PMID- 29760727
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2018
DP  - 2018
TI  - Expanded CD133(+) Cells from Human Umbilical Cord Blood Improved Heart Function 
      in Rats after Severe Myocardial Infarction.
PG  - 5412478
LID - 10.1155/2018/5412478 [doi]
LID - 5412478
AB  - Pharmacological approaches are partially effective in limiting infarct size. Cell 
      therapies using a cell population enriched with endothelial progenitor cells 
      (EPCs) CD133(+) have opened new perspectives for the treatment of ischemic areas 
      after infarction. This preclinical study evaluated the effect of intramyocardial 
      transplantation of purified or expanded human umbilical cord blood-derived 
      CD133(+) cells on the recovery of rats following acute myocardial infarction 
      (AMI). Histology studies, electrocardiogram, and fluorescence in situ 
      hybridization (FISH) were used to evaluate heart recovery. Purified CD133(+) 
      cells, enriched in endothelial progenitor cells, when expanded in vitro acquired 
      an endothelial-like cell phenotype expressing CD31 and von Willebrand factor 
      (vWF). The group of infarcted rats that received expanded CD133(+) cells had a 
      more significant recovery of contraction performance and less heart remodeling 
      than the group that received purified CD133(+) cells. Either purified or expanded 
      CD133(+) cells were able to induce neovascularization in the infarcted myocardium 
      in an equivalent manner. Few human cells were detected in the infarcted 
      myocardium of the rats 28 days after transplantation suggesting that the effects 
      observed might be related primarily to paracrine activity. Although both cell 
      populations ameliorated the infarcted heart and are suitable for regeneration of 
      the vascular system, expanded CD133(+) cells are more beneficial and promising 
      candidates for vascular regeneration.
FAU - Correa, Alejandro
AU  - Correa A
AUID- ORCID: 0000-0002-5887-1453
AD  - Carlos Chagas Institute, Oswaldo Cruz Foundation, FIOCRUZ, Curitiba, PR, Brazil.
FAU - Ottoboni, Gabriel Salles
AU  - Ottoboni GS
AD  - Core for Cell Technology, Pontificia Universidade Catolica do Parana, Curitiba, 
      PR, Brazil.
FAU - Senegaglia, Alexandra Cristina
AU  - Senegaglia AC
AD  - Core for Cell Technology, Pontificia Universidade Catolica do Parana, Curitiba, 
      PR, Brazil.
FAU - Capriglione, Luiz Guilherme Achcar
AU  - Capriglione LGA
AD  - Core for Cell Technology, Pontificia Universidade Catolica do Parana, Curitiba, 
      PR, Brazil.
FAU - Miyague, Nelson Itiro
AU  - Miyague NI
AD  - Core for Cell Technology, Pontificia Universidade Catolica do Parana, Curitiba, 
      PR, Brazil.
FAU - Leite, Lidiane Maria Boldrini
AU  - Leite LMB
AD  - Core for Cell Technology, Pontificia Universidade Catolica do Parana, Curitiba, 
      PR, Brazil.
FAU - Jamur, Valderez Ravaglio
AU  - Jamur VR
AD  - Core for Cell Technology, Pontificia Universidade Catolica do Parana, Curitiba, 
      PR, Brazil.
FAU - Rebelatto, Carmen Lucia Kuniyoshi
AU  - Rebelatto CLK
AD  - Core for Cell Technology, Pontificia Universidade Catolica do Parana, Curitiba, 
      PR, Brazil.
FAU - Olandoski, Marcia
AU  - Olandoski M
AD  - Core for Cell Technology, Pontificia Universidade Catolica do Parana, Curitiba, 
      PR, Brazil.
FAU - Brofman, Paulo Roberto Slud
AU  - Brofman PRS
AD  - Core for Cell Technology, Pontificia Universidade Catolica do Parana, Curitiba, 
      PR, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20180411
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC5925035
EDAT- 2018/05/16 06:00
MHDA- 2018/05/16 06:01
PMCR- 2018/04/11
CRDT- 2018/05/16 06:00
PHST- 2017/07/04 00:00 [received]
PHST- 2017/11/08 00:00 [revised]
PHST- 2017/12/12 00:00 [accepted]
PHST- 2018/05/16 06:00 [entrez]
PHST- 2018/05/16 06:00 [pubmed]
PHST- 2018/05/16 06:01 [medline]
PHST- 2018/04/11 00:00 [pmc-release]
AID - 10.1155/2018/5412478 [doi]
PST - epublish
SO  - Stem Cells Int. 2018 Apr 11;2018:5412478. doi: 10.1155/2018/5412478. eCollection 
      2018.