PMID- 29783968
OWN - NLM
STAT- MEDLINE
DCOM- 20181002
LR  - 20181114
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 14
IP  - 1
DP  - 2018 May 21
TI  - Investigating porcine parvoviruses genogroup 2 infection using in situ polymerase 
      chain reaction.
PG  - 163
LID - 10.1186/s12917-018-1487-z [doi]
LID - 163
AB  - BACKGROUND: Porcine parvovirus 2 (PPV2) was detected in swine serum without 
      showing any relationship with disease. The emergence of the virus seemed to be a 
      unique event until other genetically highly similar parvoviruses were identified 
      in China and, later in 2012, the presence of the virus was also described in 
      Europe. PPV2 is widely distributed in pig populations where it is suspected to be 
      involved in respiratory conditions, based on its frequent detection in lung 
      samples. In order to investigate the potential pathogenic involvement of PPV2, 60 
      dead pigs were examined from two farms. They were necropsied and tested for PPV2 
      and PCV2 (Porcine circovirus type 2) by PCR; by Brown and Brenn (B&B) staining 
      for bacteria; by immunohistochemistry (IHC) to detect CD3, Swine leukocyte 
      antigen class II DQ (SLAIIDQ), lysozyme, porcine reproductive and respiratory 
      syndrome virus (PRRSV), swine influenza (SIV), Mycoplasma hyopneumoniae (Mhyo); 
      and by in situ hybridization (ISH) to detect ssDNA and dsDNA of PCV2. PPV2 
      positive samples were subjected to in situ polymerase chain reaction (IS-PCR) 
      including double staining method to detect PPV2 and host cell markers. To 
      calculate statistical difference we used GENMOD or LOGISTIC procedures in 
      Statistical Analysis System (SAS(R)). RESULTS: We found that the PPV2 was localized 
      mostly in lymphocytes in lungs, lymph nodes and liver. Neither CD3 antigen nor 
      lysozyme was expressed by these infected cells. In contrast, low levels of 
      SLAIIDQ were expressed by infected cells, suggesting that PPV2 may have a 
      specific tropism for immature B lymphocytes and/or NK lymphocytes though possibly 
      not T lymphocytes. CONCLUSION: The overall conclusion of this study indicates 
      that PPV2 may contribute to the pathogenesis of pneumonia.
FAU - Novosel, Dinko
AU  - Novosel D
AUID- ORCID: 0000-0003-2602-8696
AD  - Department of Pathology, Croatian Veterinary Institute, Savska cesta 143, 10000, 
      Zagreb, Croatia. dinko.novosel@gmail.com.
AD  - Department for Animal science, Faculty of Agriculture, University of Zagreb, 
      Svetosimunska 25, 10000, Zagreb, Croatia. dinko.novosel@gmail.com.
FAU - Cadar, Daniel
AU  - Cadar D
AD  - WHO Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and 
      Research, National Reference Centre for Tropical Infectious Diseases, Bernhard 
      Nocht Institute for Tropical Medicine, Bernhard-Nocht-Strasse 74, 20359, Hamburg, 
      Germany.
AD  - Department of Microbiology and Infectious Diseases, Faculty of Veterinary 
      Science, Immunology, Szent Istvan University, Istvan u. 2, Budapest, 1078, 
      Hungary.
FAU - Tuboly, Tamas
AU  - Tuboly T
AD  - Department of Microbiology and Infectious Diseases, Faculty of Veterinary 
      Science, Immunology, Szent Istvan University, Istvan u. 2, Budapest, 1078, 
      Hungary.
FAU - Jungic, Andreja
AU  - Jungic A
AD  - Department for Virology, Croatian Veterinary Institute, Savska cesta 143, 10000, 
      Zagreb, Croatia.
FAU - Stadejek, Tomasz
AU  - Stadejek T
AD  - Department of Pathology and Veterinary Diagnostic, Faculty of Veterinary 
      Medicine, University of Life Science, Nowoursynowska 159C, 02-776, Warsaw, 
      Poland.
FAU - Ait-Ali, Tahar
AU  - Ait-Ali T
AD  - The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of 
      Edinburgh, Easter Bush, Edingburgh, United Kingdom.
FAU - Csagola, Attila
AU  - Csagola A
AD  - Department of Microbiology and Infectious Diseases, Faculty of Veterinary 
      Science, Immunology, Szent Istvan University, Istvan u. 2, Budapest, 1078, 
      Hungary.
LA  - eng
GR  - BB/J004227/1/BBSRC Institute Strategic Programme grants/
GR  - BB/P013740/1/BBSRC Institute Strategic Programme grants/
PT  - Journal Article
DEP - 20180521
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
SB  - IM
MH  - Animals
MH  - Parvoviridae Infections/diagnosis/*veterinary/virology
MH  - Parvovirus, Porcine/*genetics
MH  - Real-Time Polymerase Chain Reaction/methods/*veterinary
MH  - Swine
MH  - Swine Diseases/*diagnosis/virology
PMC - PMC5963090
OTO - NOTNLM
OT  - In situ PCR
OT  - Lungs
OT  - Lymphocytes
OT  - PPV2
COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: Authors had obtained a written 
      consent from farm owners to collect samples. COMPETING INTERESTS: The authors 
      declare that they have no competing interests. PUBLISHER'S NOTE: Springer Nature 
      remains neutral with regard to jurisdictional claims in published maps and 
      institutional affiliations.
EDAT- 2018/05/23 06:00
MHDA- 2018/10/03 06:00
PMCR- 2018/05/21
CRDT- 2018/05/23 06:00
PHST- 2017/10/10 00:00 [received]
PHST- 2018/05/07 00:00 [accepted]
PHST- 2018/05/23 06:00 [entrez]
PHST- 2018/05/23 06:00 [pubmed]
PHST- 2018/10/03 06:00 [medline]
PHST- 2018/05/21 00:00 [pmc-release]
AID - 10.1186/s12917-018-1487-z [pii]
AID - 1487 [pii]
AID - 10.1186/s12917-018-1487-z [doi]
PST - epublish
SO  - BMC Vet Res. 2018 May 21;14(1):163. doi: 10.1186/s12917-018-1487-z.