PMID- 29768138
OWN - NLM
STAT- MEDLINE
DCOM- 20200622
LR  - 20200622
IS  - 1208-6002 (Electronic)
IS  - 0829-8211 (Linking)
VI  - 96
IP  - 6
DP  - 2018 Dec
TI  - Knockdown of the prolyl isomerase Pin1 inhibits Hep-2 cell growth, migration, and 
      invasion by targeting the beta-catenin signaling pathway.
PG  - 734-741
LID - 10.1139/bcb-2017-0334 [doi]
AB  - There is increasing evidence indicating that peptidylprolyl cis/trans isomerase, 
      NIMA-interacting 1 (Pin1) plays a decisive role in a variety of cancers. 
      Nevertheless, its function in laryngeal squamous cell carcinoma (LSCC) has not 
      been elaborated. The aim of this study is to determine the role of Pin1 in LSCC. 
      Here, we established stably transfected Hep-2 cells with low expression of Pin1. 
      Intriguingly, cell proliferation, migration, and invasion was significantly 
      inhibited in Pin1-silenced Hep-2 cells. Similarly, knockdown of Pin1 induced 
      apoptosis of Hep-2 cells, as evidenced by increased expression of 
      cleaved-caspase-3, cleaved-PARP, and bax, and decreased expression of bcl2. We 
      also demonstrated that silencing of Pin1 down-regulated beta-catenin and cyclin D1 
      expression. Inversely, over-expression of beta-catenin reversed the inhibiting 
      effect of Pin1 silencing on Hep-2 cells. Moreover, we proved that knockdown of 
      Pin1 inhibited tumorigenesis of Hep-2 cells in vivo. Taken together, we 
      demonstrate that silencing of Pin1 effectively suppresses the growth of Hep-2 
      cells through beta-catenin, indicating that Pin1 possess the potential to serve as a 
      therapeutic target for the treatment of LSCC.
FAU - Fan, Guoliang
AU  - Fan G
AD  - Department of Otolaryngology, Harbin First Hospital, Harbin, People's Republic of 
      China.
FAU - Wang, Lin
AU  - Wang L
AD  - Department of Otolaryngology, Harbin First Hospital, Harbin, People's Republic of 
      China.
FAU - Xu, Jia
AU  - Xu J
AD  - Department of Otolaryngology, Harbin First Hospital, Harbin, People's Republic of 
      China.
FAU - Jiang, Ping
AU  - Jiang P
AD  - Department of Pathology, Harbin First Hospital, Harbin, People's Republic of 
      China.
FAU - Wang, Wei
AU  - Wang W
AD  - Department of Otolaryngology, Harbin First Hospital, Harbin, People's Republic of 
      China.
FAU - Huang, Ying
AU  - Huang Y
AD  - Department of Pathology, Harbin First Hospital, Harbin, People's Republic of 
      China.
FAU - Lv, Minggang
AU  - Lv M
AD  - Department of Otolaryngology, Harbin First Hospital, Harbin, People's Republic of 
      China.
FAU - Liu, Shaoting
AU  - Liu S
AD  - Department of Otolaryngology, Harbin First Hospital, Harbin, People's Republic of 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180516
PL  - Canada
TA  - Biochem Cell Biol
JT  - Biochemistry and cell biology = Biochimie et biologie cellulaire
JID - 8606068
RN  - 0 (CTNNB1 protein, human)
RN  - 0 (NIMA-Interacting Peptidylprolyl Isomerase)
RN  - 0 (beta Catenin)
RN  - EC 5.2.1.8 (PIN1 protein, human)
SB  - IM
MH  - Cell Cycle/genetics
MH  - Cell Proliferation/*genetics
MH  - Down-Regulation/genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Gene Knockdown Techniques/methods
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics
MH  - NIMA-Interacting Peptidylprolyl Isomerase/*genetics
MH  - beta Catenin/*metabolism
OTO - NOTNLM
OT  - Pin1
OT  - apoptose
OT  - apoptosis
OT  - cancer larynge
OT  - laryngeal cancer
OT  - proliferation
OT  - proliferation
OT  - beta-catenin
OT  - beta-catenine
EDAT- 2018/05/17 06:00
MHDA- 2020/06/23 06:00
CRDT- 2018/05/17 06:00
PHST- 2018/05/17 06:00 [pubmed]
PHST- 2020/06/23 06:00 [medline]
PHST- 2018/05/17 06:00 [entrez]
AID - 10.1139/bcb-2017-0334 [doi]
PST - ppublish
SO  - Biochem Cell Biol. 2018 Dec;96(6):734-741. doi: 10.1139/bcb-2017-0334. Epub 2018 
      May 16.