PMID- 29773098
OWN - NLM
STAT- MEDLINE
DCOM- 20181127
LR  - 20181127
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 34
IP  - 3
DP  - 2018 Mar
TI  - [Polydatin inhibits cell proliferation and expressions of inflammatory cytokines 
      in THP-1 cells induced by ox-LDL via up-regulating SIRT1].
PG  - 193-198
AB  - Objective To investigate the effects of polydatin on cell proliferation and 
      cytokine expression in THP-1 monocyte-derived macrophages (MDMs) induced by 
      oxidized low-density lipoprotein (ox-LDL), and the possible mechanisms. Methods 
      MDMs were divided into the control group (only treated with ordinary culture 
      medium), ox-LDL group (treated wtih 80 mumol/L ox-LDL for 24 hours), polydatin 
      treatment group (treated with 100 mumol/L polydatin for 2 hours prior to the 
      treatment with 80 mumol/L ox-LDL for 24 hours) and EX-527 treatment group (treated 
      with 10 mumol/L SIRT1 inhibitor EX-527 for 2 hours prior to the treatment with 
      ox-LDL and polydatin). The effects of polydatin on ox-LDL-induced oxidative 
      proliferation and cytokine expression in MDMs were evaluated by CCK-8 assay. 
      Spectrofluorometry was used to determine the intracellular level of superoxide 
      dismutase (SOD) and malondialdehyde (MDA). DCFH-DA loading was used to detect the 
      content of reactive oxidative species (ROS). The levels of silent mating type 
      information regulator 1 (SIRT1), monocyte chemoattractant protein-1 (MCP-1), 
      tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) were examined by 
      real-time quantitative PCR and Western blotting. Results Polydatin (100 mumol/L) 
      inhibited the proliferation of MDMs induced by ox-LDL, decreased the levels of 
      MDA and ROS, whereas the level of SOD increased. The mRNA and protein levels of 
      SIRT1 in MDMs were inhibited by ox-LDL, whereas the expressions of MCP-1, TNF-alpha 
      and IL-6 were promoted. Pre-treatment with EX-527 attenuated the inhibitory 
      effects of polydatin on the proliferation of MDMs, inhibited the expressions of 
      SIRT1, promoted the expressions of MCP-1, TNF-alpha and IL-6. Conclusion Polydatin 
      up-regulates the expression of SIRT1 to increase the ability of anti-macrophage 
      proliferation, reduce the level of the intracellular ROS induced by ox-LDL, and 
      inhibit the expression of inflammatory cytokines.
FAU - Ma, Yi
AU  - Ma Y
AD  - Department of Cardiology, Affiliated Hospital, Zunyi Medical University, Zunyi 
      563000, China. *Corresponding author, E-mail: mayimayizmc@126.com.
FAU - Ruan, Yunjun
AU  - Ruan Y
AD  - Department of Cardiology, Guangzhou Military General Hospital, Guangzhou 510010, 
      China.
FAU - Wang, Yuyan
AU  - Wang Y
AD  - Deartment of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 
      510515, China.
FAU - Wu, Saizhu
AU  - Wu S
AD  - Deartment of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 
      510515, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Glucosides)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Stilbenes)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (oxidized low density lipoprotein)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 3.5.1.- (SIRT1 protein, human)
RN  - EC 3.5.1.- (Sirtuin 1)
RN  - XM261C37CQ (polydatin)
SB  - IM
MH  - Cell Proliferation/*drug effects
MH  - Chemokine CCL2/genetics/metabolism
MH  - Cytokines/genetics/*metabolism
MH  - Glucosides/*pharmacology
MH  - Humans
MH  - Interleukin-6/genetics/metabolism
MH  - Lipoproteins, LDL/*pharmacology
MH  - Macrophages/cytology/*drug effects/metabolism
MH  - Malondialdehyde/metabolism
MH  - Oxidative Stress/drug effects
MH  - Reactive Oxygen Species/metabolism
MH  - Sirtuin 1/genetics/*metabolism
MH  - Stilbenes/*pharmacology
MH  - Superoxide Dismutase/metabolism
MH  - THP-1 Cells
MH  - Tumor Necrosis Factor-alpha/genetics/metabolism
MH  - Up-Regulation
EDAT- 2018/05/19 06:00
MHDA- 2018/11/28 06:00
CRDT- 2018/05/19 06:00
PHST- 2018/05/19 06:00 [entrez]
PHST- 2018/05/19 06:00 [pubmed]
PHST- 2018/11/28 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2018 Mar;34(3):193-198.