PMID- 29797252
OWN - NLM
STAT- MEDLINE
DCOM- 20190221
LR  - 20190221
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1792
DP  - 2018
TI  - Fluorescence In Situ Hybridization (FISH) in Multiple Myeloma.
PG  - 55-69
LID - 10.1007/978-1-4939-7865-6_5 [doi]
AB  - The application of fluorescence in situ hybridization (FISH) technology in 
      diagnosis and molecular classification of cancer-risk has become an essential 
      tool in the proceeding of personalized therapy. In multiple myeloma, the precise 
      FISH detection of numerical and structural genetic aberrations can be carried out 
      on metaphase chromosome spreads, interphase nuclei, and formalin fixed 
      paraffin-embedded (FFPE) tissues. To dissect highly complex cancer genomes, a 
      broad variety of novel DNA probes, which outpace supplies from commercial 
      resources on the market, are also crucial to the advanced translational 
      researches. Here, we provide the protocols for the creation of custom-made DNA 
      probes and for conducting hybridizations on various targeting cells and tissues.
FAU - Tian, Erming
AU  - Tian E
AD  - Myeloma Institute, University of Arkansas for Medical Sciences, Little Rock, AR, 
      USA. tianerming@uams.edu.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA Probes)
SB  - IM
MH  - Biomarkers, Tumor
MH  - Bone Marrow/metabolism/pathology
MH  - DNA Probes
MH  - Humans
MH  - Immunohistochemistry
MH  - *In Situ Hybridization, Fluorescence/methods
MH  - Interphase/genetics
MH  - Metaphase/genetics
MH  - Multiple Myeloma/diagnosis/*genetics
OTO - NOTNLM
OT  - Cancer cytogenetics
OT  - DNA probes
OT  - FISH
EDAT- 2018/05/26 06:00
MHDA- 2019/02/23 06:00
CRDT- 2018/05/26 06:00
PHST- 2018/05/26 06:00 [entrez]
PHST- 2018/05/26 06:00 [pubmed]
PHST- 2019/02/23 06:00 [medline]
AID - 10.1007/978-1-4939-7865-6_5 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2018;1792:55-69. doi: 10.1007/978-1-4939-7865-6_5.