PMID- 29778202
OWN - NLM
STAT- MEDLINE
DCOM- 20181120
LR  - 20181120
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 219
DP  - 2018 Jun
TI  - Porcine monocyte-derived dendritic cells can be differentially activated by 
      vesicular stomatitis virus and its matrix protein mutants.
PG  - 30-39
LID - S0378-1135(17)31203-8 [pii]
LID - 10.1016/j.vetmic.2018.04.011 [doi]
AB  - Vesicular stomatitis virus (VSV) can cause serious vesicular lesions in pigs, and 
      the matrix (M) protein is its predominant virulence factor. Dendritic cells (DCs) 
      act as the bridge between innate and adaptive immune responses. However, the 
      susceptibility of porcine DCs to VSV infection and the role of M protein in 
      modulating the function of infected DCs are still poorly defined. Thus, this 
      study aimed to determine the ability of virulent wild-type VSV(wtVSV) and two 
      attenuated M protein variants (VSV(DeltaM51) and VSV(MT)) to induce maturation of 
      porcine monocyte-derived DCs (MoDCs) in vitro. It was found that both wtVSV and 
      the M protein mutant VSVs could productively replicate in porcine MoDCs. 
      Infection with wtVSV resulted in weak proinflammatory cytokine responses and 
      interfered with DC maturation via downregulation of the costimulatory molecule 
      complex CD80/86. Whilst VSV(DeltaM51) could activate porcine MoDCs, VSV(MT), a highly 
      attenuated recombinant VSV with triple mutations in the M protein, induced a 
      potent maturation of MoDCs, as evidenced by efficient cytokine induction, and 
      upregulation of CD80/86 and MHC class II. Overall, our findings reveal that 
      porcine MoDCs are differentially activated by VSV, dependent on the presence of a 
      functional M protein. M protein plays a crucial role in modulating porcine DC-VSV 
      interactions. The data further support the potential use of VSV(MT) as a vaccine 
      vector for pigs.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Sun, Guoyan
AU  - Sun G
AD  - School of Agriculture and Biology, Shanghai JiaoTong University, 800 Dongchuan 
      Rd., Shanghai 200240, China.
FAU - Fang, Xinkui
AU  - Fang X
AD  - School of Agriculture and Biology, Shanghai JiaoTong University, 800 Dongchuan 
      Rd., Shanghai 200240, China; Shanghai Municipal Veterinary Key laboratory, 800 
      Dongchuan Rd., Shanghai 200240, China.
FAU - Wu, Hao
AU  - Wu H
AD  - School of Agriculture and Biology, Shanghai JiaoTong University, 800 Dongchuan 
      Rd., Shanghai 200240, China.
FAU - Zhou, Xinchu
AU  - Zhou X
AD  - School of Agriculture and Biology, Shanghai JiaoTong University, 800 Dongchuan 
      Rd., Shanghai 200240, China.
FAU - Ke, Yong
AU  - Ke Y
AD  - School of Agriculture and Biology, Shanghai JiaoTong University, 800 Dongchuan 
      Rd., Shanghai 200240, China.
FAU - Sun, Tao
AU  - Sun T
AD  - School of Agriculture and Biology, Shanghai JiaoTong University, 800 Dongchuan 
      Rd., Shanghai 200240, China; Shanghai Municipal Veterinary Key laboratory, 800 
      Dongchuan Rd., Shanghai 200240, China. Electronic address: tao.sun@sjtu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20180408
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Interleukin-1beta)
RN  - 0 (M protein, Vesicular stomatitis virus)
RN  - 0 (Mutant Proteins)
RN  - 0 (Viral Matrix Proteins)
SB  - IM
MH  - Animals
MH  - Cell Adhesion Molecules/immunology
MH  - Dendritic Cells/drug effects/immunology/*virology
MH  - Interleukin-1beta/biosynthesis/immunology
MH  - Monocytes/immunology/physiology/*virology
MH  - Mutant Proteins/genetics/immunology/pharmacology
MH  - Swine
MH  - Vesicular Stomatitis/virology
MH  - Vesicular stomatitis Indiana virus/drug 
      effects/*genetics/immunology/pathogenicity
MH  - Viral Matrix Proteins/genetics/*pharmacology
OTO - NOTNLM
OT  - Matrix protein
OT  - Monocyte-derived dendritic cells
OT  - Pig
OT  - Vesicular stomatitis virus
EDAT- 2018/05/21 06:00
MHDA- 2018/11/21 06:00
CRDT- 2018/05/21 06:00
PHST- 2017/10/15 00:00 [received]
PHST- 2018/04/03 00:00 [revised]
PHST- 2018/04/06 00:00 [accepted]
PHST- 2018/05/21 06:00 [entrez]
PHST- 2018/05/21 06:00 [pubmed]
PHST- 2018/11/21 06:00 [medline]
AID - S0378-1135(17)31203-8 [pii]
AID - 10.1016/j.vetmic.2018.04.011 [doi]
PST - ppublish
SO  - Vet Microbiol. 2018 Jun;219:30-39. doi: 10.1016/j.vetmic.2018.04.011. Epub 2018 
      Apr 8.