PMID- 29797419
OWN - NLM
STAT- MEDLINE
DCOM- 20191031
LR  - 20191031
IS  - 1099-1263 (Electronic)
IS  - 0260-437X (Linking)
VI  - 38
IP  - 10
DP  - 2018 Oct
TI  - New approach to predict photoallergic potentials of chemicals based on murine 
      local lymph node assay.
PG  - 1316-1322
LID - 10.1002/jat.3643 [doi]
AB  - Photoallergic dermatitis, caused by pharmaceuticals and other consumer products, 
      is a very important issue in human health. However, S10 guidelines of the 
      International Conference on Harmonization do not recommend the existing 
      prediction methods for photoallergy because of their low predictability in human 
      cases. We applied local lymph node assay (LLNA), a reliable, quantitative skin 
      sensitization prediction test, to develop a new photoallergy prediction method. 
      This method involves a three-step approach: (1) ultraviolet (UV) absorption 
      analysis; (2) determination of no observed adverse effect level for skin 
      phototoxicity based on LLNA; and (3) photoallergy evaluation based on LLNA. 
      Photoallergic potential of chemicals was evaluated by comparing lymph node cell 
      proliferation among groups treated with chemicals with minimal effect levels of 
      skin sensitization and skin phototoxicity under UV irradiation (UV+) or non-UV 
      irradiation (UV-). A case showing significant difference (P < .05) in lymph node 
      cell proliferation rates between UV- and UV+ groups was considered positive for 
      photoallergic reaction. After testing 13 chemicals, seven human photoallergens 
      tested positive and the other six, with no evidence of causing photoallergic 
      dermatitis or UV absorption, tested negative. Among these chemicals, both 
      doxycycline hydrochloride and minocycline hydrochloride were tetracycline 
      antibiotics with different photoallergic properties, and the new method clearly 
      distinguished between the photoallergic properties of these chemicals. These 
      findings suggested high predictability of our method; therefore, it is promising 
      and effective in predicting human photoallergens.
CI  - Copyright (c) 2018 John Wiley & Sons, Ltd.
FAU - Maeda, Yosuke
AU  - Maeda Y
AUID- ORCID: 0000-0002-4480-9851
AD  - Chemicals Assessment and Research Center, Chemicals Evaluation and Research 
      Institute, Saitama, Japan.
AD  - The United Graduate School of Veterinary Science, Yamaguchi University, 
      Yamaguchi, Japan.
FAU - Hirosaki, Haruka
AU  - Hirosaki H
AD  - Chemicals Assessment and Research Center, Chemicals Evaluation and Research 
      Institute, Saitama, Japan.
FAU - Yamanaka, Hidenori
AU  - Yamanaka H
AD  - Chemicals Assessment and Research Center, Chemicals Evaluation and Research 
      Institute, Saitama, Japan.
FAU - Takeyoshi, Masahiro
AU  - Takeyoshi M
AD  - Chemicals Assessment and Research Center, Chemicals Evaluation and Research 
      Institute, Saitama, Japan.
LA  - eng
PT  - Journal Article
DEP - 20180523
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 0 (Allergens)
SB  - IM
MH  - Allergens/*toxicity
MH  - Animals
MH  - Dermatitis, Photoallergic/*etiology/pathology
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - *Local Lymph Node Assay
MH  - Lymph Nodes/*drug effects/pathology/radiation effects
MH  - Mice, Inbred CBA
MH  - No-Observed-Adverse-Effect Level
MH  - Predictive Value of Tests
MH  - *Ultraviolet Rays
OTO - NOTNLM
OT  - local lymph node assay
OT  - photoallergy
OT  - photosafety
OT  - skin phototoxicity
OT  - skin sensitization
EDAT- 2018/05/26 06:00
MHDA- 2019/11/02 06:00
CRDT- 2018/05/26 06:00
PHST- 2017/12/18 00:00 [received]
PHST- 2018/03/29 00:00 [revised]
PHST- 2018/03/29 00:00 [accepted]
PHST- 2018/05/26 06:00 [pubmed]
PHST- 2019/11/02 06:00 [medline]
PHST- 2018/05/26 06:00 [entrez]
AID - 10.1002/jat.3643 [doi]
PST - ppublish
SO  - J Appl Toxicol. 2018 Oct;38(10):1316-1322. doi: 10.1002/jat.3643. Epub 2018 May 
      23.