PMID- 29779272
OWN - NLM
STAT- MEDLINE
DCOM- 20190503
LR  - 20200325
IS  - 2618-0456 (Electronic)
IS  - 1000-1182 (Print)
IS  - 1000-1182 (Linking)
VI  - 36
IP  - 2
DP  - 2018 Apr 1
TI  - [Effects of protein kinase C and motigen-activated protein kinase 
      kinase/extracellular regulated protein kinases signaling pathway on mRNA level of 
      inducible nitric oxide synthase in Tca8113 cells].
PG  - 133-139
LID - 10.7518/hxkq.2018.02.004 [doi]
AB  - OBJECTIVE: To explore the regulatory mechanism of inducible nitric oxide synthase 
      (NOS-2) expression related to proliferation of Tca8113 cells. METHODS: RNAi 
      mediated by short hairpin RNAs was utilized to knock down NOS-2, protein kinase C 
      (PKC)-alpha, PKC-beta and PKC-delta. Griess Reagent played a significant role on the 
      detection of NO product after NOS-2 silence. The cell proliferation was 
      determined by CCK8 method. Quantitative real-time polymerase chain reaction 
      (q-PCR) was recruited to check the mRNA level of NOS-2, PKC-alpha, PKC-beta and PKC-delta 
      after treated by a variety of ways. Eventually, the measure of phosphorylation of 
      extracellular regulated protein kinases (ERK)1/2 was performed by Western 
      blotting in PMA-treated Tca8113 cells. RESULTS: The cell viability of Tca8113 
      decreased obviously after transfected with NOS-2 siRNA (P<0.01). PKC reduced the 
      expression level of NOS-2 mRNA (P<0.05). PKC-alpha, PKC-beta and PKC-delta worked together 
      to regulate the level of NOS-2 mRNA (P<0.01). Motigen-activated protein kinase 
      kinase (MEK)/ERK signaling pathway regulated the level of NOS-2 mRNA negatively 
      (P<0.05). PKC down regulated the level of NOS-2 mRNA through MEK/ERK signaling 
      pathway (P<0.05). CONCLUSIONS: PKC regulates the mRNA level of NOS-2 related to 
      proliferation through MEK/ERK signaling pathway in Tca8113 cells.
FAU - Gao, Xue-Feng
AU  - Gao XF
AD  - The Affiliated Stomatological Hospital of Harbin Medical University, Harbin 
      150001, China.
FAU - Jiao, Hai-Bin
AU  - Jiao HB
AD  - The Affiliated Stomatological Hospital of Harbin Medical University, Harbin 
      150001, China.
FAU - Ye, Chang-Cheng
AU  - Ye CC
AD  - The Affiliated Stomatological Hospital of Harbin Medical University, Harbin 
      150001, China.
FAU - Liu, Ying-Qun
AU  - Liu YQ
AD  - The Affiliated Stomatological Hospital of Harbin Medical University, Harbin 
      150001, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Hua Xi Kou Qiang Yi Xue Za Zhi
JT  - Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal 
      of stomatology
JID - 9422648
RN  - 0 (RNA, Messenger)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Cell Proliferation
MH  - Extracellular Signal-Regulated MAP Kinases
MH  - Gene Knockdown Techniques
MH  - Nitric Oxide
MH  - Nitric Oxide Synthase
MH  - Nitric Oxide Synthase Type II/metabolism
MH  - *Protein Kinase C/metabolism/physiology
MH  - *RNA, Messenger/metabolism
MH  - *Signal Transduction
PMC - PMC7030351
OTO - NOTNLM
OT  - cell proliferation
OT  - inducible nitric oxide synthase
OT  - motigen-activated protein kinase kinase/extracellular regulated protein kinases 
      signaling pathway
OT  - protein kinase C
EDAT- 2018/05/21 06:00
MHDA- 2019/05/06 06:00
PMCR- 2018/04/01
CRDT- 2018/05/21 06:00
PHST- 2018/05/21 06:00 [entrez]
PHST- 2018/05/21 06:00 [pubmed]
PHST- 2019/05/06 06:00 [medline]
PHST- 2018/04/01 00:00 [pmc-release]
AID - wcjs-36-02-133 [pii]
AID - 10.7518/hxkq.2018.02.004 [doi]
PST - ppublish
SO  - Hua Xi Kou Qiang Yi Xue Za Zhi. 2018 Apr 1;36(2):133-139. doi: 
      10.7518/hxkq.2018.02.004.