PMID- 29784578
OWN - NLM
STAT- MEDLINE
DCOM- 20181217
LR  - 20181217
IS  - 1876-035X (Electronic)
IS  - 1876-0341 (Linking)
VI  - 11
IP  - 6
DP  - 2018 Nov-Dec
TI  - Quantitative study about the role of environmental conditions in the survival 
      capability of multidrug-resistant bacteria.
PG  - 801-806
LID - S1876-0341(18)30069-8 [pii]
LID - 10.1016/j.jiph.2018.05.001 [doi]
AB  - BACKGROUND: Healthcare-associated infections (HAIs) caused by multidrug-resistant 
      bacteria (MDRB) are of global concern and hospital textiles can contribute to 
      their transmission. MDRB are able to survive on textiles for more than enough 
      time to spread in the environment. Some studies summarized the effect of 
      environmental factors on the duration of bacterial survival, but it remained an 
      open question how these factors influence the quantity of surviving bacteria in a 
      period of a few days, which is relevant from the perspective of HAIs. 
      Investigating this effect can contribute to better understand the spread of MDRB 
      and the emergence of hospital outbreaks. METHODS: We investigated quantitatively 
      the survival capability of 15 vancomycin-resistant Enterococcus faecium (VRE), 15 
      methicillin-resistant Staphylococcus aureus (MRSA), 15 multidrug-resistant 
      Acinetobacter baumannii (MACI) and 15 multidrug-resistant Klebsiella pneumoniae 
      (MRKP) in five environmental conditions using the plate count method. We examined 
      the role of nutrients, textile types, temperature and level of relative humidity 
      on bacterial survival after 1-7days of incubation. RESULTS: Each bacterial group 
      showed higher survival capability on 100% cotton towel than on 100% cotton sheet 
      (P<0.01). MRSAs and VREs showed higher (P<0.01), MACIs showed lower (P=0.02) 
      CFU/swatch values on 100% polyester sheet than on cotton sheet. The survival 
      capability of MRKPs and MRSAs was higher inoculated in nutrient broth than in 
      saline solution (P<0.01). Each bacterial group showed lower survival capability 
      (P<0.01) at body condition (T=35 degrees C, Rh=83%) than at control (T=25 degrees C, Rh=52%). 
      CONCLUSIONS: Towels proved to be excellent conditions for each bacteria to 
      survive, however chemical composition of the textiles affected differently the 
      survival of Gram-positive and Gram-negative bacteria. These findings could be 
      useful in searching for the source of outbreaks. Organic contamination of the 
      textiles can increase the survival of desiccation-sensitive bacteria, therefore 
      nutrient-rich inoculating medium is recommended in survival studies.
CI  - Copyright (c) 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Hanczvikkel, Adrienn
AU  - Hanczvikkel A
AD  - Doctoral School on Material Sciences and Technologies, Obuda University, Becsi 
      str. 96/b, Budapest H-1034, Hungary. Electronic address: 
      hanczvikkel.adrienn@tmpk.uni-obuda.hu.
FAU - Toth, Akos
AU  - Toth A
AD  - Department of Bacteriology, National Public Health Institute, Budapest, Hungary.
LA  - eng
PT  - Journal Article
DEP - 20180518
PL  - England
TA  - J Infect Public Health
JT  - Journal of infection and public health
JID - 101487384
SB  - IM
MH  - Acinetobacter baumannii/drug effects/*physiology/radiation effects
MH  - Colony Count, Microbial
MH  - Drug Resistance, Multiple, Bacterial
MH  - Enterococcus faecium/drug effects/*physiology/radiation effects
MH  - *Environmental Exposure
MH  - *Environmental Microbiology
MH  - Humidity
MH  - Klebsiella pneumoniae/drug effects/*physiology/radiation effects
MH  - *Microbial Viability
MH  - Pilot Projects
MH  - Staphylococcus aureus/drug effects/*physiology/radiation effects
MH  - Temperature
MH  - Textiles
OTO - NOTNLM
OT  - Environmental factors
OT  - Healthcare-associated bacteria
OT  - Survival capability
OT  - Textiles
EDAT- 2018/05/23 06:00
MHDA- 2018/12/18 06:00
CRDT- 2018/05/23 06:00
PHST- 2018/01/18 00:00 [received]
PHST- 2018/04/27 00:00 [revised]
PHST- 2018/05/03 00:00 [accepted]
PHST- 2018/05/23 06:00 [pubmed]
PHST- 2018/12/18 06:00 [medline]
PHST- 2018/05/23 06:00 [entrez]
AID - S1876-0341(18)30069-8 [pii]
AID - 10.1016/j.jiph.2018.05.001 [doi]
PST - ppublish
SO  - J Infect Public Health. 2018 Nov-Dec;11(6):801-806. doi: 
      10.1016/j.jiph.2018.05.001. Epub 2018 May 18.