PMID- 29788425
OWN - NLM
STAT- MEDLINE
DCOM- 20200414
LR  - 20200414
IS  - 1460-2385 (Electronic)
IS  - 0931-0509 (Print)
IS  - 0931-0509 (Linking)
VI  - 34
IP  - 6
DP  - 2019 Jun 1
TI  - Patients with advanced chronic kidney disease and vascular calcification have a 
      large hydrodynamic radius of secondary calciprotein particles.
PG  - 992-1000
LID - 10.1093/ndt/gfy117 [doi]
AB  - BACKGROUND: The size of secondary calciprotein particles (CPP2) and the speed of 
      transformation (T50) from primary calciprotein particles (CPP1) to CPP2 in serum 
      may be associated with vascular calcification (VC) in patients with chronic 
      kidney disease (CKD). METHODS: We developed a high throughput, microplate-based 
      assay using dynamic light scattering (DLS) to measure the transformation of CPP1 
      to CPP2, hydrodynamic radius (Rh) of CPP1 and CPP2, T50 and aggregation of CPP2. 
      We used this DLS assay to test the hypothesis that a large Rh of CPP2 and/or a 
      fast T50 are associated with VC in 45 participants with CKD Stages 4-5 (22 
      without VC and 23 with VC) and 17 healthy volunteers (HV). VC was defined as a 
      Kauppila score >6 or an Adragao score >/=3. RESULTS: CKD participants with VC had 
      larger cumulants Rh of CPP2 370 nm [interquartile range (IQR) 272-566] compared 
      with CKD participants without VC [212 nm (IQR 169-315)] and compared with HV 
      [168 nm (IQR 145-352), P < 0.01 for each]. More CPP2 were in aggregates in CKD 
      participants with VC than those without VC (70% versus 36%). The odds of having 
      VC increased by 9% with every 10 nm increase in the Rh of CPP2, after adjusting 
      for age, diabetes, serum calcium and phosphate [odds ratio 1.09, 95% confidence 
      interval (CI) 1.03, 1.16, P = 0.005]. The area under the receiver operating 
      characteristic curve for VC of CPP2 size was 0.75 (95% CI 0.60, 0.90). T50 was 
      similar in CKD participants with and without VC, although both groups had a lower 
      T50 than HV. CONCLUSIONS: Rh of CPP2, but not T50, is independently associated 
      with VC in patients with CKD Stages 4-5.
CI  - (c) The Author(s) 2018. Published by Oxford University Press on behalf of ERA-EDTA. 
      All rights reserved.
FAU - Chen, Wei
AU  - Chen W
AD  - Department of Medicine, University of Rochester School of Medicine and Dentistry, 
      Rochester, NY, USA.
FAU - Anokhina, Viktoriya
AU  - Anokhina V
AD  - Department of Biochemistry and Biophysics, University of Rochester School of 
      Medicine and Dentistry, Rochester, NY, USA.
FAU - Dieudonne, Gregory
AU  - Dieudonne G
AD  - Department of Radiology, University of Rochester School of Medicine and 
      Dentistry, Rochester, NY, USA.
FAU - Abramowitz, Matthew K
AU  - Abramowitz MK
AD  - Department of Medicine, Albert Einstein College of Medicine, Bronx, NY, USA.
FAU - Kashyap, Randeep
AU  - Kashyap R
AD  - Department of Surgery, University of Rochester School of Medicine and Dentistry, 
      Rochester, NY, USA.
FAU - Yan, Chen
AU  - Yan C
AD  - Department of Medicine, University of Rochester School of Medicine and Dentistry, 
      Rochester, NY, USA.
FAU - Wu, Tong Tong
AU  - Wu TT
AD  - Department of Biostatistics and Computational Biology, University of Rochester 
      School of Medicine and Dentistry, Rochester, NY, USA.
FAU - de Mesy Bentley, Karen L
AU  - de Mesy Bentley KL
AD  - Department of Pathology and Laboratory Medicine, University of Rochester School 
      of Medicine and Dentistry, Rochester, NY, USA.
FAU - Miller, Benjamin L
AU  - Miller BL
AD  - Department of Biochemistry and Biophysics, University of Rochester School of 
      Medicine and Dentistry, Rochester, NY, USA.
AD  - Department of Dermatology, University of Rochester School of Medicine and 
      Dentistry, Rochester, NY, USA.
AD  - Department of Biomedical Engineering, University of Rochester School of Medicine 
      and Dentistry, Rochester, NY, USA.
FAU - Bushinsky, David A
AU  - Bushinsky DA
AD  - Department of Medicine, University of Rochester School of Medicine and Dentistry, 
      Rochester, NY, USA.
LA  - eng
GR  - KL2 TR001999/TR/NCATS NIH HHS/United States
GR  - R01 DK075462/DK/NIDDK NIH HHS/United States
GR  - S10 RR026501/RR/NCRR NIH HHS/United States
GR  - S10 RR027241/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nephrol Dial Transplant
JT  - Nephrology, dialysis, transplantation : official publication of the European 
      Dialysis and Transplant Association - European Renal Association
JID - 8706402
RN  - 0 (Phosphates)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adult
MH  - Calcium/*blood
MH  - Cross-Sectional Studies
MH  - Diabetes Mellitus
MH  - Female
MH  - Glomerular Filtration Rate
MH  - Humans
MH  - Hydrodynamics
MH  - Light
MH  - Male
MH  - Middle Aged
MH  - Odds Ratio
MH  - Phosphates/*blood
MH  - Photometry/*methods
MH  - Regression Analysis
MH  - Renal Insufficiency, Chronic/*blood/complications
MH  - Scattering, Radiation
MH  - Vascular Calcification/*blood/complications
MH  - Young Adult
PMC - PMC6545469
OTO - NOTNLM
OT  - calcification propensity
OT  - calciprotein particle
OT  - chronic kidney disease
OT  - mineral metabolism
OT  - vascular calcification
EDAT- 2018/05/23 06:00
MHDA- 2020/04/15 06:00
PMCR- 2019/05/16
CRDT- 2018/05/23 06:00
PHST- 2018/02/16 00:00 [received]
PHST- 2018/05/23 06:00 [pubmed]
PHST- 2020/04/15 06:00 [medline]
PHST- 2018/05/23 06:00 [entrez]
PHST- 2019/05/16 00:00 [pmc-release]
AID - 4996711 [pii]
AID - gfy117 [pii]
AID - 10.1093/ndt/gfy117 [doi]
PST - ppublish
SO  - Nephrol Dial Transplant. 2019 Jun 1;34(6):992-1000. doi: 10.1093/ndt/gfy117.