PMID- 29803846
OWN - NLM
STAT- MEDLINE
DCOM- 20190114
LR  - 20190114
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 121
DP  - 2018 Aug
TI  - Paracoccidioidomycosis: Detection of Paracoccidioides brasiliensis  genome in 
      biological samples by quantitative chain reaction polymerase (qPCR).
PG  - 359-362
LID - S0882-4010(17)31268-8 [pii]
LID - 10.1016/j.micpath.2018.05.039 [doi]
AB  - Paracoccidioidomycosis (PCM) is a chronic mycosis caused by the saprobic and 
      dimorphic species Paracoccidioides brasiliensis and P. lutzii. This disease is 
      prevalent in Latin American countries. PCM appears as a relevant concern and 
      challenge for the mycologists, since until now there is no a methodology suitable 
      for an efficient and safe diagnosis and species identification. Thus, the present 
      study aimed to validate a methodology for PCM s diagnosis, using quantitative 
      Polymerase Chain Reaction (qPCR) through target amplification of the gene 
      encoding the recombinant protein Pb27, a common protein to the both species 
      Paracoccidioides brasiliensis and P. lutzii. The experiments were performed 
      in vitro to determine the specificity, efficiency and detection limit of qPCR 
      assay, using specific primers and probe, which sequences were subject to a patent 
      deposited in Brazilian CTIT, under the registration number: BR1020160078830. 
      According to the results the technique showed sensitivity of 94% and specificity 
      of 100%, demonstrating that it will be possible to develop a new fast and safe 
      diagnostic PCM and can be standardized in order to present a low cost, accessible 
      to the patient served by the public health system in Brazil and Latin America.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Rocha-Silva, Fabiana
AU  - Rocha-Silva F
AD  - Nucleo de Pos-Graduacao e Pesquisa da Santa Casa de Belo Horizonte, MG, Brazil 
      Rua Domingos Vieira, 590 - 30150240, Belo Horizonte, Brazil.
FAU - Maria de Figueiredo, Sonia
AU  - Maria de Figueiredo S
AD  - Nucleo de Pos-Graduacao e Pesquisa da Santa Casa de Belo Horizonte, MG, Brazil 
      Rua Domingos Vieira, 590 - 30150240, Belo Horizonte, Brazil; Departamento de 
      Alimentos, Escola de Nutricao, Universidade Federal de Ouro Preto, Ouro Preto, 
      MG, Brazil.
FAU - Rutren La Santrer, Emanoelle Fernandes
AU  - Rutren La Santrer EF
AD  - Nucleo de Pos-Graduacao e Pesquisa da Santa Casa de Belo Horizonte, MG, Brazil 
      Rua Domingos Vieira, 590 - 30150240, Belo Horizonte, Brazil.
FAU - Machado, Amanda Sanchez
AU  - Machado AS
AD  - Nucleo de Pos-Graduacao e Pesquisa da Santa Casa de Belo Horizonte, MG, Brazil 
      Rua Domingos Vieira, 590 - 30150240, Belo Horizonte, Brazil.
FAU - Fernandes, Blenda
AU  - Fernandes B
AD  - Nucleo de Pos-Graduacao e Pesquisa da Santa Casa de Belo Horizonte, MG, Brazil 
      Rua Domingos Vieira, 590 - 30150240, Belo Horizonte, Brazil.
FAU - Assuncao, Claudia Barbosa
AU  - Assuncao CB
AD  - Nucleo de Pos-Graduacao e Pesquisa da Santa Casa de Belo Horizonte, MG, Brazil 
      Rua Domingos Vieira, 590 - 30150240, Belo Horizonte, Brazil.
FAU - Goes, Alfredo Miranda
AU  - Goes AM
AD  - Departamento de Patologia Geral, Instituto de Ciencias Biologicas, Universidade 
      Federal de Minas Gerais, Brazil.
FAU - Caligiorne, Rachel Basques
AU  - Caligiorne RB
AD  - Nucleo de Pos-Graduacao e Pesquisa da Santa Casa de Belo Horizonte, MG, Brazil 
      Rua Domingos Vieira, 590 - 30150240, Belo Horizonte, Brazil. Electronic address: 
      rachelbc@santacasabh.org.br.
LA  - eng
PT  - Journal Article
DEP - 20180524
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (DNA, Fungal)
SB  - IM
MH  - Brazil
MH  - Bronchoalveolar Lavage Fluid/microbiology
MH  - DNA, Fungal/*isolation & purification
MH  - Female
MH  - Humans
MH  - Latin America/epidemiology
MH  - Male
MH  - Paracoccidioides/*genetics/isolation & purification
MH  - Paracoccidioidomycosis/*diagnosis/*epidemiology
MH  - Prevalence
MH  - Real-Time Polymerase Chain Reaction
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - Diagnosis
OT  - P. lutzii
OT  - Paracoccidioidomycosis
OT  - Paracocciodioides brasiliensis
OT  - Protein Pb27
OT  - Quantitative PCR
OT  - qPCR
EDAT- 2018/05/29 06:00
MHDA- 2019/01/15 06:00
CRDT- 2018/05/28 06:00
PHST- 2017/10/04 00:00 [received]
PHST- 2018/05/17 00:00 [revised]
PHST- 2018/05/23 00:00 [accepted]
PHST- 2018/05/29 06:00 [pubmed]
PHST- 2019/01/15 06:00 [medline]
PHST- 2018/05/28 06:00 [entrez]
AID - S0882-4010(17)31268-8 [pii]
AID - 10.1016/j.micpath.2018.05.039 [doi]
PST - ppublish
SO  - Microb Pathog. 2018 Aug;121:359-362. doi: 10.1016/j.micpath.2018.05.039. Epub 
      2018 May 24.