PMID- 29795118
OWN - NLM
STAT- MEDLINE
DCOM- 20191125
LR  - 20191125
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 May 23
TI  - Profiling dendritic cell subsets in head and neck squamous cell tonsillar cancer 
      and benign tonsils.
PG  - 8030
LID - 10.1038/s41598-018-26193-y [doi]
LID - 8030
AB  - Dendritic cells (DCs) have a key role in orchestrating immune responses and are 
      considered important targets for immunotherapy against cancer. In order to 
      develop effective cancer vaccines, detailed knowledge of the micromilieu in 
      cancer lesions is warranted. In this study, flow cytometry and human 
      transcriptome arrays were used to characterize subsets of DCs in head and neck 
      squamous cell tonsillar cancer and compare them to their counterparts in benign 
      tonsils to evaluate subset-selective biomarkers associated with tonsillar cancer. 
      We describe, for the first time, four subsets of DCs in tonsillar cancer: 
      CD123(+) plasmacytoid DCs (pDC), CD1c(+), CD141(+), and CD1c(-)CD141(-) myeloid 
      DCs (mDC). An increased frequency of DCs and an elevated mDC/pDC ratio were shown 
      in malignant compared to benign tonsillar tissue. The microarray data 
      demonstrates characteristics specific for tonsil cancer DC subsets, including 
      expression of immunosuppressive molecules and lower expression levels of genes 
      involved in development of effector immune responses in DCs in malignant 
      tonsillar tissue, compared to their counterparts in benign tonsillar tissue. 
      Finally, we present target candidates selectively expressed by different DC 
      subsets in malignant tonsils and confirm expression of CD206/MRC1 and 
      CD207/Langerin on CD1c(+) DCs at protein level. This study descibes DC 
      characteristics in the context of head and neck cancer and add valuable steps 
      towards future DC-based therapies against tonsillar cancer.
FAU - Abolhalaj, Milad
AU  - Abolhalaj M
AD  - Department of Immunotechnology, Lund University, Lund, Sweden.
FAU - Askmyr, David
AU  - Askmyr D
AD  - Department of ORL, Head & Neck Surgery, Skane University Hospital, Lund, Sweden.
AD  - Department of Clinical Sciences, Lund University, Lund, Sweden.
FAU - Sakellariou, Christina Alexandra
AU  - Sakellariou CA
AD  - Department of Immunotechnology, Lund University, Lund, Sweden.
FAU - Lundberg, Kristina
AU  - Lundberg K
AD  - Department of Immunotechnology, Lund University, Lund, Sweden.
FAU - Greiff, Lennart
AU  - Greiff L
AD  - Department of ORL, Head & Neck Surgery, Skane University Hospital, Lund, Sweden.
AD  - Department of Clinical Sciences, Lund University, Lund, Sweden.
FAU - Lindstedt, Malin
AU  - Lindstedt M
AD  - Department of Immunotechnology, Lund University, Lund, Sweden. 
      malin.lindstedt@immun.lth.se.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180523
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Biomarkers, Tumor/*genetics
MH  - Carcinoma, Squamous Cell/*genetics/immunology/pathology
MH  - Cells, Cultured
MH  - Dendritic Cells/immunology/*metabolism/pathology
MH  - *Gene Expression Profiling
MH  - Humans
MH  - Palatine Tonsil/*metabolism/pathology
MH  - Tonsillar Neoplasms/*genetics/immunology/pathology
PMC - PMC5966442
COIS- The authors declare no competing interests.
EDAT- 2018/05/26 06:00
MHDA- 2019/11/26 06:00
PMCR- 2018/05/23
CRDT- 2018/05/26 06:00
PHST- 2017/11/24 00:00 [received]
PHST- 2018/04/19 00:00 [accepted]
PHST- 2018/05/26 06:00 [entrez]
PHST- 2018/05/26 06:00 [pubmed]
PHST- 2019/11/26 06:00 [medline]
PHST- 2018/05/23 00:00 [pmc-release]
AID - 10.1038/s41598-018-26193-y [pii]
AID - 26193 [pii]
AID - 10.1038/s41598-018-26193-y [doi]
PST - epublish
SO  - Sci Rep. 2018 May 23;8(1):8030. doi: 10.1038/s41598-018-26193-y.