PMID- 29772166
OWN - NLM
STAT- MEDLINE
DCOM- 20190321
LR  - 20190321
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 90
IP  - 11
DP  - 2018 Jun 5
TI  - Pixel Counting of Fluorescence Spots Triggered by DNA Walkers for Ultrasensitive 
      Quantification of Nucleic Acid.
PG  - 6357-6361
LID - 10.1021/acs.analchem.8b01146 [doi]
AB  - A pixel counting strategy is designed on the basis of DNA walker-triggered 
      fluorescence spots for ultrasensitive detection of nucleic acid. The 
      two-dimensional DNA walker was constructed by hybridization of two types of 
      capture DNAs, which were covalently modified by click chemistry on a glass slide, 
      and dye-labeled hairpin structure (hDNA) as track and swing strand (sDNA) as 
      DNAzyme, respectively. Introduction of target DNA unlocked the sDNA via strand 
      displacement to form the activated DNAzyme, and the latter cut nearby hDNA with 
      Mn(2+) as cofactor, resulting in fluorescence recovery of dye-labeled hDNA on the 
      substrate due to the separation from the quencher. Meanwhile, the DNAzyme 
      sequence of sDNA was released to cut the next hDNA and, thus, initiated 
      autonomous walking of sDNA for signal amplification. The enhanced fluorescence 
      spots were digitalized as pixels on the basis of DNA walker-built compartments 
      and extracted by a homemade program in MATLAB. The association between 
      fluorescent pixel numbers and DNA concentrations was further proved by a 
      mathematical model and led to an ultrasensitive quantification of nucleic acid 
      with a linear range from 100 fM to 10 pM. The designed pixel counting strategy 
      shows a more sensitive and accurate comparison with conventional methods based on 
      fluorescence intensity or spot counts and provides a new dimension in designing 
      next-type biosensors.
FAU - Zhu, Longyi
AU  - Zhu L
AUID- ORCID: 0000-0002-0898-4804
AD  - State Key Laboratory of Analytical Chemistry for Life Science, School of 
      Chemistry and Chemical Engineering , Nanjing University , Nanjing , Jiangsu 
      210023 , P. R. China.
FAU - Liu, Qihui
AU  - Liu Q
AD  - State Key Laboratory of Analytical Chemistry for Life Science, School of 
      Chemistry and Chemical Engineering , Nanjing University , Nanjing , Jiangsu 
      210023 , P. R. China.
FAU - Yang, Bangyu
AU  - Yang B
AD  - State Key Laboratory of Analytical Chemistry for Life Science, School of 
      Chemistry and Chemical Engineering , Nanjing University , Nanjing , Jiangsu 
      210023 , P. R. China.
FAU - Ju, Huangxian
AU  - Ju H
AUID- ORCID: 0000-0002-6741-5302
AD  - State Key Laboratory of Analytical Chemistry for Life Science, School of 
      Chemistry and Chemical Engineering , Nanjing University , Nanjing , Jiangsu 
      210023 , P. R. China.
FAU - Lei, Jianping
AU  - Lei J
AUID- ORCID: 0000-0002-3594-180X
AD  - State Key Laboratory of Analytical Chemistry for Life Science, School of 
      Chemistry and Chemical Engineering , Nanjing University , Nanjing , Jiangsu 
      210023 , P. R. China.
LA  - eng
PT  - Letter
PT  - Research Support, Non-U.S. Gov't
DEP - 20180523
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Catalytic)
RN  - 9007-49-2 (DNA)
RN  - I38ZP9992A (Magnesium)
SB  - IM
MH  - Biosensing Techniques/methods
MH  - Click Chemistry
MH  - DNA/*analysis
MH  - DNA Probes/*chemistry
MH  - DNA, Catalytic/*chemistry
MH  - Limit of Detection
MH  - Magnesium/chemistry
MH  - Microscopy, Fluorescence/methods
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - Nucleic Acid Conformation
MH  - Spectrometry, Fluorescence/methods
EDAT- 2018/05/18 06:00
MHDA- 2019/03/22 06:00
CRDT- 2018/05/18 06:00
PHST- 2018/05/18 06:00 [pubmed]
PHST- 2019/03/22 06:00 [medline]
PHST- 2018/05/18 06:00 [entrez]
AID - 10.1021/acs.analchem.8b01146 [doi]
PST - ppublish
SO  - Anal Chem. 2018 Jun 5;90(11):6357-6361. doi: 10.1021/acs.analchem.8b01146. Epub 
      2018 May 23.