PMID- 29796911
OWN - NLM
STAT- MEDLINE
DCOM- 20190415
LR  - 20190415
IS  - 1573-2568 (Electronic)
IS  - 0163-2116 (Print)
IS  - 0163-2116 (Linking)
VI  - 63
IP  - 11
DP  - 2018 Nov
TI  - Gut Microbiota Composition Before and After Use of Proton Pump Inhibitors.
PG  - 2940-2949
LID - 10.1007/s10620-018-5122-4 [doi]
AB  - BACKGROUND: Recently, problems associated with proton pump inhibitor (PPI) use 
      have begun to surface. PPIs influence the gut microbiota; therefore, PPI use may 
      increase the risk of enteric infections and cause bacterial translocation. In 
      this study, we investigated fecal microbiota composition, fecal organic acid 
      concentrations and pH, and gut bacteria in the blood of the same patients before 
      and after PPI use. METHODS: Twenty patients with reflux esophagitis based on 
      endoscopic examination received 8 weeks of treatment with PPIs. To analyze fecal 
      microbiota composition and gut bacteria in blood and organic acid concentrations, 
      16S and 23S rRNA-targeted quantitative RT-PCR and high-performance liquid 
      chromatography were conducted. RESULTS: Lactobacillus species were significantly 
      increased at both 4 and 8 weeks after PPI treatment compared with bacterial 
      counts before treatment (P = 0.011 and P = 0.002, respectively). Among 
      Lactobacillus spp., counts of the L. gasseri subgroup, L. fermentum, the L. 
      reuteri subgroup, and the L. ruminis subgroup were significantly increased at 4 
      and 8 weeks after treatment compared with counts before treatment. Streptococcus 
      species were also significantly increased at 4 and 8 weeks after PPI treatment 
      compared with counts before treatment (P < 0.01 and P < 0.001, respectively). 
      There was no significant difference in the total organic acid concentrations 
      before and after PPI treatment. Detection rates of bacteria in blood before and 
      after PPI treatment were 22 and 28%, respectively, with no significant 
      differences. CONCLUSIONS: Our quantitative RT-PCR results showed that gut 
      dysbiosis was caused by PPI use, corroborating previous results obtained by 
      metagenomic analysis.
FAU - Hojo, Mariko
AU  - Hojo M
AUID- ORCID: 0000-0001-9671-5585
AD  - Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1 
      Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan. mhojo@juntendo.ac.jp.
FAU - Asahara, Takashi
AU  - Asahara T
AD  - Probiotics Research Laboratory, Juntendo University Graduate School of Medicine, 
      Tokyo, Japan.
AD  - Yakult Central Institute, Tokyo, Japan.
FAU - Nagahara, Akihito
AU  - Nagahara A
AD  - Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1 
      Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
FAU - Takeda, Tsutomu
AU  - Takeda T
AD  - Department of Gastroenterology, Juntendo Tokyo Koto Geriatric Medical Center, 
      Tokyo, Japan.
FAU - Matsumoto, Kohei
AU  - Matsumoto K
AD  - Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1 
      Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
FAU - Ueyama, Hiroya
AU  - Ueyama H
AD  - Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1 
      Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
FAU - Matsumoto, Kenshi
AU  - Matsumoto K
AD  - Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1 
      Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
FAU - Asaoka, Daisuke
AU  - Asaoka D
AD  - Department of Gastroenterology, Juntendo Tokyo Koto Geriatric Medical Center, 
      Tokyo, Japan.
FAU - Takahashi, Takuya
AU  - Takahashi T
AD  - Probiotics Research Laboratory, Juntendo University Graduate School of Medicine, 
      Tokyo, Japan.
AD  - Yakult Central Institute, Tokyo, Japan.
FAU - Nomoto, Koji
AU  - Nomoto K
AD  - Yakult Central Institute, Tokyo, Japan.
AD  - Division of Molecular Microbiology, Tokyo University of Agriculture, Tokyo, 
      Japan.
FAU - Yamashiro, Yuichiro
AU  - Yamashiro Y
AD  - Probiotics Research Laboratory, Juntendo University Graduate School of Medicine, 
      Tokyo, Japan.
FAU - Watanabe, Sumio
AU  - Watanabe S
AD  - Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1 
      Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
LA  - eng
PT  - Journal Article
PT  - Observational Study
DEP - 20180524
PL  - United States
TA  - Dig Dis Sci
JT  - Digestive diseases and sciences
JID - 7902782
RN  - 0 (Carboxylic Acids)
RN  - 0 (Proton Pump Inhibitors)
SB  - IM
CIN - Dig Dis Sci. 2018 Nov;63(11):2817-2819. PMID: 30218426
MH  - Aged
MH  - Blood/*microbiology
MH  - Carboxylic Acids/analysis
MH  - Dysbiosis/*chemically induced
MH  - Feces/chemistry/*microbiology
MH  - Female
MH  - Gastrointestinal Microbiome/*drug effects
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Proton Pump Inhibitors/*adverse effects
PMC - PMC6182435
OTO - NOTNLM
OT  - Bacterial translocation
OT  - Lactobacillus
OT  - Microbiota
OT  - Proton pump inhibitor
OT  - Streptococcus
COIS- The authors declare that they have no conflict of interest.
EDAT- 2018/05/26 06:00
MHDA- 2019/04/16 06:00
PMCR- 2018/05/24
CRDT- 2018/05/26 06:00
PHST- 2018/02/17 00:00 [received]
PHST- 2018/05/10 00:00 [accepted]
PHST- 2018/05/26 06:00 [pubmed]
PHST- 2019/04/16 06:00 [medline]
PHST- 2018/05/26 06:00 [entrez]
PHST- 2018/05/24 00:00 [pmc-release]
AID - 10.1007/s10620-018-5122-4 [pii]
AID - 5122 [pii]
AID - 10.1007/s10620-018-5122-4 [doi]
PST - ppublish
SO  - Dig Dis Sci. 2018 Nov;63(11):2940-2949. doi: 10.1007/s10620-018-5122-4. Epub 2018 
      May 24.