PMID- 29801604 OWN - NLM STAT- MEDLINE DCOM- 20180925 LR - 20191210 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 1025 DP - 2018 Sep 26 TI - Reduced graphene oxide/nile blue/gold nanoparticles complex-modified glassy carbon electrode used as a sensitive and label-free aptasensor for ratiometric electrochemical sensing of dopamine. PG - 154-162 LID - S0003-2670(18)30415-X [pii] LID - 10.1016/j.aca.2018.03.036 [doi] AB - In this work, glassy carbon electrode (GCE) surface was modified by drop-coating graphene oxide (GO) and nile blue (NB) to form GO/NB/GCE. By using a one-step coreduction treatment under cyclic voltammetry (CV) scanning, gold nanoparticles (AuNPs) were electrodeposited onto GO/NB/GCE surface, simultaneously generating reduced GO (rGO). AuNPs from the prepared rGO/NB/AuNPs/GCE was combined with 5'-SH-terminated aptamer of dopamine (DA) via Au-S coupling to fabricate aptamer-rGO/NB/AuNPs/GCE system. DA specifically combined with its aptamer modified on rGO/NB/AuNPs/GCE surface. CV, electrochemical impedance spectroscopy, square wave voltammetry responses of this system as the working electrode were measured. With the addition of DA, the peak current intensities located at -0.45 V (I(NB)) and 0.15 V (I(DA)) showed gradually decreased and increased changes, respectively. There was a good linear (R(2) = 0.9922) relationship between lg(I(DA)/I(NB)) and the logarithm of DA concentration (lgC(DA)) in the C(DA) range from 10 nM to 0.2 mM, showing a low detection limit of 1 nM. This system as a novel, sensitive and label-free aptasensor was used for ratiometric electrochemical sensing of DA. Experimental results verified that this aptasensor possessed high stability, selectivity and sensitivity towards DA detection, over potential interferents. This aptasensor efficiently determined DA in real biological samples, together with high detection recoveries of 97.0-104.0%. CI - Copyright (c) 2018 Elsevier B.V. All rights reserved. FAU - Jin, Hui AU - Jin H AD - College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, Shandong, 266071, PR China. FAU - Zhao, Chunqin AU - Zhao C AD - College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, Shandong, 266071, PR China. FAU - Gui, Rijun AU - Gui R AD - College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, Shandong, 266071, PR China. Electronic address: guirijun@163.com. FAU - Gao, Xiaohui AU - Gao X AD - College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, Shandong, 266071, PR China. FAU - Wang, Zonghua AU - Wang Z AD - College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, Shandong, 266071, PR China. Electronic address: wangzonghua@qdu.edu.cn. LA - eng PT - Evaluation Study PT - Journal Article DEP - 20180329 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 RN - 0 (Aptamers, Nucleotide) RN - 0 (Oxazines) RN - 0 (Oxides) RN - 2381-85-3 (Nile Blue) RN - 7440-44-0 (Carbon) RN - 7440-57-5 (Gold) RN - 7782-42-5 (Graphite) RN - VTD58H1Z2X (Dopamine) SB - IM MH - Aptamers, Nucleotide/*chemistry MH - Biosensing Techniques/instrumentation/*methods MH - Carbon/chemistry MH - Dopamine/*blood MH - Electrochemical Techniques/instrumentation/methods MH - Electrodes MH - Equipment Design MH - Gold/*chemistry MH - Graphite/*chemistry MH - Humans MH - Limit of Detection MH - Metal Nanoparticles/*chemistry MH - Oxazines/*chemistry MH - Oxides/chemistry MH - Young Adult OTO - NOTNLM OT - Aptasensor OT - Dopamine OT - Electrochemical sensor OT - Nile blue OT - Ratiometric EDAT- 2018/05/29 06:00 MHDA- 2018/09/27 06:00 CRDT- 2018/05/27 06:00 PHST- 2017/12/08 00:00 [received] PHST- 2018/02/25 00:00 [revised] PHST- 2018/03/12 00:00 [accepted] PHST- 2018/05/27 06:00 [entrez] PHST- 2018/05/29 06:00 [pubmed] PHST- 2018/09/27 06:00 [medline] AID - S0003-2670(18)30415-X [pii] AID - 10.1016/j.aca.2018.03.036 [doi] PST - ppublish SO - Anal Chim Acta. 2018 Sep 26;1025:154-162. doi: 10.1016/j.aca.2018.03.036. Epub 2018 Mar 29.