PMID- 29762523
OWN - NLM
STAT- MEDLINE
DCOM- 20180921
LR  - 20240318
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 19
IP  - 5
DP  - 2018 May 15
TI  - Silver Nanoparticles: Two-Faced Neuronal Differentiation-Inducing Material in 
      Neuroblastoma (SH-SY5Y) Cells.
LID - 10.3390/ijms19051470 [doi]
LID - 1470
AB  - We have previously demonstrated the potential of biologically synthesized silver 
      nanoparticles (AgNP) in the induction of neuronal differentiation of human 
      neuroblastoma, SH-SY5Y cells; we aimed herein to unveil its molecular mechanism 
      in comparison to the well-known neuronal differentiation-inducing agent, 
      all-trans-retinoic acid (RA). AgNP-treated SH-SY5Y cells showed significantly 
      higher reactive oxygen species (ROS) generation, stronger mitochondrial membrane 
      depolarization, lower dual-specificity phosphatase expression, higher 
      extracellular-signal-regulated kinase (ERK) phosphorylation, lower AKT 
      phosphorylation, and lower expression of the genes encoding the antioxidant 
      enzymes than RA-treated cells. Notably, pretreatment with N-acetyl-l-cysteine 
      significantly abolished AgNP-induced neuronal differentiation, but not in that 
      induced by RA. ERK inhibition, but not AKT inhibition, suppresses neurite growth 
      that is induced by AgNP. Taken together, our results uncover the pivotal 
      contribution of ROS in the AgNP-induced neuronal differentiation mechanism, which 
      is different from that of RA. However, the negative consequence of AgNP-induced 
      neurite growth may be high ROS generation and the downregulation of the 
      expression of the genes encoding the antioxidant enzymes, which prompts the 
      future consideration and an in-depth study of the application of 
      AgNP-differentiated cells in neurodegenerative disease therapy.
FAU - Abdal Dayem, Ahmed
AU  - Abdal Dayem A
AD  - Department of Stem Cell and Regenerative Biotechnology, Incurable Disease Animal 
      Model & Stem Cell Institute (IDASI), Konkuk University, Seoul 05029, Korea. 
      ahmed_morsy86@yahoo.com.
FAU - Lee, Soo Bin
AU  - Lee SB
AD  - Department of Stem Cell and Regenerative Biotechnology, Incurable Disease Animal 
      Model & Stem Cell Institute (IDASI), Konkuk University, Seoul 05029, Korea. 
      soobineey@naver.com.
FAU - Choi, Hye Yeon
AU  - Choi HY
AD  - Department of Stem Cell and Regenerative Biotechnology, Incurable Disease Animal 
      Model & Stem Cell Institute (IDASI), Konkuk University, Seoul 05029, Korea. 
      hyeon.choi24@gmail.com.
FAU - Cho, Ssang-Goo
AU  - Cho SG
AUID- ORCID: 0000-0002-0968-7932
AD  - Department of Stem Cell and Regenerative Biotechnology, Incurable Disease Animal 
      Model & Stem Cell Institute (IDASI), Konkuk University, Seoul 05029, Korea. 
      ssangoo@konkuk.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20180515
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Reactive Oxygen Species)
RN  - 3M4G523W1G (Silver)
RN  - 5688UTC01R (Tretinoin)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/pharmacology
MH  - Cell Line, Tumor
MH  - Humans
MH  - Membrane Potential, Mitochondrial
MH  - Metal Nanoparticles/*chemistry
MH  - Mitogen-Activated Protein Kinase 3/metabolism
MH  - Neuroblastoma/*metabolism
MH  - *Neurogenesis
MH  - Neurons/cytology/*drug effects/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Silver/chemistry
MH  - Tretinoin/pharmacology
PMC - PMC5983825
OTO - NOTNLM
OT  - differentiation
OT  - kinase
OT  - mitochondria
OT  - neuroblastoma
OT  - phosphatase
OT  - reactive oxygen species
OT  - silver nanoparticles
COIS- The authors declare no conflicts of interest.
EDAT- 2018/05/16 06:00
MHDA- 2018/09/22 06:00
PMCR- 2018/05/01
CRDT- 2018/05/16 06:00
PHST- 2018/04/16 00:00 [received]
PHST- 2018/05/08 00:00 [revised]
PHST- 2018/05/11 00:00 [accepted]
PHST- 2018/05/16 06:00 [entrez]
PHST- 2018/05/16 06:00 [pubmed]
PHST- 2018/09/22 06:00 [medline]
PHST- 2018/05/01 00:00 [pmc-release]
AID - ijms19051470 [pii]
AID - ijms-19-01470 [pii]
AID - 10.3390/ijms19051470 [doi]
PST - epublish
SO  - Int J Mol Sci. 2018 May 15;19(5):1470. doi: 10.3390/ijms19051470.