PMID- 29786204
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 30
IP  - 10
DP  - 2016 Oct 8
TI  - [STUDY ON MOLECULAR MECHANISM OF OSTEOCLAST DIFFERENTIATION INDUCED BY 
      STAPHYLOCOCCAL PEPTIDOGLYCAN].
PG  - 1244-1248
LID - 10.7507/1002-1892.20160254 [doi]
AB  - OBJECTIVE: To investigate the molecular mechanism of osteoclast differentiation 
      induced by Staphylococcal peptidoglycan (PGN-sa). METHODS: Raw264.7 cells were 
      stimulated with PGN-sa and with PGN-sa+SC75741[a potent inhibitor of nuclear 
      factor kappaB (NF-kappaB) activation] in a concentration of 200 ng/mL. The protein 
      expression of nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) was 
      tested at 0, 1, 2, and 3 days; the proteins related to osteoclast differentiation 
      of extracellular regulated protein kinases (ERK), p38, c-Jun N-terminal kinase 
      (JNK), NF-kappaB, inhibitor of NF-kappaB (IkappaB-alpha), Akt, and the phosphorylation forms of 
      p38, ERK, JNK, Akt, NF-kappaB were measured at 0, 5, 10, 20, 40, and 60 minutes by 
      Western blot. In addition, Raw264.7 cells were stimulated with PGN-sa in the 
      concentrations of 100 ng/mL (group A), 200 ng/mL (group B), 400 ng/mL (group C), 
      and with PBS (group D) for 1, 2, and 3 days; the expression levels of tumor 
      necrosis factor alpha (TNF-alpha), interleukin 1alpha (IL-1alpha), and IL-6 were detected by 
      ELISA. RESULTS: The results of Western blot showed that the expression of NFATc1 
      increased gradually with time, showing significant difference between different 
      time points (P<0.05). However, after SC75741 was added, the expression of NFATc1 
      was inhibited at 2 and 3 days, showing significant difference when compared with 
      no addition of SC75741 (P<0.001). After stimulation of PGN-sa, the expression of 
      IkB-alpha decreased significantly at 5 and 10 minutes when compared with those at the 
      other time points (P<0.001), and returned to normal at 20 minutes. Meanwhile, the 
      expression of p-NF-kappaB increased significantly at 5 and 10 minutes when compared 
      with those at the other time points (P<0.001), and returned to normal at 20 
      minutes; and the expression of p-NF-kappaB at 5 minutes was significantly higher than 
      that at 10 minutes (P<0.001). After the addition of SC75741, there was no change 
      in the expressions of IkappaB-alpha and p-NF-kappaB, showing no significant difference 
      between different time points P>0.05). Moreover, the expressions of ERK, p38, 
      JNK, NF-kappaB, Akt, p-p38, p-ERK, p-JNK, and p-Akt showed no significant change 
      between different time points P>0.05). ELISA results showed that there were no 
      expressions of TNF-alpha and IL-1alpha in groups A-D at different time points. The 
      expression of IL-6 had an increasing trend with time prolonged in each group, 
      showing significant differences between different time points (P<0.05). Moreover, 
      at 1 day after culture, the expression of IL-6 showed no significant difference 
      among groups P>0.05). At 2 and 3 days after culture, the expression of IL-6 in 
      groups A-C showed an increasing trend and was significantly higher than that in 
      group D, showing significant difference among groups (P<0.05). CONCLUSIONS: 
      PGN-sa can promote osteoclast differentiation through NF-kappaB signaling pathway, 
      and IL-6 may play a role in this process.
FAU - Ren, Lirong
AU  - Ren L
AD  - Kunming Medical University, Kunming Yunnan, 650504, P. R. China.
FAU - Xu, Yongqing
AU  - Xu Y
AD  - Department of Orthopedics, Kunming General Hospital of Chengdu Military Region.
FAU - Wang, Hai
AU  - Wang H
AD  - Department of Orthopedics, Kunming General Hospital of Chengdu Military Region.
FAU - He, Xiaoqing
AU  - He X
AD  - Department of Orthopedics, Kunming General Hospital of Chengdu Military Region.
FAU - Song, Muguo
AU  - Song M
AD  - Department of Orthopedics, Kunming General Hospital of Chengdu Military Region.
FAU - Chen, Xueqiu
AU  - Chen X
AD  - Kunming Medical University, Kunming Yunnan, 650504, P. R. China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
OTO - NOTNLM
OT  - Molecular mechanism
OT  - Nuclear factor kappaB
OT  - Osteoclast differentiation
OT  - Osteomyelitis
OT  - Staphylococcal peptidoglycan
EDAT- 2016/10/08 00:00
MHDA- 2016/10/08 00:01
CRDT- 2018/05/23 06:00
PHST- 2018/05/23 06:00 [entrez]
PHST- 2016/10/08 00:00 [pubmed]
PHST- 2016/10/08 00:01 [medline]
AID - 10.7507/1002-1892.20160254 [doi]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2016 Oct 8;30(10):1244-1248. doi: 
      10.7507/1002-1892.20160254.