PMID- 29780216
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240314
IS  - 1054-5476 (Print)
IS  - 1054-5476 (Linking)
VI  - 54
IP  - 3
DP  - 2018
TI  - Rapid genotype "independent" Zea mays L. (maize) transformation via direct 
      somatic embryogenesis.
PG  - 240-252
LID - 10.1007/s11627-018-9905-2 [doi]
AB  - Constitutive expression of the Zea mays L. (maize) morphogenic transcription 
      factors Baby Boom (Bbm) and Wuschel2 (Wus2) in maize can not only greatly 
      increase transformation efficiency but can also induce phenotypic abnormalities 
      and sterility. In an effort to alleviate the pleiotropic effects of constitutive 
      expression, a genome wide search was undertaken to find suitable maize promoters 
      to drive tissue and timing-specific expression of the transformation enhancing 
      genes Bbm and Wus2. A promoter from a maize phospholipid transferase protein gene 
      (Zm-PLTP(pro) ) was identified based on its expression in leaves, embryos, and 
      callus while being downregulated in roots, meristems, and reproductive tissues. 
      When Zm-PLTP(pro) driving Bbm was transformed into immature maize embryos along 
      with a Wus2 expression cassette driven by the nopaline synthase promoter 
      (Nos(pro) ::Wus2) abundant somatic embryos rapidly formed on the scutella. These 
      embryos were individual and uniformly transformed and could be directly 
      germinated into plants without a callus phase. Transformed plants could be sent 
      to the greenhouse in as little as 1 mo and regenerated plants matched the 
      seed-derived phenotype for the inbred and were fertile. However, T1 seed from 
      these plants had poor germination. Replacing Nos(pro) with a maize 
      auxin-inducible promoter (Zm-Axig1(pro) ) in combination with Zm-PLTP(pro) ::Bbm, 
      allowed healthy, fertile plants to be regenerated. Single-copy T1 seed germinated 
      normally and had a predominantly wild-type inbred phenotype. For maize, this 
      callus-free transformation process has worked in all inbred lines tested.
FAU - Lowe, Keith
AU  - Lowe K
AD  - DuPont Pioneer, Johnston, IA USA. ISNI: 0000 0004 0414 655X. GRID: grid.292487.2
FAU - La Rota, Mauricio
AU  - La Rota M
AD  - DuPont Pioneer, Johnston, IA USA. ISNI: 0000 0004 0414 655X. GRID: grid.292487.2
FAU - Hoerster, George
AU  - Hoerster G
AD  - DuPont Pioneer, Johnston, IA USA. ISNI: 0000 0004 0414 655X. GRID: grid.292487.2
FAU - Hastings, Craig
AU  - Hastings C
AD  - DuPont Pioneer, Johnston, IA USA. ISNI: 0000 0004 0414 655X. GRID: grid.292487.2
FAU - Wang, Ning
AU  - Wang N
AD  - DuPont Pioneer, Johnston, IA USA. ISNI: 0000 0004 0414 655X. GRID: grid.292487.2
FAU - Chamberlin, Mark
AU  - Chamberlin M
AD  - DuPont Pioneer, Johnston, IA USA. ISNI: 0000 0004 0414 655X. GRID: grid.292487.2
FAU - Wu, Emily
AU  - Wu E
AD  - DuPont Pioneer, Johnston, IA USA. ISNI: 0000 0004 0414 655X. GRID: grid.292487.2
FAU - Jones, Todd
AU  - Jones T
AD  - DuPont Pioneer, Johnston, IA USA. ISNI: 0000 0004 0414 655X. GRID: grid.292487.2
FAU - Gordon-Kamm, William
AU  - Gordon-Kamm W
AUID- ORCID: 0000-0002-4852-751X
AD  - DuPont Pioneer, Johnston, IA USA. ISNI: 0000 0004 0414 655X. GRID: grid.292487.2
LA  - eng
PT  - Journal Article
DEP - 20180430
PL  - United States
TA  - In Vitro Cell Dev Biol Plant
JT  - In vitro cellular & developmental biology. Plant : journal of the Tissue Culture 
      Association
JID - 9112624
PMC - PMC5954046
OTO - NOTNLM
OT  - BabyBoom
OT  - Direct embryogenesis
OT  - Maize transformation
OT  - Wuschel
EDAT- 2018/05/22 06:00
MHDA- 2018/05/22 06:01
PMCR- 2018/04/30
CRDT- 2018/05/22 06:00
PHST- 2018/01/10 00:00 [received]
PHST- 2018/03/26 00:00 [accepted]
PHST- 2018/05/22 06:00 [entrez]
PHST- 2018/05/22 06:00 [pubmed]
PHST- 2018/05/22 06:01 [medline]
PHST- 2018/04/30 00:00 [pmc-release]
AID - 9905 [pii]
AID - 10.1007/s11627-018-9905-2 [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol Plant. 2018;54(3):240-252. doi: 10.1007/s11627-018-9905-2. 
      Epub 2018 Apr 30.