PMID- 29782033
OWN - NLM
STAT- MEDLINE
DCOM- 20190410
LR  - 20190410
IS  - 1873-3468 (Electronic)
IS  - 0014-5793 (Linking)
VI  - 592
IP  - 11
DP  - 2018 Jun
TI  - The membrane-bound O-acyltransferase Ale1 transfers an acyl moiety to newly 
      synthesized 2-alkyl-sn-glycero-3-phosphocholine in yeast.
PG  - 1829-1836
LID - 10.1002/1873-3468.13103 [doi]
AB  - To elucidate the mechanism of acyl chain remodeling at the sn-1 position of 
      phosphatidylcholine (PC), we investigated acyl chain introduction using a newly 
      synthesized 1-hydroxy-2-hexadecyl-sn-glycero-3-phosphocholine (HHPC) in 
      Saccharomyces cerevisiae. HHPC is incorporated into yeast cells and converted to 
      a PC species containing acyl residues of 16 or 18 carbons. The efficiency of 
      palmitoleic acid introduction to HHPCin vitro is lower in the reaction with the 
      extract from the deletion mutant of ALE1, which encodes a membrane-bound 
      O-acyltransferase, than in that with extracts from the wild-type strain. In 
      addition, deletion of ALE1 causes reductions in the molecular species containing 
      acyl residues in HHPC. These results reveal that ALE1 is involved in acyl chain 
      transfer to the sn-1 position of HHPC in yeast.
CI  - (c) 2018 Federation of European Biochemical Societies.
FAU - Morisada, Shiho
AU  - Morisada S
AD  - Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Japan.
FAU - Ono, Yusuke
AU  - Ono Y
AD  - Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Japan.
FAU - Kodaira, Teruhisa
AU  - Kodaira T
AD  - Department of Applied Biological Chemistry, The University of Tokyo, Bunkyo-ku, 
      Japan.
FAU - Kishino, Hideyuki
AU  - Kishino H
AD  - Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Japan.
FAU - Ninomiya, Ryo
AU  - Ninomiya R
AD  - Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Japan.
FAU - Mori, Naoki
AU  - Mori N
AD  - Department of Applied Biological Chemistry, The University of Tokyo, Bunkyo-ku, 
      Japan.
FAU - Watanabe, Hidenori
AU  - Watanabe H
AD  - Department of Applied Biological Chemistry, The University of Tokyo, Bunkyo-ku, 
      Japan.
FAU - Ohta, Akinori
AU  - Ohta A
AD  - Department of Biological Chemistry, College of Bioscience and Biotechnology, 
      Chubu University, Kasugai, Japan.
FAU - Horiuchi, Hiroyuki
AU  - Horiuchi H
AD  - Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Japan.
FAU - Fukuda, Ryouichi
AU  - Fukuda R
AD  - Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Japan.
LA  - eng
PT  - Letter
PT  - Research Support, Non-U.S. Gov't
DEP - 20180528
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 2.3.1.23 (1-Acylglycerophosphocholine O-Acyltransferase)
RN  - EC 2.3.1.23 (ALE1 protein, S cerevisiae)
SB  - IM
MH  - 1-Acylglycerophosphocholine O-Acyltransferase/genetics/*metabolism
MH  - Gene Deletion
MH  - Phosphatidylcholines/*biosynthesis/genetics
MH  - Saccharomyces cerevisiae/*enzymology/genetics
MH  - Saccharomyces cerevisiae Proteins/genetics/*metabolism
OTO - NOTNLM
OT  - Saccharomyces cerevisiae
OT  - acyl chain
OT  - acyltransferase
OT  - phosphatidylcholine
OT  - remodeling
OT  - sn-1 position
EDAT- 2018/05/22 06:00
MHDA- 2019/04/11 06:00
CRDT- 2018/05/22 06:00
PHST- 2018/04/02 00:00 [received]
PHST- 2018/05/10 00:00 [revised]
PHST- 2018/05/11 00:00 [accepted]
PHST- 2018/05/22 06:00 [pubmed]
PHST- 2019/04/11 06:00 [medline]
PHST- 2018/05/22 06:00 [entrez]
AID - 10.1002/1873-3468.13103 [doi]
PST - ppublish
SO  - FEBS Lett. 2018 Jun;592(11):1829-1836. doi: 10.1002/1873-3468.13103. Epub 2018 
      May 28.