PMID- 29792818
OWN - NLM
STAT- MEDLINE
DCOM- 20190503
LR  - 20190503
IS  - 1936-1238 (Electronic)
IS  - 1936-122X (Linking)
VI  - 47
DP  - 2018 May 20
TI  - Substrate-Induced Formation of Ribosomal Decoding Center for Accurate and Rapid 
      Genetic Code Translation.
PG  - 525-548
LID - 10.1146/annurev-biophys-060414-034148 [doi]
AB  - Accurate translation of genetic information is crucial for synthesis of 
      functional proteins in all organisms. We use recent experimental data to discuss 
      how induced fit affects accuracy of initial codon selection on the ribosome by 
      aminoacyl transfer RNA in ternary complex ( T(3)) with elongation factor Tu 
      (EF-Tu) and guanosine-5'-triphosphate (GTP). We define actual accuracy ([Formula: 
      see text]) of a particular protein synthesis system as its current accuracy and 
      the effective selectivity ([Formula: see text]) as [Formula: see text] in the 
      limit of zero ribosomal binding affinity for T(3). Intrinsic selectivity 
      ([Formula: see text]), defined as the upper thermodynamic limit of [Formula: see 
      text], is determined by the free energy difference between near-cognate and 
      cognate T(3) in the pre-GTP hydrolysis state on the ribosome. [Formula: see text] 
      is much larger than [Formula: see text], suggesting the possibility of a 
      considerable increase in [Formula: see text] and [Formula: see text] at 
      negligible kinetic cost. Induced fit increases [Formula: see text] and [Formula: 
      see text] without affecting [Formula: see text], and aminoglycoside antibiotics 
      reduce [Formula: see text] and [Formula: see text] at unaltered [Formula: see 
      text].
FAU - Pavlov, Michael Y
AU  - Pavlov MY
AD  - Department of Cell and Molecular Biology, Uppsala University, Uppsala 75124, 
      Sweden; email: ehrenberg@xray.bmc.uu.se.
FAU - Ehrenberg, Mans
AU  - Ehrenberg M
AD  - Department of Cell and Molecular Biology, Uppsala University, Uppsala 75124, 
      Sweden; email: ehrenberg@xray.bmc.uu.se.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - Annu Rev Biophys
JT  - Annual review of biophysics
JID - 101469708
SB  - IM
MH  - Genetic Code/*genetics
MH  - Humans
MH  - Ribosomes/*chemistry
OTO - NOTNLM
OT  - induced fit
OT  - initial selection
OT  - ribosome
OT  - tautomers
OT  - translation accuracy
EDAT- 2018/05/25 06:00
MHDA- 2019/05/06 06:00
CRDT- 2018/05/25 06:00
PHST- 2018/05/25 06:00 [entrez]
PHST- 2018/05/25 06:00 [pubmed]
PHST- 2019/05/06 06:00 [medline]
AID - 10.1146/annurev-biophys-060414-034148 [doi]
PST - ppublish
SO  - Annu Rev Biophys. 2018 May 20;47:525-548. doi: 
      10.1146/annurev-biophys-060414-034148.