PMID- 29798595 OWN - NLM STAT- MEDLINE DCOM- 20180627 LR - 20211211 IS - 1002-1892 (Print) IS - 1002-1892 (Linking) VI - 31 IP - 11 DP - 2017 Nov 15 TI - [Effects of human urine-derived stem cells combined with chondroitinase ABC on the expressions of nerve growth factor and brain-derived neurotrophic factor in the spinal cord injury]. PG - 1377-1383 LID - 10.7507/1002-1892.201706082 [doi] AB - OBJECTIVE: To explore the effects of human urine-derived stem cells (hUSCs) and hUSCs combined with chondroitinase ABC (chABC) on the expressions of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) in the spinal cord injury (SCI) of rats, and to investigate the underlying mechanism. METHODS: hUSCs were cultured from human urine, and their phenotypes were detected by flow cytometry. The SCI model of rats were made via Allen method. Sixty Sprague Dawley rats were divided into 5 groups ( n=12): the sham operation group (group A), SCI group (group B), SCI+hUSCs group (group C), SCI+chABC group (group D), and SCI+hUSCs+chABC group (group E). Basso, Beattie, Bresnahan (BBB) score was used to measure the lower extremity motor function of rats in each group at 10, 20, and 30 days after operation. Real-time fluorescent quantitative PCR was used to detect the relative mRNA expressions of NGF and BDNF at 30 days. Meanwhile, the protein expression of NGF and BDNF were confirmed by immunohistochemistry staining. The relative protein expressions of Bax and Bcl-2 were detected by Western blot. RESULTS: The hUSCs were identified to have multipotential differentiation potential. At 10, 20, and 30 days, BBB score was significantly lower in group B than in groups A, C, D, and E, in groups C, D, and E than in group A, in groups C and D than in group E ( P<0.05). Real-time fluorescent quantitative PCR and immunohistochemistry staining demonstrated that the expressions of NGF and BDNF were significantly lower in group B than in groups A, C, D, and E, in groups C, D, and E than in group A, in groups C and D than in group E ( P<0.05); but there was no significant difference between groups C and D ( P>0.05). Western blot results indicated that the protein expression of Bax was significantly higher in group B than in groups A, C, D, and E, in groups C, D, and E than in group A, in groups C and D than in group E ( P<0.05). Meanwhile, the protein expression of Bcl-2 was significantly lower in group B than in groups A, C, D, and E, in groups C, D, and E than in group A, in groups C and D than in group E ( P<0.05). CONCLUSION: hUSCs can protect SCI and this positive effect can be enhanced by chABC; this neuro-protective effect may depend on promoting the expressions of NGF and BDNF, and suppressing the neuronal apoptosis. FAU - Li, Zhi AU - Li Z AD - Department of Orthopedics, Qingpu Branch of Zhongshan Hospital, Fudan University, 201700, P.R.China. FAU - Wu, Haihui AU - Wu H AD - Department of Orthopedics, Qingpu Branch of Zhongshan Hospital, Fudan University, 201700, P.R.China.1012023313@qq.com. LA - chi PT - Journal Article PL - China TA - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi JT - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery JID - 9425194 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 9061-61-4 (Nerve Growth Factor) RN - EC 4.2.2.20 (Chondroitin ABC Lyase) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/metabolism MH - Chondroitin ABC Lyase/*therapeutic use MH - Disease Models, Animal MH - Humans MH - Nerve Growth Factor/metabolism MH - Rats MH - Rats, Sprague-Dawley MH - Spinal Cord MH - Spinal Cord Injuries/*therapy MH - *Stem Cell Transplantation MH - Stem Cells MH - Urine/*cytology PMC - PMC8632575 OTO - NOTNLM OT - Human urine-derived stem cells OT - chondroitinase ABC OT - nerve growth factor OT - rat OT - spinal cord injury EDAT- 2018/05/26 06:00 MHDA- 2018/06/28 06:00 PMCR- 2017/11/01 CRDT- 2018/05/26 06:00 PHST- 2018/05/26 06:00 [entrez] PHST- 2018/05/26 06:00 [pubmed] PHST- 2018/06/28 06:00 [medline] PHST- 2017/11/01 00:00 [pmc-release] AID - zgxfcjwkzz-31-11-1377 [pii] AID - 10.7507/1002-1892.201706082 [doi] PST - ppublish SO - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2017 Nov 15;31(11):1377-1383. doi: 10.7507/1002-1892.201706082.