PMID- 29802255
OWN - NLM
STAT- MEDLINE
DCOM- 20191008
LR  - 20240314
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 May 25
TI  - CD11c+ M1-like macrophages (MPhis) but not CD206+ M2-like MPhi are involved in 
      folliculogenesis in mice ovary.
PG  - 8171
LID - 10.1038/s41598-018-25837-3 [doi]
LID - 8171
AB  - Macrophages (MPhis) are involved in folliculogenesis and ovulation. However, it is 
      unknown which type of MPhi, M1 or M2, plays a more essential role in the ovary. 
      CD206 or CD11c diphtheria toxin receptor transgenic (DTR) mice, which enable 
      depletion of CD206+ M2 MPhis and CD11c+ MPhi or CD11c+ Dendritic cells (DCs), 
      respectively, were used. Oocytes were used for in vitro fertilization and embryo 
      transfer. In vitro fertilized embryos derived from M2 MPhi depleted oocytes were 
      transferred to pseudo pregnant wild type mice. CD11c DTR mice were also used to 
      investigate the role of CD11c cells, M1 MPhi and DCs in folliculogenesis. In WT 
      mice, the proportion of CD206+ M2-like MPhis was not increased in follicular 
      induction, while that of CD11c+ M1-like MPhis was increased. In CD206 DTR mice, 
      folliculogenesis was normal and the ovulation number, fertilization rate, and 
      implantation rate were similar to those in WT mice. In CD11c DTR mice, 
      folliculogenesis was impaired with ovarian hemorrhage and the staining of 
      platelet derived growth factor-receptor beta (PDGF-Rbeta), a marker of pericytes, and 
      CD34, a marker of endothelial cells, was reduced. CD11c+ cells, M1 MPhis or DCs, 
      may be involved in folliculogenesis, while M2 MPhis are not involved in 
      folliculogenesis.
FAU - Ono, Yosuke
AU  - Ono Y
AD  - Department of Obstetrics and Gynecology, University of Toyama, Toyama, Japan.
FAU - Nagai, Miwako
AU  - Nagai M
AD  - Department of Obstetrics and Gynecology, University of Tokyo, Tokyo, Japan.
FAU - Yoshino, Osamu
AU  - Yoshino O
AD  - Department of Obstetrics and Gynecology, University of Toyama, Toyama, Japan.
FAU - Koga, Kaori
AU  - Koga K
AD  - Department of Obstetrics and Gynecology, University of Tokyo, Tokyo, Japan. 
      kawotan-tky@umin.ac.jp.
FAU - Nawaz, Allah
AU  - Nawaz A
AD  - First Department of Internal Medicine, University of Toyama, Toyama, Japan.
FAU - Hatta, Hideki
AU  - Hatta H
AD  - Department of Diagnostic Pathology, Graduate School of Medicine and 
      Pharmaceutical Sciences, University of Toyama, Toyama, Japan.
FAU - Nishizono, Hirofumi
AU  - Nishizono H
AD  - Division of Animal Resources and Development, University of Toyama, Toyama, 
      Japan.
FAU - Izumi, Gentaro
AU  - Izumi G
AUID- ORCID: 0000-0002-4546-1084
AD  - Department of Obstetrics and Gynecology, University of Tokyo, Tokyo, Japan.
FAU - Nakashima, Akitoshi
AU  - Nakashima A
AD  - Department of Obstetrics and Gynecology, University of Toyama, Toyama, Japan.
FAU - Imura, Johji
AU  - Imura J
AD  - Department of Diagnostic Pathology, Graduate School of Medicine and 
      Pharmaceutical Sciences, University of Toyama, Toyama, Japan.
FAU - Tobe, Kazuyuki
AU  - Tobe K
AD  - First Department of Internal Medicine, University of Toyama, Toyama, Japan.
FAU - Fujii, Tomoyuki
AU  - Fujii T
AD  - Department of Obstetrics and Gynecology, University of Tokyo, Tokyo, Japan.
FAU - Osuga, Yutaka
AU  - Osuga Y
AD  - Department of Obstetrics and Gynecology, University of Tokyo, Tokyo, Japan. 
      yutakaos-tky@umin.ac.jp.
FAU - Saito, Shigeru
AU  - Saito S
AD  - Department of Obstetrics and Gynecology, University of Toyama, Toyama, Japan. 
      s30saito@med.u-toyama.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180525
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (CD11c Antigen)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Mannose Receptor)
RN  - 0 (Mannose-Binding Lectins)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Animals
MH  - CD11c Antigen/*metabolism
MH  - Cell Count
MH  - Female
MH  - Fertilization
MH  - Lectins, C-Type/metabolism
MH  - Luteinization
MH  - Macrophages/*cytology/*metabolism
MH  - Mannose Receptor
MH  - Mannose-Binding Lectins/metabolism
MH  - Mice
MH  - Oocytes/metabolism
MH  - Ovarian Follicle/*physiology
MH  - Receptors, Cell Surface/metabolism
PMC - PMC5970206
COIS- The authors declare no competing interests.
EDAT- 2018/05/29 06:00
MHDA- 2019/10/09 06:00
PMCR- 2018/05/25
CRDT- 2018/05/27 06:00
PHST- 2017/11/14 00:00 [received]
PHST- 2018/04/20 00:00 [accepted]
PHST- 2018/05/27 06:00 [entrez]
PHST- 2018/05/29 06:00 [pubmed]
PHST- 2019/10/09 06:00 [medline]
PHST- 2018/05/25 00:00 [pmc-release]
AID - 10.1038/s41598-018-25837-3 [pii]
AID - 25837 [pii]
AID - 10.1038/s41598-018-25837-3 [doi]
PST - epublish
SO  - Sci Rep. 2018 May 25;8(1):8171. doi: 10.1038/s41598-018-25837-3.