PMID- 29765079
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20190919
LR  - 20190919
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 May 15
TI  - Determination of flumequine enantiomers and 7-hydroxyflumequine in water and 
      sediment by chiral HPLC coupled with hybrid quadrupole-time-of-flight mass 
      spectrometer.
PG  - 7582
LID - 10.1038/s41598-018-25889-5 [doi]
LID - 7582
AB  - A liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for 
      simultaneous enantiomeric analysis of flumequine and its metabolite 
      7-hydroxyflumequine in water and sediment had been developed based on the 
      separation method. Sediment samples were extracted with ACN and EDTA-Mcllvaine 
      buffer solution (40:60, v/v) then were enriched and cleaned-up by Cleanert PEP 
      solid-phase extraction cartridges. The extract solvent, solid cartridges, mobile 
      phase ratios, and chiral separation column were all optimized to reach high 
      sensitivity and selectivity, good peak shape, and satisfactory resolution. The 
      results showed that the calibration curves of flumequine enantiomers and 
      7-hydroxyflumequine were linear in the range of 1.0 to 200.0 microg/L with 
      correlation coefficients of 0.9822-0.9988, the mean recoveries for both the 
      enantiomers ranged from 69.9-84.6% with relative standard deviations (RSDs) being 
      13.1% or below. The limits of detection (LODs) for both flumequine enantiomers 
      were 2.5 microg/L and 5.0 microg/kg in water and sediment samples, whereas the limits of 
      quantification (LOQs) were 8.0 microg/L and 15.0 microg/kg, respectively. While the LODs 
      for 7-hydroxyflumequine were 3.2 microg/L in water samples and 7.0 microg/kg in sediment 
      samples. The proposed method will be extended for studies on the degradation 
      kinetics and environmental behaviors and providing additional information for 
      reliable risk assessment of these chiral antibiotics.
FAU - Xue, Moyong
AU  - Xue M
AD  - Feed Research Institute, Chinese Academy of Agricultural Science, Beijing, 
      100081, China.
AD  - Institute of Food and Nutrition Development, Ministry of Agriculture, Beijing, 
      10081, China.
FAU - Qin, Yuchang
AU  - Qin Y
AD  - Institute of Animal Science, Chinese Academy of Agriculture Sciences, Beijing, 
      100193, China. qinyuchang@caas.cn.
FAU - Gu, Xu
AU  - Gu X
AD  - Feed Research Institute, Chinese Academy of Agricultural Science, Beijing, 
      100081, China. guxu@caas.cn.
FAU - Li, Junguo
AU  - Li J
AD  - Feed Research Institute, Chinese Academy of Agricultural Science, Beijing, 
      100081, China.
FAU - Gao, Yunfeng
AU  - Gao Y
AD  - Feed Research Institute, Chinese Academy of Agricultural Science, Beijing, 
      100081, China.
FAU - Yang, Xiaowei
AU  - Yang X
AD  - Supervision Institute of Veterinary Medicine and Feed of Tianjin, Tianjin, 
      300402, China.
FAU - Yao, Ting
AU  - Yao T
AD  - Beijing Institute of Feed Control, Beijing, 100012, China.
FAU - Zhao, Zhen
AU  - Zhao Z
AD  - Feed Research Institute, Chinese Academy of Agricultural Science, Beijing, 
      100081, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180515
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
PMC - PMC5953928
COIS- The authors declare no competing interests.
EDAT- 2018/05/17 06:00
MHDA- 2018/05/17 06:01
PMCR- 2018/05/15
CRDT- 2018/05/17 06:00
PHST- 2017/12/07 00:00 [received]
PHST- 2018/04/30 00:00 [accepted]
PHST- 2018/05/17 06:00 [entrez]
PHST- 2018/05/17 06:00 [pubmed]
PHST- 2018/05/17 06:01 [medline]
PHST- 2018/05/15 00:00 [pmc-release]
AID - 10.1038/s41598-018-25889-5 [pii]
AID - 25889 [pii]
AID - 10.1038/s41598-018-25889-5 [doi]
PST - epublish
SO  - Sci Rep. 2018 May 15;8(1):7582. doi: 10.1038/s41598-018-25889-5.