PMID- 29758489
OWN - NLM
STAT- MEDLINE
DCOM- 20181012
LR  - 20211204
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 60
DP  - 2018 Jul
TI  - Fisetin inhibits the generation of inflammatory mediators in 
      interleukin-1beta-induced human lung epithelial cells by suppressing the NF-kappaB and 
      ERK1/2 pathways.
PG  - 202-210
LID - S1567-5769(18)30215-7 [pii]
LID - 10.1016/j.intimp.2018.05.004 [doi]
AB  - Fisetin, a flavone that can be isolated from fruits and vegetables, has 
      anti-tumor and anti-oxidative properties and ameliorates airway 
      hyperresponsiveness in asthmatic mice. This study investigated whether fisetin 
      can suppress the expression of inflammatory mediators and intercellular adhesion 
      molecule 1 (ICAM-1) in A549 human lung epithelial cells that were stimulated with 
      interleukin-1beta (IL-1beta) to induce inflammatory responses. A549 cells were treated 
      with fisetin (3-30 muM) and then with IL-1beta. Fisetin significantly inhibited COX-2 
      expression and reduced prostaglandin E(2) production, and it suppressed the 
      levels of IL-8, CCL5, monocyte chemotactic protein 1, tumor necrosis factor alpha, 
      and IL-6. Fisetin also significantly attenuated the expression of chemokine and 
      inflammatory cytokine genes and decreased the expression of ICAM-1, which 
      mediates THP-1 monocyte adhesion to inflammatory A549 cells. Fisetin decreased 
      the translocation of nuclear transcription factor kappa-B (NF-kappaB) subunit p65 
      into the nucleus and inhibited the phosphorylation of proteins in the ERK1/2 
      pathway. Co-treatment of IL-1beta-stimulated A549 cells with ERK1/2 inhibitors plus 
      fisetin reduced ICAM-1 expression. Furthermore, fisetin significantly increased 
      the effects of the protective antioxidant pathway by promoting the expression of 
      nuclear factor erythroid-2-related factor-2 and heme oxygenase 1. Taken together, 
      these data suggest that fisetin has anti-inflammatory effects and that it 
      suppresses the expression of chemokines, inflammatory cytokines, and ICAM-1 by 
      suppressing the NF-kappaB and ERK1/2 signaling pathways in IL-1beta-stimulated human 
      lung epithelial A549 cells.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Peng, Hui-Ling
AU  - Peng HL
AD  - Graduate Institute of Health Industry Technology, Research Center for Food and 
      Cosmetic Safety, Research Center for Chinese Herbal Medicine, College of Human 
      Ecology, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., 
      Guishan Dist., Taoyuan City 33303, Taiwan.
FAU - Huang, Wen-Chung
AU  - Huang WC
AD  - Graduate Institute of Health Industry Technology, Research Center for Food and 
      Cosmetic Safety, Research Center for Chinese Herbal Medicine, College of Human 
      Ecology, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., 
      Guishan Dist., Taoyuan City 33303, Taiwan; Division of Allergy, Asthma, and 
      Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, Linkou, 
      Guishan Dist., Taoyuan City 33303, Taiwan. Electronic address: 
      wchuang@mail.cgust.edu.tw.
FAU - Cheng, Shu-Chen
AU  - Cheng SC
AD  - Department of Traditional Chinese Medicine, Chang Gung Memorial Hospital, 
      Taoyuan, Taiwan; Graduate Institute of Clinical Medical Sciences, College of 
      Medicine, Chang Gung University, Taoyuan, Taiwan.
FAU - Liou, Chian-Jiun
AU  - Liou CJ
AD  - Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang 
      Gung Memorial Hospital, Linkou, Guishan Dist., Taoyuan City 33303, Taiwan; 
      Department of Nursing, Division of Basic Medical Sciences, Research Center for 
      Chinese Herbal Medicine, Chang Gung University of Science and Technology, No.261, 
      Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan. Electronic address: 
      ccliu@mail.cgust.edu.tw.
LA  - eng
PT  - Journal Article
DEP - 20180512
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (Flavonoids)
RN  - 0 (Flavonols)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NF-kappa B)
RN  - 0 (NFE2L2 protein, human)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - EC 1.14.14.18 (HMOX1 protein, human)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 1.14.99.1 (PTGS2 protein, human)
RN  - OO2ABO9578 (fisetin)
SB  - IM
MH  - A549 Cells
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Cell Line
MH  - Cyclooxygenase 2/genetics/metabolism
MH  - Cytokines/*metabolism
MH  - Epithelial Cells/*drug effects/metabolism
MH  - Flavonoids/*pharmacology
MH  - Flavonols
MH  - Heme Oxygenase-1/metabolism
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/genetics/metabolism
MH  - Lung/cytology
MH  - MAP Kinase Signaling System/*drug effects
MH  - NF-E2-Related Factor 2/metabolism
MH  - NF-kappa B/*antagonists & inhibitors/metabolism
MH  - Signal Transduction/drug effects
OTO - NOTNLM
OT  - Anti-inflammation
OT  - COX-2
OT  - ERK1/2
OT  - Fisetin
OT  - ICAM-1
OT  - NF-kappaB
EDAT- 2018/05/15 06:00
MHDA- 2018/10/13 06:00
CRDT- 2018/05/15 06:00
PHST- 2018/01/17 00:00 [received]
PHST- 2018/05/04 00:00 [revised]
PHST- 2018/05/08 00:00 [accepted]
PHST- 2018/05/15 06:00 [pubmed]
PHST- 2018/10/13 06:00 [medline]
PHST- 2018/05/15 06:00 [entrez]
AID - S1567-5769(18)30215-7 [pii]
AID - 10.1016/j.intimp.2018.05.004 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2018 Jul;60:202-210. doi: 10.1016/j.intimp.2018.05.004. Epub 
      2018 May 12.