PMID- 29775893 OWN - NLM STAT- MEDLINE DCOM- 20181010 LR - 20181010 IS - 1950-6007 (Electronic) IS - 0753-3322 (Linking) VI - 104 DP - 2018 Aug TI - Puerarin prevents vascular endothelial injury through suppression of NF-kappaB activation in LPS-challenged human umbilical vein endothelial cells. PG - 261-267 LID - S0753-3322(18)31032-1 [pii] LID - 10.1016/j.biopha.2018.05.038 [doi] AB - OBJECTIVE: In the present study, we aimed to explore the effects of puerarin on vascular endothelial cell injury induced by lipopolysaccharide (LPS) and its underlying mechanisms. METHODS: The cell viability and morphological changes were assessed using the cell counting kit-8 (CCK-8) assay and 4 ,6-diamidino-2-phenylindole (DAPI) staining, respectively. The levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), monocyte/macrophage chemotactic protein-1 (MCP-1), IL-8, intercellular cell adhesion molecule-1 (ICAM-1), thrombomodulin (TM) and plasminogen activator inhibitor-1 (PAI-1) in cell culture supernatant were determined by the enzyme-linked immunosorbent assay (ELISA). The neutrophils adhesion to endothelial cells were examined by myeloperoxidase activity assay. The nuclear translocation of nuclear factor-kappaB p65 (NF-kappaB p65) was assessed by immunofluorescence analysis. RESULTS: Compared with the control group, LPS challenge significantly injured human umbilical vein endothelial cells (HUVECs) and increased the levels of TNF-alpha, IL-1beta, MCP-1, IL-8, ICAM-1, TM and PAI-1 in the cell culture supernatants. The neutrophils adhesion to endothelial cells were significantly increased in LPS-challenged HUVECs. Moreover, LPS challenge increased the nuclear translocation of NF-kappaB p65. However, puerarin pre-treatment attenuated the vascular endothelial injury and reduced the levels of TNF-alpha, IL-1beta, MCP-1, IL-8, ICAM-1, TM and PAI-1 in cell supernatants of LPS-challenged HUVECs. In addition, the neutrophils adhesion to HUVECs induced by LPS were also decreased by puerarin pre-treatment. Furthermore, puerarin pre-treatment reduced the nuclear translocation of NF-kappaB p65 elicited by LPS. CONCLUSIONS: Puerarin prevented LPS-induced vascular endothelial injury, the mechanism of which might be related to the suppression of NF-kappaB activation and subsequently altered levels of inflammatory factors and coagulation-related factors. CI - Copyright (c) 2018 Elsevier Masson SAS. All rights reserved. FAU - Deng, Hua-Fei AU - Deng HF AD - Department of Pathophysiology, School of Basic Medical Science, Xiangnan University, Chenzhou, Hunan, 423000, PR China; Department of Pathophysiology, School of Basic Medical Science, Xiangya School of Medicine, Central South University, Changsha, Hunan, 410078, PR China. Electronic address: denghuafei@126.com. FAU - Wang, Sha AU - Wang S AD - Department of Pathophysiology, School of Basic Medical Science, Xiangnan University, Chenzhou, Hunan, 423000, PR China. FAU - Li, Lian AU - Li L AD - Department of Pathophysiology, School of Basic Medical Science, Xiangnan University, Chenzhou, Hunan, 423000, PR China. FAU - Zhou, Qin AU - Zhou Q AD - Department of Pathophysiology, School of Basic Medical Science, Xiangnan University, Chenzhou, Hunan, 423000, PR China. FAU - Guo, Wan-Bei AU - Guo WB AD - Department of Pathophysiology, School of Basic Medical Science, Xiangnan University, Chenzhou, Hunan, 423000, PR China. FAU - Wang, Xiao-Li AU - Wang XL AD - Department of Pathophysiology, School of Basic Medical Science, Xiangya School of Medicine, Central South University, Changsha, Hunan, 410078, PR China. FAU - Liu, Mei-Dong AU - Liu MD AD - Department of Pathophysiology, School of Basic Medical Science, Xiangya School of Medicine, Central South University, Changsha, Hunan, 410078, PR China. FAU - Liu, Ke AU - Liu K AD - Department of Pathophysiology, School of Basic Medical Science, Xiangya School of Medicine, Central South University, Changsha, Hunan, 410078, PR China. FAU - Xiao, Xian-Zhong AU - Xiao XZ AD - Department of Pathophysiology, School of Basic Medical Science, Xiangya School of Medicine, Central South University, Changsha, Hunan, 410078, PR China. Electronic address: xiaoxianzhong@csu.edu.cn. LA - eng PT - Journal Article DEP - 20180525 PL - France TA - Biomed Pharmacother JT - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JID - 8213295 RN - 0 (Chemokine CCL2) RN - 0 (Interleukin-1beta) RN - 0 (Interleukin-8) RN - 0 (Isoflavones) RN - 0 (Lipopolysaccharides) RN - 0 (NF-kappa B) RN - 0 (Plasminogen Activator Inhibitor 1) RN - 0 (Tumor Necrosis Factor-alpha) RN - 126547-89-5 (Intercellular Adhesion Molecule-1) RN - Z9W8997416 (puerarin) SB - IM MH - Cell Survival/drug effects MH - Cells, Cultured MH - Chemokine CCL2/metabolism MH - Gene Expression Regulation/drug effects MH - Human Umbilical Vein Endothelial Cells/*drug effects/metabolism MH - Humans MH - Intercellular Adhesion Molecule-1/metabolism MH - Interleukin-1beta/metabolism MH - Interleukin-8/metabolism MH - Isoflavones/*pharmacology MH - Lipopolysaccharides/*pharmacology MH - NF-kappa B/*metabolism MH - Plasminogen Activator Inhibitor 1/metabolism MH - Signal Transduction/drug effects MH - Tumor Necrosis Factor-alpha/metabolism OTO - NOTNLM OT - Endothelial cells OT - Injury OT - Lipopolysaccharide OT - NF-kappaB OT - Puerarin EDAT- 2018/05/19 06:00 MHDA- 2018/10/12 06:00 CRDT- 2018/05/19 06:00 PHST- 2018/02/12 00:00 [received] PHST- 2018/05/05 00:00 [revised] PHST- 2018/05/08 00:00 [accepted] PHST- 2018/05/19 06:00 [pubmed] PHST- 2018/10/12 06:00 [medline] PHST- 2018/05/19 06:00 [entrez] AID - S0753-3322(18)31032-1 [pii] AID - 10.1016/j.biopha.2018.05.038 [doi] PST - ppublish SO - Biomed Pharmacother. 2018 Aug;104:261-267. doi: 10.1016/j.biopha.2018.05.038. Epub 2018 May 25.