PMID- 29802680
OWN - NLM
STAT- MEDLINE
DCOM- 20190610
LR  - 20240318
IS  - 2051-817X (Electronic)
IS  - 2051-817X (Linking)
VI  - 6
IP  - 10
DP  - 2018 May
TI  - Brain-derived neurotrophic factor acts at neurons of the subfornical organ to 
      influence cardiovascular function.
PG  - e13704
LID - 10.14814/phy2.13704 [doi]
LID - e13704
AB  - Brain-derived neurotrophic factor (BDNF), a neurotrophin traditionally associated 
      with neural plasticity, has more recently been implicated in fluid balance and 
      cardiovascular regulation. It is abundantly expressed in both the central nervous 
      system (CNS) and peripheral tissue, and is also found in circulation. Studies 
      suggest that circulating BDNF may influence the CNS through actions at the 
      subfornical organ (SFO), a circumventricular organ (CVO) characterized by the 
      lack of a normal blood-brain barrier (BBB). The SFO, well-known for its 
      involvement in cardiovascular regulation, has been shown to express BDNF mRNA and 
      mRNA for the TrkB receptor at which BDNF preferentially binds. This study was 
      undertaken to determine if: (1) BDNF influences the excitability of SFO neurons 
      in vitro; and (2) the cardiovascular consequences of direct administration of 
      BDNF into the SFO of anesthetized rats. Electrophysiological studies revealed 
      that bath application of BDNF (1 nmol/L) influenced the excitability of the 
      majority of neurons (60%, n = 13/22), the majority of which exhibited a membrane 
      depolarization (13.8 +/- 2.5 mV, n = 9) with the remaining affected cells 
      exhibiting hyperpolarizations (-11.1 +/- 2.3 mV, n = 4). BDNF microinjections into 
      the SFO of anesthetized rats caused a significant decrease in blood pressure 
      (mean [area under the curve] AUC = -364.4 +/- 89.0 mmHg x sec, n = 5) with no 
      effects on heart rate (mean AUC = -12.2 +/- 3.4, n = 5). Together these 
      observations suggest the SFO to be a CNS site at which circulating BDNF could 
      exert its effects on cardiovascular regulation.
CI  - (c) 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on 
      behalf of The Physiological Society and the American Physiological Society.
FAU - Black, Emily A E
AU  - Black EAE
AD  - Department of Biomedical and Molecular Sciences, Queen's University, Kingston, 
      Ontario, Canada.
FAU - Smith, Pauline M
AU  - Smith PM
AD  - Centre for Neuroscience Studies, Queen's University, Kingston, Ontario, Canada.
FAU - McIsaac, William
AU  - McIsaac W
AD  - Centre for Neuroscience Studies, Queen's University, Kingston, Ontario, Canada.
FAU - Ferguson, Alastair V
AU  - Ferguson AV
AD  - Centre for Neuroscience Studies, Queen's University, Kingston, Ontario, Canada.
LA  - eng
GR  - Canadian Institutes of Health Research/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Physiol Rep
JT  - Physiological reports
JID - 101607800
RN  - 0 (Bdnf protein, rat)
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Action Potentials/drug effects
MH  - Animals
MH  - Blood Pressure/*drug effects
MH  - Brain-Derived Neurotrophic Factor/administration & dosage/*physiology
MH  - Heart Rate/*drug effects
MH  - Male
MH  - Neurons/drug effects/*physiology
MH  - Rats, Sprague-Dawley
MH  - Subfornical Organ/drug effects/*physiology
PMC - PMC5974716
OTO - NOTNLM
OT  - blood pressure
OT  - patch clamp
OT  - subfornical organ
EDAT- 2018/05/29 06:00
MHDA- 2019/06/14 06:00
PMCR- 2018/05/20
CRDT- 2018/05/27 06:00
PHST- 2018/04/13 00:00 [received]
PHST- 2018/04/17 00:00 [accepted]
PHST- 2018/05/27 06:00 [entrez]
PHST- 2018/05/29 06:00 [pubmed]
PHST- 2019/06/14 06:00 [medline]
PHST- 2018/05/20 00:00 [pmc-release]
AID - PHY213704 [pii]
AID - 10.14814/phy2.13704 [doi]
PST - ppublish
SO  - Physiol Rep. 2018 May;6(10):e13704. doi: 10.14814/phy2.13704.