PMID- 29792866
OWN - NLM
STAT- MEDLINE
DCOM- 20181105
LR  - 20181105
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 502
IP  - 2
DP  - 2018 Jul 12
TI  - Cardiac extracellular matrix hydrogel together with or without inducer cocktail 
      improves human adipose tissue-derived stem cells differentiation into 
      cardiomyocyte-like cells.
PG  - 215-225
LID - S0006-291X(18)31216-6 [pii]
LID - 10.1016/j.bbrc.2018.05.147 [doi]
AB  - Studies have demonstrated that differentiation of stem cells into cardiomyocytes 
      is a complex phenomenon that requires sufficient inducing factors at various time 
      points. Cardiac extracellular matrix (cECM) could provide tissue specific 
      microenvironment and act as an inductive template for efficient cell 
      differentiation. The aim of this study was to investigate the effect of cECM on 
      differentiation of human adipose tissue-derived stem cells (hADSCs) into 
      cardiomyocytes using cECM hydrogel in combination with a cardiac inductive 
      cocktail. hADSCs were cultured on ECM-coated plates with and without inductive 
      cocktail for 3weeks. qRT-PCR and western blot analysis were used to evaluate the 
      expression pattern of special cardiac genes and proteins. When hADSCs were 
      cultured in the presence of cECM cardiac genes including GATA4, HAND1, HAND2, 
      NKX2.5, Troponin I, betaMHC, Connexin43 were highly expressed in differentiated 
      cells. Also Connexin43, cTnI and betaMHC proteins were expressed as well. We could 
      show that cECM by itself could affect viability, proliferation and 
      differentiation of hADSCs. However, combination of cECM with a cardiac inducing 
      cocktail could improve the results.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Baghalishahi, Masoumeh
AU  - Baghalishahi M
AD  - Department of Biology and Anatomical Sciences, School of Medicine, Shahid 
      Beheshti University of Medical Sciences, Tehran, Iran.
FAU - Efthekhar-Vaghefi, Seyed Hasan
AU  - Efthekhar-Vaghefi SH
AD  - Department of Anatomical Science, School of Medicine, Afzalipour University of 
      Medical Science, Kerman, Iran. Electronic address: sheftekharvaghefi@kmu.ac.ir.
FAU - Piryaei, Abbas
AU  - Piryaei A
AD  - Department of Biology and Anatomical Sciences, School of Medicine, Shahid 
      Beheshti University of Medical Sciences, Tehran, Iran; Department of Tissue 
      Engineering and Applied Cell Sciences, School of Advanced Technologies in 
      Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
FAU - Nematolahi-Mahani, S N
AU  - Nematolahi-Mahani SN
AD  - Department of Anatomical Science, School of Medicine, Afzalipour University of 
      Medical Science, Kerman, Iran.
FAU - Mollaei, Hamid Reza
AU  - Mollaei HR
AD  - Department of Medical Microbiology, School of Medicine, Afzalipour University of 
      Medical Science, Kerman, Iran.
FAU - Sadeghi, Yousef
AU  - Sadeghi Y
AD  - Department of Biology and Anatomical Sciences, School of Medicine, Shahid 
      Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: 
      yousefsadeghi@yahoo.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180528
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Hydrogels)
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - Adult Stem Cells/*cytology/*metabolism
MH  - Animals
MH  - Cell Differentiation/genetics/physiology
MH  - Cells, Cultured
MH  - Extracellular Matrix/genetics/metabolism
MH  - Gene Expression Profiling
MH  - Humans
MH  - Hydrogels
MH  - Myocardium/metabolism
MH  - Myocytes, Cardiac/*cytology/*metabolism
MH  - Rats
MH  - Regenerative Medicine
MH  - Stem Cell Niche/genetics/physiology
OTO - NOTNLM
OT  - Cardiac extracellular matrix
OT  - Cardiomyocyte-like cell
OT  - Differentiation
OT  - Human adipose tissue-derived stem cells
OT  - Tissue engineering
EDAT- 2018/05/25 06:00
MHDA- 2018/11/06 06:00
CRDT- 2018/05/25 06:00
PHST- 2018/05/17 00:00 [received]
PHST- 2018/05/20 00:00 [accepted]
PHST- 2018/05/25 06:00 [pubmed]
PHST- 2018/11/06 06:00 [medline]
PHST- 2018/05/25 06:00 [entrez]
AID - S0006-291X(18)31216-6 [pii]
AID - 10.1016/j.bbrc.2018.05.147 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2018 Jul 12;502(2):215-225. doi: 
      10.1016/j.bbrc.2018.05.147. Epub 2018 May 28.