PMID- 29783731
OWN - NLM
STAT- MEDLINE
DCOM- 20181009
LR  - 20181114
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 10
IP  - 5
DP  - 2018 May 19
TI  - Lactobacillus plantarum Strain Ln4 Attenuates Diet-Induced Obesity, Insulin 
      Resistance, and Changes in Hepatic mRNA Levels Associated with Glucose and Lipid 
      Metabolism.
LID - 10.3390/nu10050643 [doi]
LID - 643
AB  - The prevalence of obesity and associated metabolic disorders, including diabetes 
      and cardiovascular disease, is rapidly becoming a severe global health problem. 
      Recent reports have suggested that the alteration of the gut ecosystem through 
      the consumption of probiotics and fermented foods, such as yogurt and Kimchi, can 
      significantly impact obesity and Type 2 diabetes (T2D)-related biomarkers. In 
      this study, we screened over 400 strains of lactic acid bacteria (LAB) that were 
      isolated from fermented foods to identify potent anti-obesogenic and diabetic 
      probiotics in vitro. Of the strains tested, Lactobacillus plantarum Ln4 (Ln4), 
      which was obtained from napa cabbage kimchi, significantly reduced lipid 
      accumulation and stimulated glucose uptake in 3T3-L1 adipocytes. Oral 
      administration of Ln4 reduced weight gain and epididymal fat mass in mice fed on 
      a high-fat diet (HFD). Total plasma triglyceride level was significantly lower in 
      mice that were treated Ln4 as compared with mice fed HFD. The protein levels of 
      adipokines such as C-reactive protein (CRP), insulin-like growth factor binding 
      proteins-3 (IGFBP-3), and monocyte chemoattractant protein-1 (MCP-1) decreased in 
      white adipose tissues of Ln4-treated mice. Furthermore, these mice exhibited a 
      significant reduction of insulin resistance index (HOMA-IR) and the improvement 
      of glucose tolerance (OGTT) and insulin response (ITT) following Ln4 
      administration. This was associated with changes in several hepatic gene 
      expressions (increased mRNA levels of IRS2, Akt2, AMPK, LPL, and reduced CD36) 
      that regulate glucose and lipid metabolism. Taken together, these results 
      indicate that in vitro and in vivo Ln4 treatment attenuates diet-induced obesity 
      and T2D biomarkers, highlighting the potential of Ln4 as a therapeutic probiotic 
      agent for metabolic disorders.
FAU - Lee, Eunjung
AU  - Lee E
AD  - Research group of Traditional Food, Korea Food Research Institute, Wanju 55365, 
      Korea. ejlee@kfri.re.kr.
FAU - Jung, So-Ra
AU  - Jung SR
AD  - Research group of Traditional Food, Korea Food Research Institute, Wanju 55365, 
      Korea. jarasasa2@gmail.com.
FAU - Lee, So-Young
AU  - Lee SY
AD  - Research Group of Natural Materials and Metabolism, Korea Food Research 
      Institute, Wanju 55365, Korea. sylee09@kfri.re.kr.
FAU - Lee, Na-Kyoung
AU  - Lee NK
AD  - Department of Food Science and Biotechnology of Animal Resources, Konkuk 
      University, Seoul 05029, Korea. lnk11@konkuk.ac.kr.
FAU - Paik, Hyun-Dong
AU  - Paik HD
AD  - Department of Food Science and Biotechnology of Animal Resources, Konkuk 
      University, Seoul 05029, Korea. hdpaik@konkuk.ac.kr.
FAU - Lim, Seong-Il
AU  - Lim SI
AD  - Research Group of Healthcare, Korea Food Research Institute, Wanju 55365, Korea. 
      silim@kfri.re.kr.
LA  - eng
PT  - Journal Article
DEP - 20180519
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (Adipokines)
RN  - 0 (Biomarkers)
RN  - 0 (Blood Glucose)
RN  - 0 (Lipids)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/metabolism/microbiology
MH  - Adipogenesis
MH  - Adipokines/blood
MH  - Animals
MH  - Biomarkers/blood
MH  - Blood Glucose/*metabolism
MH  - *Diet, High-Fat
MH  - Disease Models, Animal
MH  - *Gastrointestinal Microbiome
MH  - Gene Expression Regulation
MH  - *Insulin Resistance
MH  - Lactobacillus plantarum/*physiology
MH  - Lipids/*blood
MH  - Liver/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Obesity/blood/etiology/microbiology/*prevention & control
MH  - Probiotics/*administration & dosage
MH  - RNA, Messenger/genetics/*metabolism
MH  - Time Factors
MH  - Weight Gain
PMC - PMC5986522
OTO - NOTNLM
OT  - high fat diet
OT  - insulin resistance
OT  - insulin signaling
OT  - lactobacillus plantarum Ln4
OT  - metabolic disorder
OT  - obesity
COIS- All authors have declared that no conflict of interest exists.
EDAT- 2018/05/23 06:00
MHDA- 2018/10/10 06:00
PMCR- 2018/05/01
CRDT- 2018/05/23 06:00
PHST- 2018/03/16 00:00 [received]
PHST- 2018/05/09 00:00 [revised]
PHST- 2018/05/17 00:00 [accepted]
PHST- 2018/05/23 06:00 [entrez]
PHST- 2018/05/23 06:00 [pubmed]
PHST- 2018/10/10 06:00 [medline]
PHST- 2018/05/01 00:00 [pmc-release]
AID - nu10050643 [pii]
AID - nutrients-10-00643 [pii]
AID - 10.3390/nu10050643 [doi]
PST - epublish
SO  - Nutrients. 2018 May 19;10(5):643. doi: 10.3390/nu10050643.