PMID- 29802792
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240330
IS  - 1613-4133 (Electronic)
IS  - 1613-4125 (Print)
IS  - 1613-4125 (Linking)
VI  - 62
IP  - 14
DP  - 2018 Jul
TI  - 6-Dihydroparadol, a Ginger Constituent, Enhances Cholesterol Efflux from 
      THP-1-Derived Macrophages.
PG  - e1800011
LID - 10.1002/mnfr.201800011 [doi]
LID - 1800011
AB  - SCOPE: Ginger is reported to be used for the prevention and treatment of 
      cardiovascular diseases (CVD). Cholesterol efflux from macrophage foam cells is 
      an important process in reverse cholesterol transport, whose increase may help to 
      prevent or treat CVD. In this study, we investigated the effects of 
      6-dihydroparadol from ginger on macrophage cholesterol efflux. METHODS AND 
      RESULTS: We show that 6-dihydroparadol concentration-dependently enhances both 
      apolipoprotein A1- and human plasma-mediated cholesterol efflux from 
      cholesterol-loaded THP-1-derived macrophages using macrophage cholesterol efflux 
      assay. 6-Dihydroparadol increases protein levels of both ATP-binding cassette 
      transporters A1 and G1 (ATP-binding cassette transporter A1 [ABCA1] and 
      ATP-binding cassette transporter G1 [ABCG1]) according to Western blot analysis. 
      The ABCA1 inhibitor probucol completely abolishes 6-dihydroparadol-enhanced 
      cholesterol efflux. Furthermore, increased ABCA1 protein levels in the presence 
      of 6-dihydroparadol were associated with both increased ABCA1 mRNA levels and 
      increased ABCA1 protein stability. Enhanced ABCG1 protein levels were only 
      associated with increased protein stability. Increased ABCA1 protein stability 
      appeared to be the result of a reduced proteasomal degradation of the transporter 
      in the presence of 6-dihydroparadol. CONCLUSION: We identified 6-dihydroparadol 
      from ginger as a novel promoter of cholesterol efflux from macrophages that 
      increases both ABCA1 and ABCG1 protein abundance. This newly identified 
      bioactivity might contribute to the antiatherogenic effects of ginger.
CI  - (c) 2018 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Wang, Dongdong
AU  - Wang D
AD  - Department of Pharmacognosy, University of Vienna, Althanstrasse 14, 1090, 
      Vienna, Austria.
AD  - Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, ul. 
      Postepu 36A, 05-552, Jastrzebiec, Poland.
AD  - Institute of Clinical Chemistry, University Hospital Zurich, University of 
      Zurich, Wagistrasse 14, 8952, Schlieren, Switzerland.
FAU - Hiebl, Verena
AU  - Hiebl V
AD  - Department of Pharmacognosy, University of Vienna, Althanstrasse 14, 1090, 
      Vienna, Austria.
FAU - Ladurner, Angela
AU  - Ladurner A
AD  - Department of Pharmacognosy, University of Vienna, Althanstrasse 14, 1090, 
      Vienna, Austria.
FAU - Latkolik, Simone L
AU  - Latkolik SL
AD  - Department of Pharmacognosy, University of Vienna, Althanstrasse 14, 1090, 
      Vienna, Austria.
FAU - Bucar, Franz
AU  - Bucar F
AD  - Department of Pharmacognosy, Institute of Pharmaceutical Sciences, University of 
      Graz, 8010, Graz, Austria.
FAU - Heiss, Elke H
AU  - Heiss EH
AD  - Department of Pharmacognosy, University of Vienna, Althanstrasse 14, 1090, 
      Vienna, Austria.
FAU - Dirsch, Verena M
AU  - Dirsch VM
AD  - Department of Pharmacognosy, University of Vienna, Althanstrasse 14, 1090, 
      Vienna, Austria.
FAU - Atanasov, Atanas G
AU  - Atanasov AG
AUID- ORCID: 0000-0003-2545-0967
AD  - Department of Pharmacognosy, University of Vienna, Althanstrasse 14, 1090, 
      Vienna, Austria.
AD  - Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, ul. 
      Postepu 36A, 05-552, Jastrzebiec, Poland.
LA  - eng
GR  - P 25971/FWF_/Austrian Science Fund FWF/Austria
GR  - H-297332/2014/Vienna Anniversary Foundation for Higher Education 
      (Hochschuljubilaumsstiftung der Stadt Wien)/
GR  - GZ402.000/00014-WF/V/6/2016/Austrian Federal Ministry of Science, Research and 
      Economy/
GR  - European Regional Development Fund/
GR  - 05-1/KNOW2/2015/Ministry of Science and Higher Education/
GR  - P25971-B23/Austrian Science Fund/
PT  - Journal Article
DEP - 20180625
PL  - Germany
TA  - Mol Nutr Food Res
JT  - Molecular nutrition & food research
JID - 101231818
SB  - IM
PMC - PMC6099374
MID - EMS78584
OTO - NOTNLM
OT  - ATP-binding cassette transporter A1
OT  - ATP-binding cassette transporter G1
OT  - atherosclerosis
OT  - cardiovascular diseases
OT  - cholesterol efflux
EDAT- 2018/05/29 06:00
MHDA- 2018/05/29 06:01
PMCR- 2018/08/20
CRDT- 2018/05/27 06:00
PHST- 2018/04/24 00:00 [revised]
PHST- 2018/01/05 00:00 [received]
PHST- 2018/05/29 06:00 [pubmed]
PHST- 2018/05/29 06:01 [medline]
PHST- 2018/05/27 06:00 [entrez]
PHST- 2018/08/20 00:00 [pmc-release]
AID - MNFR3246 [pii]
AID - 10.1002/mnfr.201800011 [doi]
PST - ppublish
SO  - Mol Nutr Food Res. 2018 Jul;62(14):e1800011. doi: 10.1002/mnfr.201800011. Epub 
      2018 Jun 25.