PMID- 29780629
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220317
IS  - 2072-1439 (Print)
IS  - 2077-6624 (Electronic)
IS  - 2072-1439 (Linking)
VI  - 10
IP  - Suppl 7
DP  - 2018 Apr
TI  - Performance evaluation of detecting circulating tumor cells and tumor cells in 
      bronchoalveolar lavage fluid in diagnosis of peripheral lung cancer.
PG  - S830-S837
LID - 10.21037/jtd.2017.12.125 [doi]
AB  - BACKGROUND: To evaluate the diagnostic performances of detecting circulating 
      tumor cells (CTCs) and tumor cells in bronchoalveolar lavage fluid (BALF) for 
      peripheral lung cancer. METHODS: A total of 247 patients with lung cancer and 70 
      cases with benign lung disease were recruited in this study. Peripheral blood and 
      BALF samples were collected, in which the tumor cells were enriched by negative 
      immunomagnetic selection and detected by fluorescence in situ hybridization 
      (FISH) of chromosome enumeration probe 8 (CEP8). The levels of tumor-associated 
      markers (e.g., CEA, CA125, and NSE) in peripheral blood plasma were measured by 
      using electrochemiluminescence. RESULTS: The numbers of CTCs detected in 
      peripheral blood were significantly higher in patients with lung cancer than 
      those with benign lung disease (5.78+/-0.57 vs. 1.13+/-0.39, Z=-8.64, P<0.01). 
      Similarly, tumor cells count in BALF of malignancy were higher than that of 
      benign lesions (6.76+/-0.89 vs. 0.89+/-0.23, Z=-6.254, P<0.01). However, as for 
      patients with lung cancer and benign lung disease, the numbers of tumor cells in 
      peripheral blood were comparable with those in BALF (both P>0.05). Detecting CTCs 
      and tumor cells in BALF had similar areas under curves (AUC =0.871 and 0.963, 
      respectively; P>0.05) in discriminating benign lesions from lung cancer 
      (sensitivity 83.8% and 92.6%, specificity 86.5% and 99.9%, respectively), both of 
      which were larger than those of NSE, CEA, and CA125 (AUC =0.564, 0.512 and 0.554, 
      respectively; all P<0.05). The diagnostic performances of discriminating benign 
      lesions and lung cancer in BALF and peripheral blood were both in concordance 
      with that of histopathology (kappa values 0.662 and 0.569, respectively; both 
      P<0.001). CONCLUSIONS: Detecting tumor cells in peripheral blood and BALF may 
      sensitive to identify benign and malignant peripheral lung lesions and be of 
      value for early diagnosis of lung cancer.
FAU - Zhong, Chang-Hao
AU  - Zhong CH
AD  - State Key Laboratory of Respiratory Disease, National Clinical Research Center 
      for Respiratory Disease, Guangzhou Institute of Respiratory Health, First 
      Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China.
FAU - Tong, Da
AU  - Tong D
AD  - Department of Respiratory Medicine, Fifth Affiliated Hospital of Guangzhou 
      Medical University, Guangzhou 510700, China.
FAU - Zhou, Zi-Qing
AU  - Zhou ZQ
AD  - State Key Laboratory of Respiratory Disease, National Clinical Research Center 
      for Respiratory Disease, Guangzhou Institute of Respiratory Health, First 
      Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China.
FAU - Su, Zhu-Quan
AU  - Su ZQ
AD  - State Key Laboratory of Respiratory Disease, National Clinical Research Center 
      for Respiratory Disease, Guangzhou Institute of Respiratory Health, First 
      Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China.
FAU - Luo, Yu-Long
AU  - Luo YL
AD  - State Key Laboratory of Respiratory Disease, National Clinical Research Center 
      for Respiratory Disease, Guangzhou Institute of Respiratory Health, First 
      Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China.
FAU - Xing, Jia
AU  - Xing J
AD  - Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union 
      Medical College, Kunming 650118, China.
FAU - Bai, Ya-Li
AU  - Bai YL
AD  - Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union 
      Medical College, Kunming 650118, China.
FAU - Guo, Su-Jie
AU  - Guo SJ
AD  - Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union 
      Medical College, Kunming 650118, China.
FAU - Li, Shi-Yue
AU  - Li SY
AD  - State Key Laboratory of Respiratory Disease, National Clinical Research Center 
      for Respiratory Disease, Guangzhou Institute of Respiratory Health, First 
      Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - J Thorac Dis
JT  - Journal of thoracic disease
JID - 101533916
PMC - PMC5945685
OTO - NOTNLM
OT  - Lung cancer
OT  - bronchoalveolar lavage
OT  - circulating tumor cell (CTC)
OT  - diagnosis
COIS- Conflicts of Interest: The authors have no conflicts of interest to declare.
EDAT- 2018/05/22 06:00
MHDA- 2018/05/22 06:01
PMCR- 2018/04/01
CRDT- 2018/05/22 06:00
PHST- 2018/05/22 06:00 [entrez]
PHST- 2018/05/22 06:00 [pubmed]
PHST- 2018/05/22 06:01 [medline]
PHST- 2018/04/01 00:00 [pmc-release]
AID - jtd-10-S7-S830 [pii]
AID - 10.21037/jtd.2017.12.125 [doi]
PST - ppublish
SO  - J Thorac Dis. 2018 Apr;10(Suppl 7):S830-S837. doi: 10.21037/jtd.2017.12.125.