 
      and enhances the cytokine secretion (namely TNFalpha, IL-8, and IL-10) induced by TLR 
      7/8 stimulation. Thus, CLEC10A represents not only a candidate to better define 
      CD1c(+) DCs-due to its high endocytic potential-CLEC10A also exhibits an 
      interesting candidate receptor for future antigen-targeting approaches.
FAU - Heger, Lukas
AU  - Heger L
AD  - Department of Dermatology, Laboratory of Dendritic Cell Biology, 
      Friedrich-Alexander Universitat Erlangen-Nurnberg (FAU), University Hospital 
      Erlangen, Erlangen, Germany.
FAU - Balk, Silke
AU  - Balk S
AD  - Department of Dermatology, Laboratory of Dendritic Cell Biology, 
      Friedrich-Alexander Universitat Erlangen-Nurnberg (FAU), University Hospital 
      Erlangen, Erlangen, Germany.
FAU - Luhr, Jennifer J
AU  - Luhr JJ
AD  - Department of Dermatology, Laboratory of Dendritic Cell Biology, 
      Friedrich-Alexander Universitat Erlangen-Nurnberg (FAU), University Hospital 
      Erlangen, Erlangen, Germany.
FAU - Heidkamp, Gordon F
AU  - Heidkamp GF
AD  - Department of Dermatology, Laboratory of Dendritic Cell Biology, 
      Friedrich-Alexander Universitat Erlangen-Nurnberg (FAU), University Hospital 
      Erlangen, Erlangen, Germany.
FAU - Lehmann, Christian H K
AU  - Lehmann CHK
AD  - Department of Dermatology, Laboratory of Dendritic Cell Biology, 
      Friedrich-Alexander Universitat Erlangen-Nurnberg (FAU), University Hospital 
      Erlangen, Erlangen, Germany.
FAU - Hatscher, Lukas
AU  - Hatscher L
AD  - Department of Dermatology, Laboratory of Dendritic Cell Biology, 
      Friedrich-Alexander Universitat Erlangen-Nurnberg (FAU), University Hospital 
      Erlangen, Erlangen, Germany.
FAU - Purbojo, Ariawan
AU  - Purbojo A
AD  - Department of Pediatric Cardiac Surgery, Friedrich-Alexander Universitat 
      Erlangen-Nurnberg (FAU), University Hospital Erlangen, Erlangen, Germany.
FAU - Hartmann, Arndt
AU  - Hartmann A
AD  - Department of Pathology, Friedrich-Alexander Universitat Erlangen-Nurnberg (FAU), 
      University Hospital Erlangen, Erlangen, Germany.
FAU - Garcia-Martin, Fayna
AU  - Garcia-Martin F
AD  - Graduate School of Life Science and Faculty of Advanced Life Science, Hokkaido 
      University, Sapporo, Japan.
FAU - Nishimura, Shin-Ichiro
AU  - Nishimura SI
AD  - Graduate School of Life Science and Faculty of Advanced Life Science, Hokkaido 
      University, Sapporo, Japan.
FAU - Cesnjevar, Robert
AU  - Cesnjevar R
AD  - Department of Pediatric Cardiac Surgery, Friedrich-Alexander Universitat 
      Erlangen-Nurnberg (FAU), University Hospital Erlangen, Erlangen, Germany.
FAU - Nimmerjahn, Falk
AU  - Nimmerjahn F
AD  - Department of Biology, Chair of Genetics, Friedrich-Alexander Universitat 
      Erlangen-Nurnberg (FAU), Erlangen, Germany.
FAU - Dudziak, Diana
AU  - Dudziak D
AD  - Department of Dermatology, Laboratory of Dendritic Cell Biology, 
      Friedrich-Alexander Universitat Erlangen-Nurnberg (FAU), University Hospital 
      Erlangen, Erlangen, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180427
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Antigens, CD1)
RN  - 0 (CD1C protein, human)
RN  - 0 (CLEC10A protein, human)
RN  - 0 (Cytokines)
RN  - 0 (Glycoproteins)
RN  - 0 (Lectins, C-Type)
RN  - 0 (MUC1 protein, human)
RN  - 0 (Mucin-1)
RN  - 0 (TLR7 protein, human)
RN  - 0 (TLR8 protein, human)
RN  - 0 (Toll-Like Receptor 7)
RN  - 0 (Toll-Like Receptor 8)
SB  - IM
MH  - Adult
MH  - Antigens, CD1/*immunology
MH  - Cytokines/immunology
MH  - Dendritic Cells/*immunology
MH  - Glycoproteins/*immunology
MH  - Humans
MH  - Lectins, C-Type/*immunology
MH  - Mucin-1/immunology
MH  - Toll-Like Receptor 7/immunology
MH  - Toll-Like Receptor 8/immunology
PMC - PMC5934495
OTO - NOTNLM
OT  - CD1c+ dendritic cells
OT  - CLEC10A
OT  - antigen targeting
OT  - cDC2
OT  - dendritic cell subpopulations
OT  - lineage marker
EDAT- 2018/05/15 06:00
MHDA- 2018/05/15 06:01
PMCR- 2018/01/01
CRDT- 2018/05/15 06:00
PHST- 2018/01/06 00:00 [received]
PHST- 2018/03/26 00:00 [accepted]
PHST- 2018/05/15 06:00 [entrez]
PHST- 2018/05/15 06:00 [pubmed]
PHST- 2018/05/15 06:01 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2018.00744 [doi]
PST - epublish
SO  - Front Immunol. 2018 Apr 27;9:744. doi: 10.3389/fimmu.2018.00744. eCollection 
      2018.