PMID- 29774421
OWN - NLM
STAT- MEDLINE
DCOM- 20190401
LR  - 20190401
IS  - 1436-5073 (Electronic)
IS  - 0026-3672 (Linking)
VI  - 185
IP  - 6
DP  - 2018 May 17
TI  - A fluorometric aptasensor for methamphetamine based on fluorescence resonance 
      energy transfer using cobalt oxyhydroxide nanosheets and carbon dots.
PG  - 303
LID - 10.1007/s00604-018-2842-2 [doi]
AB  - Cobalt oxyhydroxide (CoOOH) nanosheets are efficient fluorescence quenchers due 
      to their specific optical properties and high surface area. The combination of 
      CoOOH nanosheets and carbon dots (CDs) has not been used in any aptasensor based 
      on fluorescence quenching so far. An aptamer based fluorometric assay is 
      introduced that is making use of fluorescent CDs conjugated to the aptamer 
      against methamphetamine (MTA), and of CoOOH nanosheets which reduce the 
      fluorescence of the CDs as a quencher. The results revealed that the conjugated 
      CDs with aptamers were able to enclose the CoOOH nanosheets. Consequently, 
      fluorescence is quenched. If the aptamer on the CD binds MTA, the CDs are 
      detached from CoOOH nanosheets. As a result, fluorescence is restored 
      proportionally to zhe MTA concentration. The fluorometric limit of detection is 
      1 nM with a dynamic range from 5 to 156 nM. The method was validated by comparing 
      the results obtained by the new method to those obtained by ion mobility 
      spectroscopy. Theoretical studies showed that the distance between CoOOH 
      nanosheet and C-Ds is approximately 7.6 A which can illustrate the possibility of 
      FRET phenomenon. The interactions of MTA and the aptamer were investigated using 
      molecular dynamic simulation (MDS). Graphical abstract Carbon dots (C-Ds) were 
      prepared from grape leaves, conjugated to aptamer, and adsorbed on CoOOH 
      nanosheets. So, the fluorescence of C-Ds is quenched. On addition of MTA, 
      fluorescence is restored.
FAU - Saberi, Zeinab
AU  - Saberi Z
AD  - Department of Chemistry, Isfahan University of Technology, Isfahan, 84156-83111, 
      Islamic Republic of Iran.
FAU - Rezaei, Behzad
AU  - Rezaei B
AD  - Department of Chemistry, Isfahan University of Technology, Isfahan, 84156-83111, 
      Islamic Republic of Iran. rezaei@cc.iut.ac.ir.
FAU - Faroukhpour, Hossein
AU  - Faroukhpour H
AD  - Department of Chemistry, Isfahan University of Technology, Isfahan, 84156-83111, 
      Islamic Republic of Iran.
FAU - Ensafi, Ali Ashghar
AU  - Ensafi AA
AD  - Department of Chemistry, Isfahan University of Technology, Isfahan, 84156-83111, 
      Islamic Republic of Iran.
LA  - eng
PT  - Journal Article
DEP - 20180517
PL  - Austria
TA  - Mikrochim Acta
JT  - Mikrochimica acta
JID - 7808782
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Oxides)
RN  - 0HWN445Z2E (HCoO2)
RN  - 3G0H8C9362 (Cobalt)
RN  - 44RAL3456C (Methamphetamine)
RN  - 7440-44-0 (Carbon)
SB  - IM
MH  - Aptamers, Nucleotide/chemistry/genetics/*metabolism
MH  - Carbon/*chemistry
MH  - Cobalt/*chemistry
MH  - *Fluorescence Resonance Energy Transfer
MH  - Humans
MH  - Methamphetamine/*analysis/blood
MH  - Models, Molecular
MH  - Nucleic Acid Conformation
MH  - Oxides/*chemistry
MH  - Quantum Dots/*chemistry
OTO - NOTNLM
OT  - Bioassay
OT  - FRET
OT  - Fluorophore
OT  - Theoretical studies
OT  - Transition metal dichalcogenides
EDAT- 2018/05/19 06:00
MHDA- 2019/04/02 06:00
CRDT- 2018/05/19 06:00
PHST- 2018/02/21 00:00 [received]
PHST- 2018/05/10 00:00 [accepted]
PHST- 2018/05/19 06:00 [entrez]
PHST- 2018/05/19 06:00 [pubmed]
PHST- 2019/04/02 06:00 [medline]
AID - 10.1007/s00604-018-2842-2 [pii]
AID - 10.1007/s00604-018-2842-2 [doi]
PST - epublish
SO  - Mikrochim Acta. 2018 May 17;185(6):303. doi: 10.1007/s00604-018-2842-2.