PMID- 29775609
OWN - NLM
STAT- MEDLINE
DCOM- 20190513
LR  - 20211204
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 501
IP  - 3
DP  - 2018 Jun 27
TI  - Kruppel-like factor 4 (KLF-4) plays a crucial role in simvastatin (SVT)-induced 
      differentiation of rabbit articular chondrocytes.
PG  - 814-819
LID - S0006-291X(18)31157-4 [pii]
LID - 10.1016/j.bbrc.2018.05.094 [doi]
AB  - Simvastatin is a cholesterol-lowing reagent that is derived synthetically from 
      the fermentation of Aspergillus terreus. Recently, SVT has been shown to possess 
      a protective effect of chondrocytes. Kruppel-like factor 4 (KLF-4) is a zinc 
      finger transcription factor that plays crucial roles during the development and 
      maintenance of multiple organs. However, the roles of KLF-4 in chondrocytes have 
      not been well unknown. Here, we investigated whether KLF-4 regulates SVT-caused 
      differentiated phenotype of chondrocytes. A KLF-4 cDNA or KLF-4 siRNA was 
      transfected into SVT-treated chondrocytes. Western blot analysis, RT-PCR and 
      immunofluorescence staining analyzed expression of type II collagen and SOX-9, 
      marker proteins of differentiation. The results showed overexpression of KLF-4 
      accelerates SVT-induced type II collagen expression, as determined by western 
      blot analysis and causes sulfated-proteoglycan synthesis, as detected by Alcian 
      blue staining. RT-PCR revealed that ectopic expression of KLF-4 induces 
      SVT-caused SOX-9, a transcription factor of type II collagen, expression. 
      Transfection of KLF-4 siRNA reversed SVT-caused type II collagen and SOX-9 
      expression and inhibited SVT-induced sulfated proteoglycan production. This study 
      indicates that KLF-4 plays critical role in SVT-caused chondrocytes 
      differentiation.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Yu, Seon-Mi
AU  - Yu SM
AD  - Department of Biological Sciences, College of Natural Sciences, Kongju National 
      University, Gongju, Republic of Korea.
FAU - Kim, Song Ja
AU  - Kim SJ
AD  - Department of Biological Sciences, College of Natural Sciences, Kongju National 
      University, Gongju, Republic of Korea. Electronic address: ksj85@kongju.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180518
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Anticholesteremic Agents)
RN  - 0 (Kruppel-Like Factor 4)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (SOX9 Transcription Factor)
RN  - AGG2FN16EV (Simvastatin)
SB  - IM
MH  - Animals
MH  - Anticholesteremic Agents/*pharmacology
MH  - Cartilage, Articular/cytology/drug effects/metabolism
MH  - Cell Differentiation/*drug effects
MH  - Cells, Cultured
MH  - Chondrocytes/cytology/*drug effects/metabolism
MH  - Kruppel-Like Factor 4
MH  - Kruppel-Like Transcription Factors/analysis/*metabolism
MH  - Rabbits
MH  - SOX9 Transcription Factor/analysis/metabolism
MH  - Simvastatin/*pharmacology
OTO - NOTNLM
OT  - Chondrocytes
OT  - Differentiation
OT  - Kruppel-like factor 4
OT  - Simvastatin
EDAT- 2018/05/19 06:00
MHDA- 2019/05/14 06:00
CRDT- 2018/05/19 06:00
PHST- 2018/05/11 00:00 [received]
PHST- 2018/05/14 00:00 [accepted]
PHST- 2018/05/19 06:00 [pubmed]
PHST- 2019/05/14 06:00 [medline]
PHST- 2018/05/19 06:00 [entrez]
AID - S0006-291X(18)31157-4 [pii]
AID - 10.1016/j.bbrc.2018.05.094 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2018 Jun 27;501(3):814-819. doi: 
      10.1016/j.bbrc.2018.05.094. Epub 2018 May 18.