PMID- 29774144
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2049-9434 (Print)
IS  - 2049-9442 (Electronic)
IS  - 2049-9434 (Linking)
VI  - 8
IP  - 6
DP  - 2018 Jun
TI  - Effects of passage and cryopreservation on neurotrophic factor secretion from 
      choroid plexus epithelial cells.
PG  - 535-539
LID - 10.3892/br.2018.1087 [doi]
AB  - The aim of the present study was to evaluate the effects of passage and 
      cryopreservation of choroid plexus epithelial cells on their secretion of 
      neurotrophic factors. Choroid plexus epithelial cells were cryopreserved and 
      thawed following primary culture or passage cultured for up to two passages. The 
      supernatant of primary, first/second passage and cryopreserved-thawed choroid 
      plexus epithelial cells was collected when cells reached 80-90% confluence. ELISA 
      was used to quantify brain-derived neurotrophic factor (BDNF), glial cell-derived 
      neurotrophic factor (GDNF), nerve growth factor (NGF) and ciliary neurotrophic 
      factor (CNTF) levels in the cell supernatant. First passage and 
      cryopreserved-thawed cells secreted less BDNF and CNTF compared with primary 
      cultured cells and increased levels of these two factors compared with second 
      passage cells, and increased levels of GDNF and NGF compared with primary 
      cultured and second passage cells (all P<0.05). Therefore, first passage culture 
      decreased BDNF and CNTF secretion but increased NGF and GDNF compared with 
      primary culture; second passage culture diminished neurotrophic factor secretion 
      compared with first passage culture; and cryopreservation did not weaken the 
      function of choroid plexus epithelial cells in secreting BDNF, GDNF, NGF and 
      CNTF. The current study demonstrates that first passage and cryopreserved-thawed 
      choroid plexus epithelial cells have an enhanced function to secrete neurotrophic 
      factors including BDNF, GDNF, NGF and CNTF.
FAU - Zhao, Feng
AU  - Zhao F
AD  - Department of Thoracic Surgery, The Second Affiliated Hospital, School of 
      Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China.
FAU - Ding, Xiao-Yan
AU  - Ding XY
AD  - Department of Ophthalmology, Xi'an No. 3 Hospital, Xi'an, Shaanxi 710018, P.R. 
      China.
FAU - Wu, Feng
AU  - Wu F
AD  - Department of Pathogenic Biology and Immunology, School of Medicine, 
      Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
FAU - Li, Xiao-Hui
AU  - Li XH
AD  - Department of Radiology, The Second Affiliated Hospital, School of Medicine, 
      Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China.
FAU - Li, Yu-Huan
AU  - Li YH
AD  - Department of Orthopedics, The Second Affiliated Hospital, School of Medicine, 
      Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China.
FAU - Huang, Sheng-Li
AU  - Huang SL
AD  - Department of Orthopedics, The Second Affiliated Hospital, School of Medicine, 
      Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180419
PL  - England
TA  - Biomed Rep
JT  - Biomedical reports
JID - 101613227
PMC - PMC5954601
OTO - NOTNLM
OT  - choroid plexus epithelial cells
OT  - cryopreservation
OT  - neurotrophic factors
OT  - passage culture
EDAT- 2018/05/19 06:00
MHDA- 2018/05/19 06:01
PMCR- 2018/04/19
CRDT- 2018/05/19 06:00
PHST- 2018/01/02 00:00 [received]
PHST- 2018/04/04 00:00 [accepted]
PHST- 2018/05/19 06:00 [entrez]
PHST- 2018/05/19 06:00 [pubmed]
PHST- 2018/05/19 06:01 [medline]
PHST- 2018/04/19 00:00 [pmc-release]
AID - BR-0-0-1087 [pii]
AID - 10.3892/br.2018.1087 [doi]
PST - ppublish
SO  - Biomed Rep. 2018 Jun;8(6):535-539. doi: 10.3892/br.2018.1087. Epub 2018 Apr 19.