PMID- 29778241
OWN - NLM
STAT- MEDLINE
DCOM- 20190419
LR  - 20190419
IS  - 1872-7484 (Electronic)
IS  - 1566-0702 (Linking)
VI  - 212
DP  - 2018 Jul
TI  - Dual autonomic inhibitory action of central Apelin on gastric motor functions in 
      rats.
PG  - 17-22
LID - S1566-0702(18)30029-8 [pii]
LID - 10.1016/j.autneu.2018.03.005 [doi]
AB  - Centrally administered apelin has been shown to inhibit gastric emptying (GE) in 
      rodents, however, the relevant mechanism has been investigated incompletely. 
      Using male Wistar rats, we investigated the efferent pathways involved in 
      gastroinhibitory action of central apelin. Stereotaxic intracerebroventricular 
      (icv) cannulation, subdiaphragmatic vagotomy (VGX) and/or celiac ganglionectomy 
      (CGX) were performed 7 days prior to the experiments. Apelin-13 was administered 
      (30 nmol, icv) 90 min prior to GE measurement. Nitric oxide synthase inhibitor 
      L-NAME (100 mg/kg), sympatholytic agent guanethidine (5 mg/kg) and/or muscarinic 
      receptor agonist bethanechol (1 mg/kg) were administered intraperitoneally 30 min 
      prior to the central apelin-13 injection. Two strain gages were implanted 
      serosally onto antrum and pylorus to monitor gastric postprandial motility. Heart 
      rate variability (HRV) analysis was performed before and after central vehicle or 
      apelin-13 administration. Apelin-13 delayed solid GE significantly by disturbing 
      coordinated antral and pyloric postprandial contractions. The apelin-induced 
      delayed GE was attenuated partially by CGX or VGX, whereas it was restored 
      completely in rats underwent both CGX and VGX. L-NAME did not change the 
      apelin-induced alterations. Guanethidine or bethanechol restored the 
      apelin-induced gastroinhibition partially, while it was abolished completely in 
      rats received both agents. Apelin-13 decreased the HRV spectral activity in 
      high-frequency range by increasing low-frequency component and the ratio of 
      LF:HF. The present data suggest that (1) both vagal parasympathetic and 
      sympathetic pathways play a role in apelin-induced gastroinhibition, (2) central 
      apelin attenuates vagal cholinergic pathway rather than activating 
      nonadrenergic-noncholinergic pathway. Apelin/APJ receptor system might be 
      candidate for the treatment of autonomic dysfunction and gastrointestinal motor 
      disorders.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Bulbul, Mehmet
AU  - Bulbul M
AD  - Department of Physiology, Akdeniz University, Faculty of Medicine, Antalya, 
      Turkey. Electronic address: mehmetbulbul@akdeniz.edu.tr.
FAU - Sinen, Osman
AU  - Sinen O
AD  - Department of Physiology, Akdeniz University, Faculty of Medicine, Antalya, 
      Turkey.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180328
PL  - Netherlands
TA  - Auton Neurosci
JT  - Autonomic neuroscience : basic & clinical
JID - 100909359
RN  - 0 (Apelin)
SB  - IM
MH  - Animals
MH  - Apelin/administration & dosage/*pharmacology
MH  - Autonomic Nervous System/*drug effects/physiology
MH  - Gastric Emptying/*drug effects/physiology
MH  - Gastrointestinal Motility/*drug effects/physiology
MH  - Gastrointestinal Tract/*drug effects/physiology
MH  - Male
MH  - Postprandial Period/drug effects/physiology
MH  - Rats, Wistar
MH  - Vagus Nerve/drug effects/physiology
OTO - NOTNLM
OT  - Apelin
OT  - Autonomic nervous system
OT  - Gastric emptying
OT  - Gastric motility
EDAT- 2018/05/21 06:00
MHDA- 2019/04/20 06:00
CRDT- 2018/05/21 06:00
PHST- 2018/02/11 00:00 [received]
PHST- 2018/03/15 00:00 [revised]
PHST- 2018/03/27 00:00 [accepted]
PHST- 2018/05/21 06:00 [entrez]
PHST- 2018/05/21 06:00 [pubmed]
PHST- 2019/04/20 06:00 [medline]
AID - S1566-0702(18)30029-8 [pii]
AID - 10.1016/j.autneu.2018.03.005 [doi]
PST - ppublish
SO  - Auton Neurosci. 2018 Jul;212:17-22. doi: 10.1016/j.autneu.2018.03.005. Epub 2018 
      Mar 28.