PMID- 29758985
OWN - NLM
STAT- MEDLINE
DCOM- 20180611
LR  - 20180611
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 66
IP  - 22
DP  - 2018 Jun 6
TI  - Ultrasonic Pretreatment Combined with Dry-State Glycation Reduced the 
      Immunoglobulin E/Immunoglobulin G-Binding Ability of alpha-Lactalbumin Revealed by 
      High-Resolution Mass Spectrometry.
PG  - 5691-5698
LID - 10.1021/acs.jafc.8b00489 [doi]
AB  - Bovine alpha-lactalbumin (alpha-LA) is one of major food allergens in cow's milk. The 
      present work sought to research the effects of ultrasonic pretreatment combined 
      with dry heating-induced glycation between alpha-LA and galactose on the 
      immunoglobulin E (IgE)/immunoglobulin G (IgG)-binding ability and glycation 
      extent of alpha-LA, determined by inhibition enzyme-linked immunosorbent assay and 
      high-resolution mass spectrometry, respectively. The IgE/IgG-binding ability of 
      glycated alpha-LA was significantly decreased as a result of ultrasonic pretreatment, 
      while the average molecular weight, incorporation ratio (IR) value, location and 
      number of glycation sites, and degree of substitution per peptide (DSP) value 
      were elevated. When the mixtures of alpha-LA and galactose were pretreated by 
      ultrasonication at 150 W/cm(2), glycated alpha-LA possesses seven glycation sites, 
      the highest IR and DSP values, and the lowest IgE/IgG-binding ability. Therefore, 
      the decrease in the IgE/IgG-binding ability of alpha-LA depends upon not only the 
      shielding effect of the linear epitope found to be caused by the glycation of 
      K13, K16, K58, K93, and K98 sites but also the intensified glycation extent, 
      which reflected in the increase of the IR value, the number of glycation sites, 
      and the DSP value. Moreover, allergenic proteins and monosaccharides pretreated 
      by ultrasonication and then followed by dry-state glycation were revealed as a 
      promising way of achieving lower allergenicity of proteins in food processing.
FAU - Liu, Jun
AU  - Liu J
AD  - College of Life Sciences , Jiangxi Normal University , Nanchang , Jiangxi 330022 
      , People's Republic of China.
FAU - Tu, Zong-Cai
AU  - Tu ZC
AUID- ORCID: 0000-0001-8877-4681
AD  - College of Life Sciences , Jiangxi Normal University , Nanchang , Jiangxi 330022 
      , People's Republic of China.
AD  - State Key Laboratory of Food Science and Technology , Nanchang University , 
      Nanchang , Jiangxi 330047 , People's Republic of China.
FAU - Liu, Guang-Xian
AU  - Liu GX
AD  - College of Life Sciences , Jiangxi Normal University , Nanchang , Jiangxi 330022 
      , People's Republic of China.
FAU - Niu, Chen-di
AU  - Niu CD
AD  - Department of Chemistry , University of Massachusetts Amherst , Amherst , 
      Massachusetts 01003 , United States.
FAU - Yao, Hong-Lin
AU  - Yao HL
AD  - Department of Chemistry , University of Massachusetts Amherst , Amherst , 
      Massachusetts 01003 , United States.
FAU - Wang, Hui
AU  - Wang H
AUID- ORCID: 0000-0002-5538-1838
AD  - State Key Laboratory of Food Science and Technology , Nanchang University , 
      Nanchang , Jiangxi 330047 , People's Republic of China.
FAU - Sha, Xiao-Mei
AU  - Sha XM
AD  - College of Life Sciences , Jiangxi Normal University , Nanchang , Jiangxi 330022 
      , People's Republic of China.
FAU - Shao, Yan-Hong
AU  - Shao YH
AD  - College of Life Sciences , Jiangxi Normal University , Nanchang , Jiangxi 330022 
      , People's Republic of China.
FAU - Kaltashov, Igor A
AU  - Kaltashov IA
AUID- ORCID: 0000-0002-4355-6039
AD  - Department of Chemistry , University of Massachusetts Amherst , Amherst , 
      Massachusetts 01003 , United States.
LA  - eng
PT  - Journal Article
DEP - 20180523
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9013-90-5 (Lactalbumin)
SB  - IM
MH  - Animals
MH  - Glycosylation
MH  - Immunoglobulin E/*chemistry/immunology
MH  - Immunoglobulin G/*chemistry/immunology
MH  - Lactalbumin/*chemistry/immunology
MH  - Mass Spectrometry
MH  - Milk/chemistry/immunology
MH  - Ultrasonics
OTO - NOTNLM
OT  - IgE/IgG-binding ability
OT  - glycation
OT  - mass spectrometry
OT  - ultrasonication
OT  - alpha-lactalbumin
EDAT- 2018/05/16 06:00
MHDA- 2018/06/12 06:00
CRDT- 2018/05/16 06:00
PHST- 2018/05/16 06:00 [pubmed]
PHST- 2018/06/12 06:00 [medline]
PHST- 2018/05/16 06:00 [entrez]
AID - 10.1021/acs.jafc.8b00489 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2018 Jun 6;66(22):5691-5698. doi: 10.1021/acs.jafc.8b00489. 
      Epub 2018 May 23.